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  • 90
    Thermo Fisher hprt1 hs02800695
    Hprt1 Hs02800695, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore hprt1
    Hprt1, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hprt1 mm01545399
    Hprt1 Mm01545399, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hprt 1
    uPAR is necessary for hypoxia-promoted cell migration, invasion, and Rac1 activation. (A) MDA-MB-468 cells were pretreated with 25 μg/ml uPA-specific antibody 3471 and 25 μg/ml uPAR-specific antibody 399R or with 50 μg/ml control IgG. Cells were allowed to migrate in Transwell chambers for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). Cell migration is expressed as a percentage of that observed with control IgG in normoxia (mean ± SEM; n = 6). (B) MDA-MB-468 cells expressing empty vector (pSUPER), sh-uPAR6 cells, and sh-uPAR12 cells were cultured for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). uPAR mRNA was determined by qPCR. Results are normalized against <t>HPRT-1</t> and compared with the level observed in the pSUPER cells in normoxia (mean ± SEM; n = 3). (C) pSUPER, sh-uPAR6, and sh-uPAR12 cells were allowed to migrate in Transwell chamber (cell migration) or to invade Matrigel (cell invasion) for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). Results are expressed as a percentage of that observed with pSUPER cells in normoxia (mean ± SEM; n = 7 and n = 6, respectively). (D) pSUPER, sh-uPAR6, and sh-uPAR12 cells were cultured for 15 h in 21% O 2 (N) or in 1.0% O 2 (H). Cell extracts were affinity precipitated with PAK-1 PBD and subjected to immunoblot analysis to determine GTP-bound Rac1. The original extracts were subjected to immunoblot analysis for tubulin, as a loading control.
    Hprt 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche hprt 1
    uPAR is necessary for hypoxia-promoted cell migration, invasion, and Rac1 activation. (A) MDA-MB-468 cells were pretreated with 25 μg/ml uPA-specific antibody 3471 and 25 μg/ml uPAR-specific antibody 399R or with 50 μg/ml control IgG. Cells were allowed to migrate in Transwell chambers for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). Cell migration is expressed as a percentage of that observed with control IgG in normoxia (mean ± SEM; n = 6). (B) MDA-MB-468 cells expressing empty vector (pSUPER), sh-uPAR6 cells, and sh-uPAR12 cells were cultured for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). uPAR mRNA was determined by qPCR. Results are normalized against <t>HPRT-1</t> and compared with the level observed in the pSUPER cells in normoxia (mean ± SEM; n = 3). (C) pSUPER, sh-uPAR6, and sh-uPAR12 cells were allowed to migrate in Transwell chamber (cell migration) or to invade Matrigel (cell invasion) for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). Results are expressed as a percentage of that observed with pSUPER cells in normoxia (mean ± SEM; n = 7 and n = 6, respectively). (D) pSUPER, sh-uPAR6, and sh-uPAR12 cells were cultured for 15 h in 21% O 2 (N) or in 1.0% O 2 (H). Cell extracts were affinity precipitated with PAK-1 PBD and subjected to immunoblot analysis to determine GTP-bound Rac1. The original extracts were subjected to immunoblot analysis for tubulin, as a loading control.
    Hprt 1, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SABiosciences hprt 1
    uPAR is necessary for hypoxia-promoted cell migration, invasion, and Rac1 activation. (A) MDA-MB-468 cells were pretreated with 25 μg/ml uPA-specific antibody 3471 and 25 μg/ml uPAR-specific antibody 399R or with 50 μg/ml control IgG. Cells were allowed to migrate in Transwell chambers for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). Cell migration is expressed as a percentage of that observed with control IgG in normoxia (mean ± SEM; n = 6). (B) MDA-MB-468 cells expressing empty vector (pSUPER), sh-uPAR6 cells, and sh-uPAR12 cells were cultured for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). uPAR mRNA was determined by qPCR. Results are normalized against <t>HPRT-1</t> and compared with the level observed in the pSUPER cells in normoxia (mean ± SEM; n = 3). (C) pSUPER, sh-uPAR6, and sh-uPAR12 cells were allowed to migrate in Transwell chamber (cell migration) or to invade Matrigel (cell invasion) for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). Results are expressed as a percentage of that observed with pSUPER cells in normoxia (mean ± SEM; n = 7 and n = 6, respectively). (D) pSUPER, sh-uPAR6, and sh-uPAR12 cells were cultured for 15 h in 21% O 2 (N) or in 1.0% O 2 (H). Cell extracts were affinity precipitated with PAK-1 PBD and subjected to immunoblot analysis to determine GTP-bound Rac1. The original extracts were subjected to immunoblot analysis for tubulin, as a loading control.
    Hprt 1, supplied by SABiosciences, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech hprt1 message
    IUGR+HFD female rats had decreased serum HDL (A) compared to Con+HFD female rats, increased hepatic triglycerides (B) and cholesterol (C) , decreased hepatic bile acids (C) , decreased Cyp7a1 protein, and increased miR-122 (D) compared to Con+Reg female rats. A maternal HFD increased cholesterol and hepatic Cyp7a1 mRNA and protein in both sexes in IUGR and control rats (D) . Data shown as scatter plots of individual rats with mean ± SD with a minimum n = 6 rats per sex, per intrauterine environment, each from separate litters. Data from male rats is shown on the left of the figure, and from female rats is shown on the right. Groups are denoted as follows: Con+Reg data are shown in black, Con+HFD data are shown in yellow, IUGR+Reg data are shown in blue, and IUGR+HFD data are shown in red, with group names listed below the graphs on the bottom of the figure. Western blot images of Lxrα, Cyp7a1, and <t>Hprt1</t> protein are shown above the graphical representation of band densitometry. The kilodalton (kDa) marker is shown on the left lane of the western blot image; the 50 kDa marker is shown in the Lxrα blot, the 60 kDa marker is shown in the Cyp7a1 blot, and the 30 kDa marker is shown in the Hprt1 blot and the Hprt1 image was obtained from the blot with Cyp7a1. A p -value ≤0.05 is denoted with an asterisk ( ∗ ) for any group data compared to sex-matched Con+Reg, and a p -value ≤ 0.05 is denoted with a hatched line (†) for IUGR+HFD data compared to sex-matched Con+HFD.
    Hprt1 Message, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SABiosciences hprt 1 primers
    IUGR+HFD female rats had decreased serum HDL (A) compared to Con+HFD female rats, increased hepatic triglycerides (B) and cholesterol (C) , decreased hepatic bile acids (C) , decreased Cyp7a1 protein, and increased miR-122 (D) compared to Con+Reg female rats. A maternal HFD increased cholesterol and hepatic Cyp7a1 mRNA and protein in both sexes in IUGR and control rats (D) . Data shown as scatter plots of individual rats with mean ± SD with a minimum n = 6 rats per sex, per intrauterine environment, each from separate litters. Data from male rats is shown on the left of the figure, and from female rats is shown on the right. Groups are denoted as follows: Con+Reg data are shown in black, Con+HFD data are shown in yellow, IUGR+Reg data are shown in blue, and IUGR+HFD data are shown in red, with group names listed below the graphs on the bottom of the figure. Western blot images of Lxrα, Cyp7a1, and <t>Hprt1</t> protein are shown above the graphical representation of band densitometry. The kilodalton (kDa) marker is shown on the left lane of the western blot image; the 50 kDa marker is shown in the Lxrα blot, the 60 kDa marker is shown in the Cyp7a1 blot, and the 30 kDa marker is shown in the Hprt1 blot and the Hprt1 image was obtained from the blot with Cyp7a1. A p -value ≤0.05 is denoted with an asterisk ( ∗ ) for any group data compared to sex-matched Con+Reg, and a p -value ≤ 0.05 is denoted with a hatched line (†) for IUGR+HFD data compared to sex-matched Con+HFD.
    Hprt 1 Primers, supplied by SABiosciences, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher gene exp hprt1 ss03388274 m1
    IUGR+HFD female rats had decreased serum HDL (A) compared to Con+HFD female rats, increased hepatic triglycerides (B) and cholesterol (C) , decreased hepatic bile acids (C) , decreased Cyp7a1 protein, and increased miR-122 (D) compared to Con+Reg female rats. A maternal HFD increased cholesterol and hepatic Cyp7a1 mRNA and protein in both sexes in IUGR and control rats (D) . Data shown as scatter plots of individual rats with mean ± SD with a minimum n = 6 rats per sex, per intrauterine environment, each from separate litters. Data from male rats is shown on the left of the figure, and from female rats is shown on the right. Groups are denoted as follows: Con+Reg data are shown in black, Con+HFD data are shown in yellow, IUGR+Reg data are shown in blue, and IUGR+HFD data are shown in red, with group names listed below the graphs on the bottom of the figure. Western blot images of Lxrα, Cyp7a1, and <t>Hprt1</t> protein are shown above the graphical representation of band densitometry. The kilodalton (kDa) marker is shown on the left lane of the western blot image; the 50 kDa marker is shown in the Lxrα blot, the 60 kDa marker is shown in the Cyp7a1 blot, and the 30 kDa marker is shown in the Hprt1 blot and the Hprt1 image was obtained from the blot with Cyp7a1. A p -value ≤0.05 is denoted with an asterisk ( ∗ ) for any group data compared to sex-matched Con+Reg, and a p -value ≤ 0.05 is denoted with a hatched line (†) for IUGR+HFD data compared to sex-matched Con+HFD.
    Gene Exp Hprt1 Ss03388274 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TIB MOLBIOL hprt1 taqman probe
    IUGR+HFD female rats had decreased serum HDL (A) compared to Con+HFD female rats, increased hepatic triglycerides (B) and cholesterol (C) , decreased hepatic bile acids (C) , decreased Cyp7a1 protein, and increased miR-122 (D) compared to Con+Reg female rats. A maternal HFD increased cholesterol and hepatic Cyp7a1 mRNA and protein in both sexes in IUGR and control rats (D) . Data shown as scatter plots of individual rats with mean ± SD with a minimum n = 6 rats per sex, per intrauterine environment, each from separate litters. Data from male rats is shown on the left of the figure, and from female rats is shown on the right. Groups are denoted as follows: Con+Reg data are shown in black, Con+HFD data are shown in yellow, IUGR+Reg data are shown in blue, and IUGR+HFD data are shown in red, with group names listed below the graphs on the bottom of the figure. Western blot images of Lxrα, Cyp7a1, and <t>Hprt1</t> protein are shown above the graphical representation of band densitometry. The kilodalton (kDa) marker is shown on the left lane of the western blot image; the 50 kDa marker is shown in the Lxrα blot, the 60 kDa marker is shown in the Cyp7a1 blot, and the 30 kDa marker is shown in the Hprt1 blot and the Hprt1 image was obtained from the blot with Cyp7a1. A p -value ≤0.05 is denoted with an asterisk ( ∗ ) for any group data compared to sex-matched Con+Reg, and a p -value ≤ 0.05 is denoted with a hatched line (†) for IUGR+HFD data compared to sex-matched Con+HFD.
    Hprt1 Taqman Probe, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp hprt1 hs99999909 m1
    Expression of COPZ2 in thyroid tumor cell lines Real-time PCR analysis of COPZ2 gene expression; results are presented as log10-transformed relative quantity (RQ) of COPZ2 mRNA normalized for <t>HPRT1</t> housekeeping gene expression. Data represent the mean +/− sd of three independent experiments.
    Gene Exp Hprt1 Hs99999909 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hprt1 gene
    Expression of COPZ2 in thyroid tumor cell lines Real-time PCR analysis of COPZ2 gene expression; results are presented as log10-transformed relative quantity (RQ) of COPZ2 mRNA normalized for <t>HPRT1</t> housekeeping gene expression. Data represent the mean +/− sd of three independent experiments.
    Hprt1 Gene, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp hprt1 hs01003267 m1
    Effect of rs1790834 genotypes on expression of CYB5A and steroidogenic genes. Real-time quantitative PCR analysis of mRNA expression in 22 RA synovial fibroblast lines (rs1790834 genotype GG, n = 15; AA/AG genotypes, n = 7). (A) Synovial fibroblasts express all genes, which are necessary for synthesis of biologically active androgens. Mean ± SD of expression levels normalized to <t>HPRT1</t> of combined data for all genotypes. (B) rs1790834 genotype-dependent expression levels, which are normalized to the median value of the combined expression data for each gene. In the box-and-whisker plots the genotypes with the rare allele A were grouped together (AA, n = 1; AG, n = 6). The boxes represent the 25th to 75th percentiles, and horizontal lines within the box represent median values. The whiskers show the 10th and 90th percentiles, respectively, and dots represent individual values exceeding these limits. ***Significant rs1790834 genotype-dependent expression, P
    Gene Exp Hprt1 Hs01003267 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp hprt1 rn01527840 m1
    Sex-dependent expression of Oat1 and Oat3 in rat cortical kidney slices. Levels of β-actin, <t>Hprt1,</t> Oat1 and Oat3 were analyzed by TaqMan® real-time PCR in total RNA isolated from four male and four female cortical kidney slices. The mRNA expression of reference genes β-actin and <t>Hprt1</t> were investigated by comparing their Ct values between male and female (2A). Levels of Oat1 and Oat3 were determined using 2 −ΔΔCt method, at which β-actin was the reference gene (2B). ΔΔCt values were calculated as ΔCt male - ΔCt female. n male = 4; n female = 4.
    Gene Exp Hprt1 Rn01527840 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher human genes hprt1 hs02800695
    Sex-dependent expression of Oat1 and Oat3 in rat cortical kidney slices. Levels of β-actin, <t>Hprt1,</t> Oat1 and Oat3 were analyzed by TaqMan® real-time PCR in total RNA isolated from four male and four female cortical kidney slices. The mRNA expression of reference genes β-actin and <t>Hprt1</t> were investigated by comparing their Ct values between male and female (2A). Levels of Oat1 and Oat3 were determined using 2 −ΔΔCt method, at which β-actin was the reference gene (2B). ΔΔCt values were calculated as ΔCt male - ΔCt female. n male = 4; n female = 4.
    Human Genes Hprt1 Hs02800695, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp hprt1 hs02800695 m1
    PDGF-PDGFRβ signaling regulates key downstream signaling mediators. qPCR analyses on mRNA levels of (A) MDM2 and (B) MDM4 relative to untreated Y79 cells under the cell culture conditions described above. <t>HPRT1</t> used as housekeeping gene. Western blot analyses (C) of MDM2 activity and (D) AKT activity after disruption of the PDGFRβ signaling cascade. IM, *P = 0.0275; rhPDGF + IM, **P = 0.0011; AKT, *P = 0.0054. Evaluation of the antiapoptotic mediator (E) BCL-2 by Western blot (**P = 0.001 and ***P = 0.0002); and the proapoptotic cleaved (active) capsase-3 by ELISA (F), *P = 0.007. (G–L) Downregulation of the VEGFR signaling when PDGFRβ is inhibited. (G) VEGFA mRNA levels were measured by qPCR analysis in both Y79 cells and (H) PDX samples. (I) Both VEGFA and (J) VEGFR2 mRNA levels were measured across respective treatments as well as (K) Western blot analyses of VEGFR2 activity; *P = 0.0191. (L) Measurement of VEGFA levels in a cohort of vitreous samples from healthy controls compared to vitreous of Rb patients. ****P = 0.0001. (M–O) Assessment of the NF-κB signaling. Representing images of Y79 cells (M) labeled and analyzed for nuclear (labeled with DRAQ5) translocation of the p65 subunit of NF-κB (AlexaFluor 488 conjugated), ***P
    Gene Exp Hprt1 Hs02800695 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 838 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp hprt1 cf02626256 m1
    PDGF-PDGFRβ signaling regulates key downstream signaling mediators. qPCR analyses on mRNA levels of (A) MDM2 and (B) MDM4 relative to untreated Y79 cells under the cell culture conditions described above. <t>HPRT1</t> used as housekeeping gene. Western blot analyses (C) of MDM2 activity and (D) AKT activity after disruption of the PDGFRβ signaling cascade. IM, *P = 0.0275; rhPDGF + IM, **P = 0.0011; AKT, *P = 0.0054. Evaluation of the antiapoptotic mediator (E) BCL-2 by Western blot (**P = 0.001 and ***P = 0.0002); and the proapoptotic cleaved (active) capsase-3 by ELISA (F), *P = 0.007. (G–L) Downregulation of the VEGFR signaling when PDGFRβ is inhibited. (G) VEGFA mRNA levels were measured by qPCR analysis in both Y79 cells and (H) PDX samples. (I) Both VEGFA and (J) VEGFR2 mRNA levels were measured across respective treatments as well as (K) Western blot analyses of VEGFR2 activity; *P = 0.0191. (L) Measurement of VEGFA levels in a cohort of vitreous samples from healthy controls compared to vitreous of Rb patients. ****P = 0.0001. (M–O) Assessment of the NF-κB signaling. Representing images of Y79 cells (M) labeled and analyzed for nuclear (labeled with DRAQ5) translocation of the p65 subunit of NF-κB (AlexaFluor 488 conjugated), ***P
    Gene Exp Hprt1 Cf02626256 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp hprt1 hs03929096 g1
    Individuals with the p.Ala489Thr interferon-induced helicase C domain-containing protein 1 (IFIH1) mutant demonstrate elevated levels of interferon-stimulated genes compared with controls. An interferon score was calculated from the median fold change in RQ value for a panel of six interferon signature genes measured using quantitative polymerase chain reaction – IFI27 (Hs01086370_m1), IFI44L (Hs00199115_m1), IFIT1 (Hs00356631_g1), ISG15 (Hs00192713_m1), RSAD2 (Hs01057264_m1) and SIGLEC1 (Hs00988063_m1) – normalized to the expression levels of <t>HPRT1</t> with IFIH1-related disease, and is significantly higher than in control samples (**** P
    Gene Exp Hprt1 Hs03929096 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp hprt1 cf02690456 g1
    Individuals with the p.Ala489Thr interferon-induced helicase C domain-containing protein 1 (IFIH1) mutant demonstrate elevated levels of interferon-stimulated genes compared with controls. An interferon score was calculated from the median fold change in RQ value for a panel of six interferon signature genes measured using quantitative polymerase chain reaction – IFI27 (Hs01086370_m1), IFI44L (Hs00199115_m1), IFIT1 (Hs00356631_g1), ISG15 (Hs00192713_m1), RSAD2 (Hs01057264_m1) and SIGLEC1 (Hs00988063_m1) – normalized to the expression levels of <t>HPRT1</t> with IFIH1-related disease, and is significantly higher than in control samples (**** P
    Gene Exp Hprt1 Cf02690456 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp hprt1 cf02626258 m1
    HMGA1 real-time PCR analyses. Relative <t>HMGA1/HPRT1</t> and HMGA1/GUSB expression in native CT1258, CT1258-EGFP and CT1258-HMGA2-EGFP cells. Error bars are standard deviations. *p≤0.05 indicates a statistical significant increased expression of HMGA1 in CT1258-HMGA2-EGFP cells when compared to native CT1258 and CT1258-EGFP.
    Gene Exp Hprt1 Cf02626258 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher anti hprt1 polyclonal antibody
    HMGA1 real-time PCR analyses. Relative <t>HMGA1/HPRT1</t> and HMGA1/GUSB expression in native CT1258, CT1258-EGFP and CT1258-HMGA2-EGFP cells. Error bars are standard deviations. *p≤0.05 indicates a statistical significant increased expression of HMGA1 in CT1258-HMGA2-EGFP cells when compared to native CT1258 and CT1258-EGFP.
    Anti Hprt1 Polyclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hprt1 rrna
    HMGA1 real-time PCR analyses. Relative <t>HMGA1/HPRT1</t> and HMGA1/GUSB expression in native CT1258, CT1258-EGFP and CT1258-HMGA2-EGFP cells. Error bars are standard deviations. *p≤0.05 indicates a statistical significant increased expression of HMGA1 in CT1258-HMGA2-EGFP cells when compared to native CT1258 and CT1258-EGFP.
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    Qiagen mm hprt 1 sg
    HMGA1 real-time PCR analyses. Relative <t>HMGA1/HPRT1</t> and HMGA1/GUSB expression in native CT1258, CT1258-EGFP and CT1258-HMGA2-EGFP cells. Error bars are standard deviations. *p≤0.05 indicates a statistical significant increased expression of HMGA1 in CT1258-HMGA2-EGFP cells when compared to native CT1258 and CT1258-EGFP.
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    Hepatic protein levels of Ldlr relative to control protein <t>Hprt1.</t> * P ≤ 0.05 compared to sex‐matched Con+Reg, † P ≤ 0.05 compared to sex‐matched Con+ HFD . Data from Con+Reg rats are shown in white bars, Con+ HFD rats are shown in black bars, IUGR +Reg rats shown in light gray bars, and IUGR + HFD rats are shown in dark gray bars. Western blot image is depicted below the graph, with a representative Hprt1 control blot at the bottom. N = 6 per sex, diet, and surgical intervention. Data are shown as mean ± SEM . HFD, high‐saturated‐fat diet; IUGR, intrauterine growth restriction.
    Hprt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    uPAR is necessary for hypoxia-promoted cell migration, invasion, and Rac1 activation. (A) MDA-MB-468 cells were pretreated with 25 μg/ml uPA-specific antibody 3471 and 25 μg/ml uPAR-specific antibody 399R or with 50 μg/ml control IgG. Cells were allowed to migrate in Transwell chambers for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). Cell migration is expressed as a percentage of that observed with control IgG in normoxia (mean ± SEM; n = 6). (B) MDA-MB-468 cells expressing empty vector (pSUPER), sh-uPAR6 cells, and sh-uPAR12 cells were cultured for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). uPAR mRNA was determined by qPCR. Results are normalized against HPRT-1 and compared with the level observed in the pSUPER cells in normoxia (mean ± SEM; n = 3). (C) pSUPER, sh-uPAR6, and sh-uPAR12 cells were allowed to migrate in Transwell chamber (cell migration) or to invade Matrigel (cell invasion) for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). Results are expressed as a percentage of that observed with pSUPER cells in normoxia (mean ± SEM; n = 7 and n = 6, respectively). (D) pSUPER, sh-uPAR6, and sh-uPAR12 cells were cultured for 15 h in 21% O 2 (N) or in 1.0% O 2 (H). Cell extracts were affinity precipitated with PAK-1 PBD and subjected to immunoblot analysis to determine GTP-bound Rac1. The original extracts were subjected to immunoblot analysis for tubulin, as a loading control.

    Journal: The Journal of Cell Biology

    Article Title: uPAR induces epithelial-mesenchymal transition in hypoxic breast cancer cells

    doi: 10.1083/jcb.200701092

    Figure Lengend Snippet: uPAR is necessary for hypoxia-promoted cell migration, invasion, and Rac1 activation. (A) MDA-MB-468 cells were pretreated with 25 μg/ml uPA-specific antibody 3471 and 25 μg/ml uPAR-specific antibody 399R or with 50 μg/ml control IgG. Cells were allowed to migrate in Transwell chambers for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). Cell migration is expressed as a percentage of that observed with control IgG in normoxia (mean ± SEM; n = 6). (B) MDA-MB-468 cells expressing empty vector (pSUPER), sh-uPAR6 cells, and sh-uPAR12 cells were cultured for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). uPAR mRNA was determined by qPCR. Results are normalized against HPRT-1 and compared with the level observed in the pSUPER cells in normoxia (mean ± SEM; n = 3). (C) pSUPER, sh-uPAR6, and sh-uPAR12 cells were allowed to migrate in Transwell chamber (cell migration) or to invade Matrigel (cell invasion) for 24 h in 21% O 2 (gray bars) or 1.0% O 2 (black bars). Results are expressed as a percentage of that observed with pSUPER cells in normoxia (mean ± SEM; n = 7 and n = 6, respectively). (D) pSUPER, sh-uPAR6, and sh-uPAR12 cells were cultured for 15 h in 21% O 2 (N) or in 1.0% O 2 (H). Cell extracts were affinity precipitated with PAK-1 PBD and subjected to immunoblot analysis to determine GTP-bound Rac1. The original extracts were subjected to immunoblot analysis for tubulin, as a loading control.

    Article Snippet: The Alexa Fluor 594 protein-labeling kit was obtained from Invitrogen. qPCR reagents, including primers and probes for human uPAR, Snail, VEGF, and HPRT-1 were obtained from Applied Biosystems.

    Techniques: Migration, Activation Assay, Multiple Displacement Amplification, Expressing, Plasmid Preparation, Cell Culture, Real-time Polymerase Chain Reaction

    Effect of REAC TO-RGN on p53 gene expression. hADSCs at passages 5, 10, 15, 20, 25, and 30, reaching 80 % confluence, were exposed for 4, 8, or 12 h in the absence or presence of REAC. The amount of p53 mRNA from control ( white bars ) and TO-RGN-treated ( purple bars ) cells was normalized to HPRT1 and was plotted as fold change relative to the mRNA expression at passage 5 (time 0), defined as 1. ( Asterisk ) significantly different from the untreated cells (mean ± S.E.; n = 6) ( P

    Journal: Age

    Article Title: Anti-senescence efficacy of radio-electric asymmetric conveyer technology

    doi: 10.1007/s11357-013-9537-8

    Figure Lengend Snippet: Effect of REAC TO-RGN on p53 gene expression. hADSCs at passages 5, 10, 15, 20, 25, and 30, reaching 80 % confluence, were exposed for 4, 8, or 12 h in the absence or presence of REAC. The amount of p53 mRNA from control ( white bars ) and TO-RGN-treated ( purple bars ) cells was normalized to HPRT1 and was plotted as fold change relative to the mRNA expression at passage 5 (time 0), defined as 1. ( Asterisk ) significantly different from the untreated cells (mean ± S.E.; n = 6) ( P

    Article Snippet: Specific primers for p16, p19, p21, p53, and HPRT1 used in this study were from Invitrogen and previously described (Maioli et al. ; Bea et al. ; Isenmann et al. ; Vandesompele et al. ; Maioli et al. ).

    Techniques: Expressing

    Effect of REAC TO-RGN on p21 gene expression. hADSCs at passages 5, 10, 15, 20, 25, and 30, reaching 80 % confluence, were exposed for 4, 8, or 12 h in the absence or presence of REAC. The amount of p21 mRNA from control ( white bars ) and TO-RGN-treated ( green bars ) cells was normalized to HPRT1 and was plotted as fold change relative to the mRNA expression at passage 5 (time 0), defined as 1. ( Asterisk ) significantly different from the untreated cells (mean ± S.E.; n = 6) ( P

    Journal: Age

    Article Title: Anti-senescence efficacy of radio-electric asymmetric conveyer technology

    doi: 10.1007/s11357-013-9537-8

    Figure Lengend Snippet: Effect of REAC TO-RGN on p21 gene expression. hADSCs at passages 5, 10, 15, 20, 25, and 30, reaching 80 % confluence, were exposed for 4, 8, or 12 h in the absence or presence of REAC. The amount of p21 mRNA from control ( white bars ) and TO-RGN-treated ( green bars ) cells was normalized to HPRT1 and was plotted as fold change relative to the mRNA expression at passage 5 (time 0), defined as 1. ( Asterisk ) significantly different from the untreated cells (mean ± S.E.; n = 6) ( P

    Article Snippet: Specific primers for p16, p19, p21, p53, and HPRT1 used in this study were from Invitrogen and previously described (Maioli et al. ; Bea et al. ; Isenmann et al. ; Vandesompele et al. ; Maioli et al. ).

    Techniques: Expressing

    Effect of REAC TO-RGN on ARF gene expression. hADSCs at passages 5, 10, 15, 20, 25, and 30, reaching 80 % confluence, were exposed for 4, 8, or 12 h in the absence or presence of REAC. The amount of ARF mRNA from control ( white bars ) and TO-RGN-treated ( red bars ) cells was normalized to HPRT1 and was plotted as fold change relative to the mRNA expression at passage 5 (time 0), defined as 1. ( Asterisk ) significantly different from the untreated cells (mean ± S.E.; n = 6) ( P

    Journal: Age

    Article Title: Anti-senescence efficacy of radio-electric asymmetric conveyer technology

    doi: 10.1007/s11357-013-9537-8

    Figure Lengend Snippet: Effect of REAC TO-RGN on ARF gene expression. hADSCs at passages 5, 10, 15, 20, 25, and 30, reaching 80 % confluence, were exposed for 4, 8, or 12 h in the absence or presence of REAC. The amount of ARF mRNA from control ( white bars ) and TO-RGN-treated ( red bars ) cells was normalized to HPRT1 and was plotted as fold change relative to the mRNA expression at passage 5 (time 0), defined as 1. ( Asterisk ) significantly different from the untreated cells (mean ± S.E.; n = 6) ( P

    Article Snippet: Specific primers for p16, p19, p21, p53, and HPRT1 used in this study were from Invitrogen and previously described (Maioli et al. ; Bea et al. ; Isenmann et al. ; Vandesompele et al. ; Maioli et al. ).

    Techniques: Expressing

    Effect of REAC TO-RGN on p16INK4a gene expression. hADSCs at passages 5, 10, 15, 20, 25, and 30, reaching 80 % confluence, were exposed for 4, 8, or 12 h in the absence or presence of REAC. The amount of p16INK4a mRNA from control ( white bars ) and TO-RGN treated ( blue bars ) cells was normalized to HPRT1 and was plotted as fold change relative to the mRNA expression at passage 5 (time 0), defined as 1. ( Asterisk ) significantly different from the untreated cells (mean ± S.E.; n = 6) ( P

    Journal: Age

    Article Title: Anti-senescence efficacy of radio-electric asymmetric conveyer technology

    doi: 10.1007/s11357-013-9537-8

    Figure Lengend Snippet: Effect of REAC TO-RGN on p16INK4a gene expression. hADSCs at passages 5, 10, 15, 20, 25, and 30, reaching 80 % confluence, were exposed for 4, 8, or 12 h in the absence or presence of REAC. The amount of p16INK4a mRNA from control ( white bars ) and TO-RGN treated ( blue bars ) cells was normalized to HPRT1 and was plotted as fold change relative to the mRNA expression at passage 5 (time 0), defined as 1. ( Asterisk ) significantly different from the untreated cells (mean ± S.E.; n = 6) ( P

    Article Snippet: Specific primers for p16, p19, p21, p53, and HPRT1 used in this study were from Invitrogen and previously described (Maioli et al. ; Bea et al. ; Isenmann et al. ; Vandesompele et al. ; Maioli et al. ).

    Techniques: Expressing

    IUGR+HFD female rats had decreased serum HDL (A) compared to Con+HFD female rats, increased hepatic triglycerides (B) and cholesterol (C) , decreased hepatic bile acids (C) , decreased Cyp7a1 protein, and increased miR-122 (D) compared to Con+Reg female rats. A maternal HFD increased cholesterol and hepatic Cyp7a1 mRNA and protein in both sexes in IUGR and control rats (D) . Data shown as scatter plots of individual rats with mean ± SD with a minimum n = 6 rats per sex, per intrauterine environment, each from separate litters. Data from male rats is shown on the left of the figure, and from female rats is shown on the right. Groups are denoted as follows: Con+Reg data are shown in black, Con+HFD data are shown in yellow, IUGR+Reg data are shown in blue, and IUGR+HFD data are shown in red, with group names listed below the graphs on the bottom of the figure. Western blot images of Lxrα, Cyp7a1, and Hprt1 protein are shown above the graphical representation of band densitometry. The kilodalton (kDa) marker is shown on the left lane of the western blot image; the 50 kDa marker is shown in the Lxrα blot, the 60 kDa marker is shown in the Cyp7a1 blot, and the 30 kDa marker is shown in the Hprt1 blot and the Hprt1 image was obtained from the blot with Cyp7a1. A p -value ≤0.05 is denoted with an asterisk ( ∗ ) for any group data compared to sex-matched Con+Reg, and a p -value ≤ 0.05 is denoted with a hatched line (†) for IUGR+HFD data compared to sex-matched Con+HFD.

    Journal: Frontiers in Physiology

    Article Title: Prenatal Exposure to a Maternal High Fat Diet Increases Hepatic Cholesterol Accumulation in Intrauterine Growth Restricted Rats in Part Through MicroRNA-122 Inhibition of Cyp7a1

    doi: 10.3389/fphys.2018.00645

    Figure Lengend Snippet: IUGR+HFD female rats had decreased serum HDL (A) compared to Con+HFD female rats, increased hepatic triglycerides (B) and cholesterol (C) , decreased hepatic bile acids (C) , decreased Cyp7a1 protein, and increased miR-122 (D) compared to Con+Reg female rats. A maternal HFD increased cholesterol and hepatic Cyp7a1 mRNA and protein in both sexes in IUGR and control rats (D) . Data shown as scatter plots of individual rats with mean ± SD with a minimum n = 6 rats per sex, per intrauterine environment, each from separate litters. Data from male rats is shown on the left of the figure, and from female rats is shown on the right. Groups are denoted as follows: Con+Reg data are shown in black, Con+HFD data are shown in yellow, IUGR+Reg data are shown in blue, and IUGR+HFD data are shown in red, with group names listed below the graphs on the bottom of the figure. Western blot images of Lxrα, Cyp7a1, and Hprt1 protein are shown above the graphical representation of band densitometry. The kilodalton (kDa) marker is shown on the left lane of the western blot image; the 50 kDa marker is shown in the Lxrα blot, the 60 kDa marker is shown in the Cyp7a1 blot, and the 30 kDa marker is shown in the Hprt1 blot and the Hprt1 image was obtained from the blot with Cyp7a1. A p -value ≤0.05 is denoted with an asterisk ( ∗ ) for any group data compared to sex-matched Con+Reg, and a p -value ≤ 0.05 is denoted with a hatched line (†) for IUGR+HFD data compared to sex-matched Con+HFD.

    Article Snippet: Hepatic mRNA Quantification Semi-quantitative real-time reverse-transcriptase PCR was performed on hepatic cDNA using Hprt1 message (Proteintech) as an internal control, since C t values of hepatic Hprt1 did not differ between intrauterine conditions.

    Techniques: Western Blot, Marker

    Injection of a miR-122 inhibitor into IUGR+HFD female rats decreased hepatic miR-122 (A) , increased Cyp7a1 protein (B) , and decreased hepatic cholesterol (C) . Injections did not change serum total or HDL cholesterol (D) . Data shown as scatter plots of individual rats with mean ± SD with a minimum n = 6 rats per sex, per intrauterine environment, per type of injection, each from separate litters. Groups are denoted as follows: CH+Scr data are shown in black, CH+Mim data are shown in yellow, IH+Scr data are shown in blue, and IH+Inh data are shown in red. Western blot images of Cyp7a1 and Hprt1 protein are shown above the graphical representation of band densitometry. The kilodalton (kDa) marker is shown on the left lane of the western blot image; the 60 kDa marker is shown in the Cyp7a1 blot, and the 30 kDa marker is shown in the Hprt1 blot. A p -value ≤ 0.05 is denoted with an asterisk ( ∗ ) for any group data that differed as noted by the line above the graph.

    Journal: Frontiers in Physiology

    Article Title: Prenatal Exposure to a Maternal High Fat Diet Increases Hepatic Cholesterol Accumulation in Intrauterine Growth Restricted Rats in Part Through MicroRNA-122 Inhibition of Cyp7a1

    doi: 10.3389/fphys.2018.00645

    Figure Lengend Snippet: Injection of a miR-122 inhibitor into IUGR+HFD female rats decreased hepatic miR-122 (A) , increased Cyp7a1 protein (B) , and decreased hepatic cholesterol (C) . Injections did not change serum total or HDL cholesterol (D) . Data shown as scatter plots of individual rats with mean ± SD with a minimum n = 6 rats per sex, per intrauterine environment, per type of injection, each from separate litters. Groups are denoted as follows: CH+Scr data are shown in black, CH+Mim data are shown in yellow, IH+Scr data are shown in blue, and IH+Inh data are shown in red. Western blot images of Cyp7a1 and Hprt1 protein are shown above the graphical representation of band densitometry. The kilodalton (kDa) marker is shown on the left lane of the western blot image; the 60 kDa marker is shown in the Cyp7a1 blot, and the 30 kDa marker is shown in the Hprt1 blot. A p -value ≤ 0.05 is denoted with an asterisk ( ∗ ) for any group data that differed as noted by the line above the graph.

    Article Snippet: Hepatic mRNA Quantification Semi-quantitative real-time reverse-transcriptase PCR was performed on hepatic cDNA using Hprt1 message (Proteintech) as an internal control, since C t values of hepatic Hprt1 did not differ between intrauterine conditions.

    Techniques: Injection, Western Blot, Marker

    Expression of COPZ2 in thyroid tumor cell lines Real-time PCR analysis of COPZ2 gene expression; results are presented as log10-transformed relative quantity (RQ) of COPZ2 mRNA normalized for HPRT1 housekeeping gene expression. Data represent the mean +/− sd of three independent experiments.

    Journal: Oncotarget

    Article Title: Identification of thyroid tumor cell vulnerabilities through a siRNA-based functional screening

    doi:

    Figure Lengend Snippet: Expression of COPZ2 in thyroid tumor cell lines Real-time PCR analysis of COPZ2 gene expression; results are presented as log10-transformed relative quantity (RQ) of COPZ2 mRNA normalized for HPRT1 housekeeping gene expression. Data represent the mean +/− sd of three independent experiments.

    Article Snippet: Hs00212698_m1 was used for COPZ2 expression; human HPRT1 (HPRT-Hs99999909_A1) was used as housekeeping gene for the normalization among samples.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Transformation Assay

    Effect of rs1790834 genotypes on expression of CYB5A and steroidogenic genes. Real-time quantitative PCR analysis of mRNA expression in 22 RA synovial fibroblast lines (rs1790834 genotype GG, n = 15; AA/AG genotypes, n = 7). (A) Synovial fibroblasts express all genes, which are necessary for synthesis of biologically active androgens. Mean ± SD of expression levels normalized to HPRT1 of combined data for all genotypes. (B) rs1790834 genotype-dependent expression levels, which are normalized to the median value of the combined expression data for each gene. In the box-and-whisker plots the genotypes with the rare allele A were grouped together (AA, n = 1; AG, n = 6). The boxes represent the 25th to 75th percentiles, and horizontal lines within the box represent median values. The whiskers show the 10th and 90th percentiles, respectively, and dots represent individual values exceeding these limits. ***Significant rs1790834 genotype-dependent expression, P

    Journal: Arthritis Research & Therapy

    Article Title: CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women

    doi: 10.1186/s13075-015-0574-9

    Figure Lengend Snippet: Effect of rs1790834 genotypes on expression of CYB5A and steroidogenic genes. Real-time quantitative PCR analysis of mRNA expression in 22 RA synovial fibroblast lines (rs1790834 genotype GG, n = 15; AA/AG genotypes, n = 7). (A) Synovial fibroblasts express all genes, which are necessary for synthesis of biologically active androgens. Mean ± SD of expression levels normalized to HPRT1 of combined data for all genotypes. (B) rs1790834 genotype-dependent expression levels, which are normalized to the median value of the combined expression data for each gene. In the box-and-whisker plots the genotypes with the rare allele A were grouped together (AA, n = 1; AG, n = 6). The boxes represent the 25th to 75th percentiles, and horizontal lines within the box represent median values. The whiskers show the 10th and 90th percentiles, respectively, and dots represent individual values exceeding these limits. ***Significant rs1790834 genotype-dependent expression, P

    Article Snippet: Relative gene expression was normalized to HPRT1 mRNA (Hs01003267_m1) levels using the comparative cycle threshold (Ct) method and presented as expression ratio using 2-ΔΔCt [ ].

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Whisker Assay

    Sex-dependent expression of Oat1 and Oat3 in rat cortical kidney slices. Levels of β-actin, Hprt1, Oat1 and Oat3 were analyzed by TaqMan® real-time PCR in total RNA isolated from four male and four female cortical kidney slices. The mRNA expression of reference genes β-actin and Hprt1 were investigated by comparing their Ct values between male and female (2A). Levels of Oat1 and Oat3 were determined using 2 −ΔΔCt method, at which β-actin was the reference gene (2B). ΔΔCt values were calculated as ΔCt male - ΔCt female. n male = 4; n female = 4.

    Journal: PLoS ONE

    Article Title: Male-Dominant Activation of Rat Renal Organic Anion Transporter 1 (Oat1) and 3 (Oat3) Expression by Transcription Factor BCL6

    doi: 10.1371/journal.pone.0035556

    Figure Lengend Snippet: Sex-dependent expression of Oat1 and Oat3 in rat cortical kidney slices. Levels of β-actin, Hprt1, Oat1 and Oat3 were analyzed by TaqMan® real-time PCR in total RNA isolated from four male and four female cortical kidney slices. The mRNA expression of reference genes β-actin and Hprt1 were investigated by comparing their Ct values between male and female (2A). Levels of Oat1 and Oat3 were determined using 2 −ΔΔCt method, at which β-actin was the reference gene (2B). ΔΔCt values were calculated as ΔCt male - ΔCt female. n male = 4; n female = 4.

    Article Snippet: Following TaqMan® Gene Expression Assays were used: β-actin, Rn00667869_m1; Hprt1, Rn01527840_m1; hepatocyte nuclear factor 1α (Hnf1α), Rn00562020_m1; hepatocyte nuclear factor 1β (Hnf1β), Rn00447453_m1; hepatocyte nuclear factor 4α (Hnf4α), Rn00573309_m1; androgen receptor (rAR), Rn00560747_m1; Oat1, Rn00568143_m1; Oat3, Rn00580082_m1; polymerase (RNA) III (DNA directed) polypeptide G (Polr3g), Rn01494192_m1; hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1), Rn00563388_g1; B-cell CLL/lymphoma 6 (BCL6), Rn01404339_m1.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Isolation

    PDGF-PDGFRβ signaling regulates key downstream signaling mediators. qPCR analyses on mRNA levels of (A) MDM2 and (B) MDM4 relative to untreated Y79 cells under the cell culture conditions described above. HPRT1 used as housekeeping gene. Western blot analyses (C) of MDM2 activity and (D) AKT activity after disruption of the PDGFRβ signaling cascade. IM, *P = 0.0275; rhPDGF + IM, **P = 0.0011; AKT, *P = 0.0054. Evaluation of the antiapoptotic mediator (E) BCL-2 by Western blot (**P = 0.001 and ***P = 0.0002); and the proapoptotic cleaved (active) capsase-3 by ELISA (F), *P = 0.007. (G–L) Downregulation of the VEGFR signaling when PDGFRβ is inhibited. (G) VEGFA mRNA levels were measured by qPCR analysis in both Y79 cells and (H) PDX samples. (I) Both VEGFA and (J) VEGFR2 mRNA levels were measured across respective treatments as well as (K) Western blot analyses of VEGFR2 activity; *P = 0.0191. (L) Measurement of VEGFA levels in a cohort of vitreous samples from healthy controls compared to vitreous of Rb patients. ****P = 0.0001. (M–O) Assessment of the NF-κB signaling. Representing images of Y79 cells (M) labeled and analyzed for nuclear (labeled with DRAQ5) translocation of the p65 subunit of NF-κB (AlexaFluor 488 conjugated), ***P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Targeting the Platelet-Derived Growth Factor-beta Stimulatory Circuitry to Control Retinoblastoma Seeds

    doi: 10.1167/iovs.18-24359

    Figure Lengend Snippet: PDGF-PDGFRβ signaling regulates key downstream signaling mediators. qPCR analyses on mRNA levels of (A) MDM2 and (B) MDM4 relative to untreated Y79 cells under the cell culture conditions described above. HPRT1 used as housekeeping gene. Western blot analyses (C) of MDM2 activity and (D) AKT activity after disruption of the PDGFRβ signaling cascade. IM, *P = 0.0275; rhPDGF + IM, **P = 0.0011; AKT, *P = 0.0054. Evaluation of the antiapoptotic mediator (E) BCL-2 by Western blot (**P = 0.001 and ***P = 0.0002); and the proapoptotic cleaved (active) capsase-3 by ELISA (F), *P = 0.007. (G–L) Downregulation of the VEGFR signaling when PDGFRβ is inhibited. (G) VEGFA mRNA levels were measured by qPCR analysis in both Y79 cells and (H) PDX samples. (I) Both VEGFA and (J) VEGFR2 mRNA levels were measured across respective treatments as well as (K) Western blot analyses of VEGFR2 activity; *P = 0.0191. (L) Measurement of VEGFA levels in a cohort of vitreous samples from healthy controls compared to vitreous of Rb patients. ****P = 0.0001. (M–O) Assessment of the NF-κB signaling. Representing images of Y79 cells (M) labeled and analyzed for nuclear (labeled with DRAQ5) translocation of the p65 subunit of NF-κB (AlexaFluor 488 conjugated), ***P

    Article Snippet: Gene expression assays (Thermo Scientific) included the following genes: PDGFRA (Hs00998018_m1), PDGFRB (Hs01019589_m1), PDGFA (Hs00234994_m1), PDGFB (Hs_00966522_m1), MDM2 (Hs00540450_m1), MDM4 (Hs00910358_m1), VEGFA (Hs00900055_m1), FLT1 (aka VEGFR2, Hs01052961_m1), and HPRT1 (Hs02800695_m1).

    Techniques: Real-time Polymerase Chain Reaction, Cell Culture, Western Blot, Activity Assay, Enzyme-linked Immunosorbent Assay, Labeling, