Journal: Brazilian Journal of Microbiology

Article Title: A fluorometric hybridization assay for detecting and genotyping high-risk human papillomavirus 16 and 18 in archival tissues of cervical specimens

doi: 10.1007/s42770-019-00137-8

Figure Lengend Snippet: HPV types 16 and 18 prevalence in patients with different types of cervical dysplasia

Article Snippet: Source of plasmids DNA DNA plasmids containing the entire genomes of HPV16 (ATCC 45113D) and HPV18 (ATCC 45152D) were purchased from American Type Culture Collection (ATCC, Manassas, USA) and used as the control to develop efficient hybridization methods.

Techniques:

Journal: Brazilian Journal of Microbiology

Article Title: A fluorometric hybridization assay for detecting and genotyping high-risk human papillomavirus 16 and 18 in archival tissues of cervical specimens

doi: 10.1007/s42770-019-00137-8

Figure Lengend Snippet: HPV types 16 and 18 overall detection via three different methods

Article Snippet: Source of plasmids DNA DNA plasmids containing the entire genomes of HPV16 (ATCC 45113D) and HPV18 (ATCC 45152D) were purchased from American Type Culture Collection (ATCC, Manassas, USA) and used as the control to develop efficient hybridization methods.

Techniques:

Journal: Brazilian Journal of Microbiology

Article Title: A fluorometric hybridization assay for detecting and genotyping high-risk human papillomavirus 16 and 18 in archival tissues of cervical specimens

doi: 10.1007/s42770-019-00137-8

Figure Lengend Snippet: Comparison between the results obtained for the identification of HPV 16 and 18, with TSnPCR and with MCHA

Article Snippet: Source of plasmids DNA DNA plasmids containing the entire genomes of HPV16 (ATCC 45113D) and HPV18 (ATCC 45152D) were purchased from American Type Culture Collection (ATCC, Manassas, USA) and used as the control to develop efficient hybridization methods.

Techniques: Comparison

Journal: Brazilian Journal of Microbiology

Article Title: A fluorometric hybridization assay for detecting and genotyping high-risk human papillomavirus 16 and 18 in archival tissues of cervical specimens

doi: 10.1007/s42770-019-00137-8

Figure Lengend Snippet: Comparison of the results obtained for the identification of HPV 16 and 18, with TSnPCR and with MFHA

Article Snippet: Source of plasmids DNA DNA plasmids containing the entire genomes of HPV16 (ATCC 45113D) and HPV18 (ATCC 45152D) were purchased from American Type Culture Collection (ATCC, Manassas, USA) and used as the control to develop efficient hybridization methods.

Techniques: Comparison

Journal: Frontiers in Oncology

Article Title: Loop-Mediated Isothermal Amplification Assay for Detecting Tumor Markers and Human Papillomavirus: Accuracy and Supplemental Diagnostic Value to Endovaginal MRI in Cervical Cancer

doi: 10.3389/fonc.2021.747614

Figure Lengend Snippet: Limits of detection and time to positive for synthetic sequences and clinical samples in Groups 1 and 2 of tumor markers and HPV 16 and 18.

Article Snippet: LAMP-HPV 18 DNA and/or mRNA , 38 , GenoID and Norchip test , 100.0 (80.6, 100) , 22.2 (7.3, 38.5) , 47.1 (31.5, 63.3) , 100.0 (51.0, 100).

Techniques: Marker, Sequencing

Journal: Frontiers in Oncology

Article Title: Loop-Mediated Isothermal Amplification Assay for Detecting Tumor Markers and Human Papillomavirus: Accuracy and Supplemental Diagnostic Value to Endovaginal MRI in Cervical Cancer

doi: 10.3389/fonc.2021.747614

Figure Lengend Snippet: Sensitivity, specificity, positive and negative predictive values of LAMP assays for detection of small volume Stage 1 cervical cancer alone and together with endovaginal MRI.

Article Snippet: LAMP-HPV 18 DNA and/or mRNA , 38 , GenoID and Norchip test , 100.0 (80.6, 100) , 22.2 (7.3, 38.5) , 47.1 (31.5, 63.3) , 100.0 (51.0, 100).

Techniques: Histopathology, Marker

Journal: Vaccines

Article Title: Human Papillomavirus-Related Cancer Vaccine Strategies

doi: 10.3390/vaccines12111291

Figure Lengend Snippet: Prophylactic HPV vaccines that have been approved for marketing.

Article Snippet: , VB10.16 , Phase II , L1 proteins of HPV 16 and 18 , VBI Vaccines, Inc., Cambridge, MA, USA.

Techniques: Vaccines, Produced, Expressing, Adjuvant, Injection, Infection

Journal: Vaccines

Article Title: Human Papillomavirus-Related Cancer Vaccine Strategies

doi: 10.3390/vaccines12111291

Figure Lengend Snippet: Globally in-clinical-stage prophylactic and therapeutic HPV vaccines.

Article Snippet: , VB10.16 , Phase II , L1 proteins of HPV 16 and 18 , VBI Vaccines, Inc., Cambridge, MA, USA.

Techniques: Vaccines, Clinical Proteomics

Journal: Frontiers in Oncology

Article Title: Putative Role of Circulating Human Papillomavirus DNA in the Development of Primary Squamous Cell Carcinoma of the Middle Rectum: A Case Report

doi: 10.3389/fonc.2019.00093

Figure Lengend Snippet: Synoptical table of the methods applied in the different samples and the respective results obtained.

Article Snippet: Therefore, we searched the virus in the tumor mass, in its margins, in tumor-free normal rectal mucosa proximal to the tumor and in the lymph nodes by IHC for p16, PCR assays, CISH for HPV nucleic acids (ZytoFast PLUS CISH Implementation Kit HRP-DAB and ZytoFast HPV type 16/18 Probe, ZytoVision, Bio-Optica, Milan, Italy) and RNA ISH for E6/E7 mRNA transcripts (RNAscope®2.5 HD Assay- RED, probe HPV 16, Acdbio, Milan, Italy) ( ).

Techniques: RNAscope, Digital PCR, Marker, Double Staining, Clinical Proteomics, In Vitro, Transfection

Journal: Frontiers in Oncology

Article Title: Putative Role of Circulating Human Papillomavirus DNA in the Development of Primary Squamous Cell Carcinoma of the Middle Rectum: A Case Report

doi: 10.3389/fonc.2019.00093

Figure Lengend Snippet: Human Papillomavirus polimerase chain reaction identification and typing. The presence of HPV 16 DNA was confirmed by HPV 16 genotype specific primer pair 5′- AAAGCCACTGTGTCCTGAAGA-3′ and 5′-CTGGGTTTCTCTACGTGTTCT-3′ able to amplify a 130 bps long fragment (424–553 nt, ref Seq Human Papillomavirus 16 type NC_001526). B, Blank, (1) Tumor mass, (2) Proximal margin, (3) Distal margin, (4) Lymph node, (5) Tumor-free mucosa of the surgical specimen, (6) Descending colon biopsies, (7) Caecum/ascending colon biopsies, (8) HPV DNA negative cervical cytological specimen, (9) HPV DNA positive cervical cytological specimen, M, Molecular weight marker (A) . HPV 16 was present in the tumor mass as it is evident by the sequence alignment of the 460 bps L1 sequence obtained by MY11/09 PCR primers (B) . HPV, Human Papillomavirus.

Article Snippet: Therefore, we searched the virus in the tumor mass, in its margins, in tumor-free normal rectal mucosa proximal to the tumor and in the lymph nodes by IHC for p16, PCR assays, CISH for HPV nucleic acids (ZytoFast PLUS CISH Implementation Kit HRP-DAB and ZytoFast HPV type 16/18 Probe, ZytoVision, Bio-Optica, Milan, Italy) and RNA ISH for E6/E7 mRNA transcripts (RNAscope®2.5 HD Assay- RED, probe HPV 16, Acdbio, Milan, Italy) ( ).

Techniques: Molecular Weight, Marker, Sequencing

Journal: Frontiers in Oncology

Article Title: Putative Role of Circulating Human Papillomavirus DNA in the Development of Primary Squamous Cell Carcinoma of the Middle Rectum: A Case Report

doi: 10.3389/fonc.2019.00093

Figure Lengend Snippet: Human papillomavirus circulation. Electrophoresis showed the amplified HPV DNA obtained from plasma and Caski cells supernatant-derived exosomes. B, Blank; (1) Plasma-derived exosomes; (2) Caski cells; (3) Positive control (cervical HPV DNA positive cytological samples); M, Marker. The PCR assay was performed with MY11/09 primers (A) . Nucleotide blast of HPV DNA amplified product obtained from plasma-derived exosomes by MY11/09 primers (B) . HCT116 and NCM460 cell lines were exposed to CasKi supernatant derived exosomes and analyzed by Digital PCR (QuantStudio® 3D Digital PCR System, Life Technologies) to determine the capability of exosomes to transfer viral genetic material to recipient cells. Caski cell line was established from a metastasis in the small bowel mesentery and contains HPV16 DNA integrated into the genome. The reaction was performed using TaqMan Assay specific for HPV 16 E1 (Vi03453396_s1, Invitrogen, Milan, Italy). After 6 h of exposure the load of HPV DNA was greater than at 20 h in the normal human colon epithelial cell line (NCM460). In the human colon cancer cell line (HCT116) we report an HPV DNA burst at 6 h and a rapid decay, indeed it was absent at 20 h post exposure. (1) HCT116 at 3 h; (2) HCT116 at 6 h; (3) HCT116 at 20 h; (4) NCM460 at 3 h; (5) NCM460 at 6 h; (6) NCM4 60 at 20 h (C) . The specimens illustrated in (C) were also amplified by conventional PCR with E6 HPV 16 specific primer pair showing concordant results with Digital PCR. B, Blank, (1) not exposed HCT116; (2) HCT116 at 3 h; (3) HCT116 at 6 h; (4) HCT116 at 20 h; (5) not exposed NCM460; (6) NCM460 at 3 h; (7) NCM460 at 6 h; (8) NCM460 at 20 h; (9) blank; (10) HPV DNA negative cervical specimen; (11) HPV DNA positive cervical specimen (D) . The neoplastic and the neighbor cells demonstrated HPV DNA in the endothelial cells (red arrow) and in the fibroblasts (black arrow) by both CISH (brown chromogen) (E) and p16 IHC ( E , inset). E, (E) -inset, original magnification 20x.

Article Snippet: Therefore, we searched the virus in the tumor mass, in its margins, in tumor-free normal rectal mucosa proximal to the tumor and in the lymph nodes by IHC for p16, PCR assays, CISH for HPV nucleic acids (ZytoFast PLUS CISH Implementation Kit HRP-DAB and ZytoFast HPV type 16/18 Probe, ZytoVision, Bio-Optica, Milan, Italy) and RNA ISH for E6/E7 mRNA transcripts (RNAscope®2.5 HD Assay- RED, probe HPV 16, Acdbio, Milan, Italy) ( ).

Techniques: Electrophoresis, Amplification, Clinical Proteomics, Derivative Assay, Positive Control, Marker, Digital PCR, TaqMan Assay