Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Alpha‐actinin‐4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy

doi: 10.1186/s12958-021-00733-0

Figure Lengend Snippet: ACTN4 expression in placental CTBs. a IHC staining of ACTN4 in the human first trimester placenta villi. CTBs were stained for CK7; Scale bars: 100 μm. b EdU labeling in explant cultures. Villous explants isolated from the first trimester placentas were treated with the control siRNA (si-NC) or ACTN4 siRNA (si-ACTN4). Representative pictures showed EdU (pink) labeling in the villous cytotrophoblasts of floating villi following 72-h treatment. Nuclei were counterstained with DAPI (blue). Scale bars: 100 μm. All data are presented as the means ± SEM of three independent experiments. * p < 0.05

Article Snippet: The immortalized human trophoblast cell lines, HTR8/SVneo (American Type Culture Collection, Manassas, VA, USA), were cultured in RPMI-1640 (Gibco, Carlsbad, CA, USA) medium supplemented with 10 % fetal bovine serum (FBS; Gemini, California, USA) under standard conditions (37 °C, 5 % CO 2, humidified atmosphere). si-ACTN4 (50 nM, 5′-GUUCAUCGUCCAUACCAUC-3′ [ ]) and a negative control siRNA were synthesized by GenePharma, Inc. (Shanghai, China).

Techniques: Expressing, Immunohistochemistry, Staining, Labeling, Isolation, Control

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Alpha‐actinin‐4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy

doi: 10.1186/s12958-021-00733-0

Figure Lengend Snippet: ACTN4 expression in the process of CTB differentiation into STBs. a Immunofluorescence staining for ACTN4 (red) and CK7 (green) in the freshly isolated primary human CTB cells. Staining was performed after 3 or 48 h of culture. Nuclei were counterstained with DAPI (blue). White dashed circles and white arrows indicate syncytialized cells and unsyncytialized cells, respectively. Scale bars: 100 μm. b Western blotting for ACTN4 and GCM-1 (a syncytiotrophoblast marker) in the primary human CTB cells after 3 or 48 h of culture. All data are presented as the means ± SEM of three independent experiments. ** P < 0.01; *** P < 0.001

Article Snippet: The immortalized human trophoblast cell lines, HTR8/SVneo (American Type Culture Collection, Manassas, VA, USA), were cultured in RPMI-1640 (Gibco, Carlsbad, CA, USA) medium supplemented with 10 % fetal bovine serum (FBS; Gemini, California, USA) under standard conditions (37 °C, 5 % CO 2, humidified atmosphere). si-ACTN4 (50 nM, 5′-GUUCAUCGUCCAUACCAUC-3′ [ ]) and a negative control siRNA were synthesized by GenePharma, Inc. (Shanghai, China).

Techniques: Expressing, Immunofluorescence, Staining, Isolation, Western Blot, Marker

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Alpha‐actinin‐4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy

doi: 10.1186/s12958-021-00733-0

Figure Lengend Snippet: ACTN4 regulation of the AKT/GSK3β/Snail pathway in trophoblasts. Western blotting for ACTN4, p-AKT Ser473 , AKT, p-GSK3β, Snail, N-cadherin, and Vimentin in the first trimester villi (left panel) and HTR-8/SVneo cells (right panel) transfected with si-NC or si-ACTN4 for 72 h. All data are presented as the means ± SEM of three independent experiments. * P < 0.05; ** P < 0.01

Article Snippet: The immortalized human trophoblast cell lines, HTR8/SVneo (American Type Culture Collection, Manassas, VA, USA), were cultured in RPMI-1640 (Gibco, Carlsbad, CA, USA) medium supplemented with 10 % fetal bovine serum (FBS; Gemini, California, USA) under standard conditions (37 °C, 5 % CO 2, humidified atmosphere). si-ACTN4 (50 nM, 5′-GUUCAUCGUCCAUACCAUC-3′ [ ]) and a negative control siRNA were synthesized by GenePharma, Inc. (Shanghai, China).

