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Image Search Results
Journal: Frontiers in Molecular Neuroscience
Article Title: Trait sensitivity to negative feedback in rats is associated with increased expression of serotonin 5-HT 2A receptors in the ventral hippocampus
doi: 10.3389/fnmol.2023.1092864
Figure Lengend Snippet: Analysis of the differences in the expression of serotonin 5-HT receptors in the ventral hippocampus (vHipp) and dorsal hippocampus (dHipp) of rats trait insensitive and trait sensitive to negative feedback (NF). Panels (A–D) demonstrate the mRNA levels of genes encoding the Htr1a (A) , Htr2a (B) , Htr2c (C) , and Htr7 (D) serotonin receptors in the vHipp of trait NF-sensitive and trait NF-insensitive rats. Panels (E–H) demonstrate the mRNA levels of genes encoding the Htr1a (E) , Htr2a (F) , Htr2c (G) , and Htr7 (H) serotonin receptors in the dHipp of trait NF-sensitive and trait NF-insensitive rats. Panels I and J demonstrate the protein levels of 5-HT 2A receptors in the vHipp (I) and the protein levels of 5-HT 7 receptors in the dHipp (J) of trait NF-sensitive and trait NF-insensitive rats. Panels K and L demonstrate the levels of miR-16-5p (K) and miR-15b-5p (L) in the vHipp of trait NF-sensitive and trait NF-insensitive rats. Data are presented as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001 vs. the NF-sensitive group.
Article Snippet: RT–qPCR was performed by using Quant Studio 3 (
Techniques: Expressing
Journal: Frontiers in Psychiatry
Article Title: Histone deacetylase inhibitors mitigate antipsychotic risperidone-induced motor side effects in aged mice and in a mouse model of Alzheimer’s disease
doi: 10.3389/fpsyt.2022.1020831
Figure Lengend Snippet: Effects of histone deacetylase (HDAC) inhibitors on histone acetylation at the 5htr2a and Drd2 gene promoters in the striatum in young and aged mice. (A) No significant differences of H3K27ac binding at the 5htr2a promoter between young and aged group was found, but a significant increase in H3K27ac binding at the 5ht2a promoter in the striatum was found in aged and young mice co-treated with RIS and VPA or MS-275 compared to those treated with RIS alone. (B) H3K27ac binding at the Drd2 promoter was significantly decreased in aged mice as compared to young mice in Veh treated group. However, H3K27ac binding at the Drd2 promoter in the striatum was observed to be significantly increased in aged mice co-treated with RIS and HDAC inhibitors VPA or MS-275 when compared to those treated with Veh alone. Data are presented as mean ± SEM, ( n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Article Snippet: Blots were blocked for 1-hour with 5% non-fat dry milk (Biorad, 1706404) and incubated using 1:1,000
Techniques: Histone Deacetylase Assay, Binding Assay