hrp conjugate Funakoshi Search Results


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  • 95
    Qiagen penta his hrp conjugate kit
    Penta His Hrp Conjugate Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 79 article reviews
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    95
    Millipore monoclonal anti flag m2 peroxidase hrp antibody
    Monoclonal Anti Flag M2 Peroxidase Hrp Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Funakoshi hrp conjugate
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Hrp Conjugate, supplied by Funakoshi, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher streptavidin hrp conjugate
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Streptavidin Hrp Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 610 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    GE Healthcare hrp conjugated donkey antirabbit igg
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Hrp Conjugated Donkey Antirabbit Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    TaKaRa hrp conjugated streptavidin
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Hrp Conjugated Streptavidin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    GE Healthcare hrp conjugated anti mouse
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Hrp Conjugated Anti Mouse, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 988 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Nichirei horseradish peroxidase hrp conjugated streptavidin
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Horseradish Peroxidase Hrp Conjugated Streptavidin, supplied by Nichirei, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Funakoshi horseradish peroxidase hrp conjugated streptavidin solution
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Horseradish Peroxidase Hrp Conjugated Streptavidin Solution, supplied by Funakoshi, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    GE Healthcare hrp conjugated goat anti rabbit igg
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Hrp Conjugated Goat Anti Rabbit Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Vector Laboratories vectastain abc kit
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Vectastain Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 21111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies horseradish peroxidase hrp conjugated rabbit anti mouse igg
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Horseradish Peroxidase Hrp Conjugated Rabbit Anti Mouse Igg, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    GE Healthcare hrp conjugated goat anti mouse igg
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Hrp Conjugated Goat Anti Mouse Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Cell Signaling Technology Inc β actin
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 30584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher mouse monoclonal anti v5 hrp
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Mouse Monoclonal Anti V5 Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Bio-Rad hrp conjugated goat anti human igg
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Hrp Conjugated Goat Anti Human Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Funakoshi streptavidin
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Streptavidin, supplied by Funakoshi, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Funakoshi anti ha antibody
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Anti Ha Antibody, supplied by Funakoshi, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Funakoshi blockace
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Blockace, supplied by Funakoshi, used in various techniques. Bioz Stars score: 96/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Funakoshi phosphospecific pparγ ser273
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    Millipore streptavidin agarose beads
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
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    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
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    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
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    Disruption of cell-cell adhesion and induction of cell invasion after <t>β-estradiol</t> stimulation was mediated by NO and c-Src. A , MCF7 cells were stimulated with β-estradiol (100 n m ) in the presence or absence of either <t>L-NMMA</t> (50 μ
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    Disruption of cell-cell adhesion and induction of cell invasion after <t>β-estradiol</t> stimulation was mediated by NO and c-Src. A , MCF7 cells were stimulated with β-estradiol (100 n m ) in the presence or absence of either <t>L-NMMA</t> (50 μ
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    Disruption of cell-cell adhesion and induction of cell invasion after <t>β-estradiol</t> stimulation was mediated by NO and c-Src. A , MCF7 cells were stimulated with β-estradiol (100 n m ) in the presence or absence of either <t>L-NMMA</t> (50 μ
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    (a) Binding ability of DHS to PPAR in a nuclear receptor cofactor assay. GW1929 was used as a positive control. This experiment was performed in duplicate, and the average values are shown. (b) Transactivation activity of the <t>PPARγ-derived</t> reporter gene in HepG2 cells after treatment with troglitazone (T) and DHS (D) in μM. Relative luciferase activities were normalized to β-galactosidase activity. n = 3; error bar = SD. ** p
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    Image Search Results


    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by HRP-streptavidin. (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.

    Journal: Biochemistry and Biophysics Reports

    Article Title: A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite

    doi: 10.1016/j.bbrep.2016.06.020

    Figure Lengend Snippet: Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by HRP-streptavidin. (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.

    Article Snippet: Streptavidin, HRP conjugate was purchased from Funakoshi (Tokyo, Japan).

    Techniques: Modification, Incubation

    CuAAC reaction of DPE with an azide-linked tag molecule. (A) Formation of 1,2,3-triazole by CuAAC reaction with DPE and benzyl azide. (B) Detection of GAPDH tagged by CuAAC reaction with DPE and the azide-labeled biotin. GAPDH (1 mg/ml) was incubated with or without 50 µM DPE in the presence or absence of 30 U laccase in 70 mM sodium phosphate buffer (pH 7.2) for 1 h at 37 °C. DPE-tagged GAPDH was detected by CBB staining (left) and HRP-streptavidin (right).

    Journal: Biochemistry and Biophysics Reports

    Article Title: A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite

    doi: 10.1016/j.bbrep.2016.06.020

    Figure Lengend Snippet: CuAAC reaction of DPE with an azide-linked tag molecule. (A) Formation of 1,2,3-triazole by CuAAC reaction with DPE and benzyl azide. (B) Detection of GAPDH tagged by CuAAC reaction with DPE and the azide-labeled biotin. GAPDH (1 mg/ml) was incubated with or without 50 µM DPE in the presence or absence of 30 U laccase in 70 mM sodium phosphate buffer (pH 7.2) for 1 h at 37 °C. DPE-tagged GAPDH was detected by CBB staining (left) and HRP-streptavidin (right).

    Article Snippet: Streptavidin, HRP conjugate was purchased from Funakoshi (Tokyo, Japan).

    Techniques: Labeling, Incubation, Staining

    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by HRP-streptavidin. (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.

    Journal: Biochemistry and Biophysics Reports

    Article Title: A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite

    doi: 10.1016/j.bbrep.2016.06.020

    Figure Lengend Snippet: Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by HRP-streptavidin. (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.

    Article Snippet: Streptavidin, HRP conjugate was purchased from Funakoshi (Tokyo, Japan).

    Techniques: Modification, Incubation

    CuAAC reaction of DPE with an azide-linked tag molecule. (A) Formation of 1,2,3-triazole by CuAAC reaction with DPE and benzyl azide. (B) Detection of GAPDH tagged by CuAAC reaction with DPE and the azide-labeled biotin. GAPDH (1 mg/ml) was incubated with or without 50 µM DPE in the presence or absence of 30 U laccase in 70 mM sodium phosphate buffer (pH 7.2) for 1 h at 37 °C. DPE-tagged GAPDH was detected by CBB staining (left) and HRP-streptavidin (right).

    Journal: Biochemistry and Biophysics Reports

    Article Title: A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite

    doi: 10.1016/j.bbrep.2016.06.020

    Figure Lengend Snippet: CuAAC reaction of DPE with an azide-linked tag molecule. (A) Formation of 1,2,3-triazole by CuAAC reaction with DPE and benzyl azide. (B) Detection of GAPDH tagged by CuAAC reaction with DPE and the azide-labeled biotin. GAPDH (1 mg/ml) was incubated with or without 50 µM DPE in the presence or absence of 30 U laccase in 70 mM sodium phosphate buffer (pH 7.2) for 1 h at 37 °C. DPE-tagged GAPDH was detected by CBB staining (left) and HRP-streptavidin (right).

    Article Snippet: Streptavidin, HRP conjugate was purchased from Funakoshi (Tokyo, Japan).

    Techniques: Labeling, Incubation, Staining

    Identification of the target proteins of DOPAC. Detection of the DPE-modified Keap1 and AhR. Confluent Hepa1c1c7 cells were incubated with 50 µM DPE for 5 h in serum-free MEM-α. The cell lysate was incubated with Streptavidin Mag Sepharose beads for 30 min. The DPE-modified Keap1 (left) or AhR (right) were detected by immunoblot analysis.

    Journal: Biochemistry and Biophysics Reports

    Article Title: A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite

    doi: 10.1016/j.bbrep.2016.06.020

    Figure Lengend Snippet: Identification of the target proteins of DOPAC. Detection of the DPE-modified Keap1 and AhR. Confluent Hepa1c1c7 cells were incubated with 50 µM DPE for 5 h in serum-free MEM-α. The cell lysate was incubated with Streptavidin Mag Sepharose beads for 30 min. The DPE-modified Keap1 (left) or AhR (right) were detected by immunoblot analysis.

    Article Snippet: Streptavidin, HRP conjugate was purchased from Funakoshi (Tokyo, Japan).

    Techniques: Modification, Incubation

    Disruption of cell-cell adhesion and induction of cell invasion after β-estradiol stimulation was mediated by NO and c-Src. A , MCF7 cells were stimulated with β-estradiol (100 n m ) in the presence or absence of either L-NMMA (50 μ

    Journal: The Journal of Biological Chemistry

    Article Title: S-Nitrosylation at Cysteine 498 of c-Src Tyrosine Kinase Regulates Nitric Oxide-mediated Cell Invasion *

    doi: 10.1074/jbc.M109.059782

    Figure Lengend Snippet: Disruption of cell-cell adhesion and induction of cell invasion after β-estradiol stimulation was mediated by NO and c-Src. A , MCF7 cells were stimulated with β-estradiol (100 n m ) in the presence or absence of either L-NMMA (50 μ

    Article Snippet: Other reagents were obtained from the following manufacturers: β-estrogen, S -methyl methanethiosulfonate (MMTS), biotin HRP, PP2, and L-NMMA (Funakoshi, Tokyo, Japan); HRP-conjugated streptavidin (Takara, Tokyo, Japan); streptavidin-agarose beads (Sigma); Micro Bio-Spin 6 column (Bio-Rad); nickel-nitrilotriacetic acid (Ni-NTA; Qiagen).

    Techniques:

    (a) Binding ability of DHS to PPAR in a nuclear receptor cofactor assay. GW1929 was used as a positive control. This experiment was performed in duplicate, and the average values are shown. (b) Transactivation activity of the PPARγ-derived reporter gene in HepG2 cells after treatment with troglitazone (T) and DHS (D) in μM. Relative luciferase activities were normalized to β-galactosidase activity. n = 3; error bar = SD. ** p

    Journal: ACS Omega

    Article Title: Dihydrosanguinarine Enhances Glucose Uptake in Mouse 3T3-L1 Cells

    doi: 10.1021/acsomega.7b01134

    Figure Lengend Snippet: (a) Binding ability of DHS to PPAR in a nuclear receptor cofactor assay. GW1929 was used as a positive control. This experiment was performed in duplicate, and the average values are shown. (b) Transactivation activity of the PPARγ-derived reporter gene in HepG2 cells after treatment with troglitazone (T) and DHS (D) in μM. Relative luciferase activities were normalized to β-galactosidase activity. n = 3; error bar = SD. ** p

    Article Snippet: Target-specific antibodies were obtained from the following manufacturers: AMPKα, C/EBPα, PPARγ, and β-actin from Cell Signaling Technology (Beverly, MA, U.S.A.); phosphospecific PPARγ (Ser273) from Funakoshi Co., Ltd. (Japan); and HRP-conjugated donkey antirabbit IgG from GE Healthcare (Buckinghamshire, U.K.).

    Techniques: Binding Assay, Positive Control, Activity Assay, Derivative Assay, Luciferase