Techniques: Western Blot, Transfection

Journal: Pharmacological Reports

Article Title: Maternal feeding patterns affect the offspring’s brain: focus on serotonin 5-HT 2C and 5-HT 2A receptors

doi: 10.1007/s43440-021-00298-0

Figure Lengend Snippet: The levels of the 5-HT 2C receptor in the prefrontal cortex, nucleus accumbens, dorsal striatum and hippocampus of male and female offspring whose mothers were fed a high-fat diet (HFD), high-carbohydrate diet (HCD), mixed diet (MD) or standard diet (SD) at postnatal day (PND) 28. n = 8 rats/group for the prefrontal cortex and nucleus accumbens; n = 7 rats/group for the dorsal striatum and hippocampus). Significance was determined using two-way ANOVA followed Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001 versus the corresponding SD group

Article Snippet: The concentrations of 5-HT 2C and 5-HT 2A receptors in the selected brain structures were measured using ELISA (Cat. No E1793Ra, Cat. No E1799Ra; BT LAB, Shanghai, China) according to the manufacturer’s instructions.

Techniques:

Journal: Pharmacological Reports

Article Title: Maternal feeding patterns affect the offspring’s brain: focus on serotonin 5-HT 2C and 5-HT 2A receptors

doi: 10.1007/s43440-021-00298-0

Figure Lengend Snippet: The levels of the 5-HT 2A receptor in the prefrontal cortex, nucleus accumbens, dorsal striatum and hippocampus of male and female offspring whose mothers were fed a high-fat diet (HFD) or standard diet (SD) at postnatal day (PND) 28. n = 8 rats/group for the prefrontal cortex and nucleus accumbens; n = 7 rats/group for the dorsal striatum and hippocampus. Significance was determined using two-way ANOVA followed Tukey’s post hoc test. * p < 0.05 versus the corresponding SD group

Article Snippet: The concentrations of 5-HT 2C and 5-HT 2A receptors in the selected brain structures were measured using ELISA (Cat. No E1793Ra, Cat. No E1799Ra; BT LAB, Shanghai, China) according to the manufacturer’s instructions.

Techniques:

Journal: Pharmacological Reports

Article Title: Maternal feeding patterns affect the offspring’s brain: focus on serotonin 5-HT 2C and 5-HT 2A receptors

doi: 10.1007/s43440-021-00298-0

Figure Lengend Snippet: The levels of the 5-HT 2C receptor in the prefrontal cortex, nucleus accumbens, dorsal striatum and hippocampus of male and female offspring whose mothers were fed a high-fat diet (HFD), high-carbohydrate diet (HCD), mixed diet (MD) or standard diet (SD) at postnatal day (PND) 63. n = 8 rats/group for the prefrontal cortex and nucleus accumbens; n = 7 rats/group for the dorsal striatum and hippocampus. Significance was determined using two-way ANOVA followed Tukey’s post hoc test. ** p < 0.01, *** p < 0.001 versus the corresponding SD group

Article Snippet: The concentrations of 5-HT 2C and 5-HT 2A receptors in the selected brain structures were measured using ELISA (Cat. No E1793Ra, Cat. No E1799Ra; BT LAB, Shanghai, China) according to the manufacturer’s instructions.

Techniques:

Journal: Pharmacological Reports

Article Title: Maternal feeding patterns affect the offspring’s brain: focus on serotonin 5-HT 2C and 5-HT 2A receptors

doi: 10.1007/s43440-021-00298-0

Figure Lengend Snippet: The levels of the 5-HT 2A receptor in the prefrontal cortex, nucleus accumbens, dorsal striatum and hippocampus of male and female offspring whose mothers were fed a high-fat diet (HFD) or standard diet (SD) at postnatal day (PND) 63. n = 8 rats/group for the prefrontal cortex and nucleus accumbens; n = 7 rats/group for the dorsal striatum and hippocampus. Significance was determined using two-way ANOVA followed Tukey’s post hoc test

Article Snippet: The concentrations of 5-HT 2C and 5-HT 2A receptors in the selected brain structures were measured using ELISA (Cat. No E1793Ra, Cat. No E1799Ra; BT LAB, Shanghai, China) according to the manufacturer’s instructions.

Techniques: