Journal: Brain research

Article Title: In vitro and in vivo interactions between glioma and marrow-isolated adult multilineage inducible (MIAMI) cells.

doi: 10.1016/j.brainres.2012.07.030

Figure Lengend Snippet: Fig. 1 – Direct co-culture assay of MIAMI cells and aneuploid glioma cell lines. The DNA contents of glioma cells and MIAMI cells were analyzed by flow cytometry and the PI of aneuploid cells alone or co-cultured with MIAMI cells was calculated. (A) Example of flow cytometry histograms showing the DNA content of a co-culture of MIAMI cells (DI¼1) with aneuploid glioma cells (HS683, DI¼1.79). (B) Bar graph showing the PI of aneuploid cells alone or co-cultured with MIAMI cells. The proliferation of A172 and U118MG cell lines co-cultured for 72 h with MIAMI cells was significantly higher, and the proliferation of Lab2 and U138MG cell lines was significantly lower than control cultures. No significant differences were observed for the HS683 and Lab1 cell lines (mean7SEM from triplicate wells). n, Po0.05 versus glioma cells alone.

Article Snippet: Five human glioma cell lines were obtained from the ATCC (A172, HS683, U118MG, U138MG, U87MG; LGC Promochem, Molsheim, France) and two (Lab1 and Lab2) were generated from patients undergoing surgery for GB in the Department of Neurosurgery from CHU Angers (Table 1).

Techniques: Co-culture Assay, Cytometry, Cell Culture, Co-Culture Assay, Control

Journal: Cancers

Article Title: Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas

doi: 10.3390/cancers13205113

Figure Lengend Snippet: Hypermethylation and histone modifications account for transcriptional downregulation of DIRAS-1 and DIRAS-2. ( A ) Increase in DIRAS-1 and DIRAS-2 mRNA expression in U251MG and Hs683 cells after treatment with 5-azacytidine, ( B ) after treatment with trichostatin A (TSA), and ( C ) after combinational treatment with 5-azacytidine and trichostatin A. ( D ) Increase in DIRAS-1 and DIRAS-2 promoter DNA bound to acetylated H3, indicating DIRAS-1 and DIRAS-2 promoter euchromatinization after TSA treatment in U251MG and Hs683 cells (n.s.: not significant, * p ≤ 0.05, ** p ≤ 0.002, *** p ≤ 0.001). Original figure in .

Article Snippet: U251MG and Hs683 glioblastoma cells were obtained from Cell Lines Service GmbH (Eppelheim, Germany).

Techniques: Expressing

Journal: Cancers

Article Title: Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas

doi: 10.3390/cancers13205113

Figure Lengend Snippet: Functional analyses of the role of DIRAS-1 and DIRAS-2 in human gliomas. ( A ) Western blot analysis showing DIRAS-1 and DIRAS-2 overexpression in U251MG and Hs683 cells. Detailed information about Western Blots can be found in the . ( B ) Cell proliferation after overexpression of DIRAS-1 or DIRAS-2 and ( C ) chemosensitivity to lomustin after overexpression of DIRAS-1 or DIRAS-2 in U251MG and Hs683 cells (n.s.: not significant, * p ≤ 0.05, ** p ≤ 0.002). Original figure in .

Article Snippet: U251MG and Hs683 glioblastoma cells were obtained from Cell Lines Service GmbH (Eppelheim, Germany).

Techniques: Functional Assay, Western Blot, Over Expression

Journal: Molecular Oncology

Article Title: Hyaluronic acids mediate the infiltration, migration, and M2 polarization of macrophages: evaluating metabolic molecular phenotypes in gliomas

doi: 10.1002/1878-0261.13315

Figure Lengend Snippet: HA in the infiltration, migration, and polarization of macrophages. (A) Multiplex immunofluorescence staining of CD68 (pink), CD163 (red), and DAPI (blue) on glioma whole tissue section (10× and 40×). Scale bars: 400 and 80 μm, respectively. (B) Transwell assay for migration of HMC3 in the control group and hyaluronidase group after co‐culture of HMC3 and glioma cell lines, HS683, U251, and U87. Scale bar: 100 μm. (C) Flow diagram of the co‐culture system for Transwell assay. (D) Statistical analysis of Transwell assay for migration of HMC3 after co‐culture of HMC3 and glioma cell lines, HS683, U251, and U87. Statistical analysis was performed using an unpaired Student's t ‐test. (E) Flow diagram of the co‐culture system for immunofluorescence staining. (F) Immunofluorescence staining of CD68 and CD163 in the control and hyaluronidase groups after co‐culture of HMC3 and HS683. Scale bar: 25 μm. (G). Immunofluorescence staining of CD68 and CD11c in the control and hyaluronidase groups after co‐culture of HMC3 and HS683. Scale bar: 25 μm. (H). Statistical analysis of immunofluorescence staining of CD68 and CD163. Statistical analysis was performed using an unpaired Student's t ‐test. (I). Statistical analysis of immunofluorescence staining of CD68 and CD11c. Statistical analysis was performed using an unpaired Student's t ‐test. Data are represented as mean ± SD. The results shown are representative of three independent experiments. NS, not statistically significant; ** P < 0.01; *** P < 0.001.

Article Snippet: HS683, U251, U87, and HMC3 cell lines were purchased from iCell ( http://www.icellbioscience.com ).

Techniques: Migration, Multiplex Assay, Immunofluorescence, Staining, Transwell Assay, Control, Co-Culture Assay

Journal: Cancer Management and Research

Article Title: The Potential Significance of the EMILIN3 Gene in Augmenting the Aggressiveness of Low-Grade Gliomas is Noteworthy

doi: 10.2147/CMAR.S463694

Figure Lengend Snippet: EMILIN3 gene function experiment in LN229. qRT-PCR transfected LN229 human glioma cells efficiently (A) . The CCK-8 experiment and live/dead staining findings demonstrated that overexpression of the EMILIN3 gene may increase LN229 cell line proliferation and vitality ( B and C ). Overexpression of the EMILIN3 gene enhances the migration, invasiveness, and colony formation of the LN229 cell line (D-F ).

Article Snippet: The LN229 and HS-683 human glioma cell line were purchased from iCell Bioscience Inc, and grown in DMEM media (Gibco, USA) with 10% foetal bovine serum (BI, USA) and 1g/mL penicillin/streptomycin (Hyclone, USA) at 37 degrees Celsius and 5% carbon dioxide.

Techniques: Quantitative RT-PCR, Transfection, CCK-8 Assay, Staining, Over Expression, Migration

Journal: Aging (Albany NY)

Article Title: A three-lncRNA signature predicts clinical outcomes in low-grade glioma patients after radiotherapy

doi: 10.18632/aging.103189

Figure Lengend Snippet: Downregulation of LINC01447 or AC106786.1 enhanced radiosensitivity in low-grade glioma cells. ( A ) Representative fluorescence microscope images of HS683 cells treated with FITC-siRNA-LINC01447 or FITC-siRNA-AC106786.1: blue = DAPI; green = FITC-siRNA. ( B ) Representative flow cytometry histograms showing cellular uptake of FITC-siRNA-LINC01447 (orange), FITC-siRNA-AC106786.1 (blue), and NC-siRNA (red). ( C ) Silencing efficiency was evaluated using real-time PCR. ( D ) CCK8 assays were used to investigate the roles of LINC01447 and AC106786.1 in HS683 cell proliferation after irradiation. ( E , F ) Apoptotic cells were detected by Annexin V-FITC and PI staining. Apoptosis ratio was calculated by adding early and late apoptosis percentages. ( G ) Colony formation efficiency was used to evaluate the radiosensitivity of treated HS683 cells. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Low-grade glioma HS683 cells were purchased from Shanghai Genechem Co., Ltd. HS683 cells were cultured in DMEM (Invitrogen, Carlsbad, CA, USA) with 10% fetal bovine serum (HyClone, Logan, UT, USA).

Techniques: Fluorescence, Microscopy, Flow Cytometry, Real-time Polymerase Chain Reaction, Irradiation, Staining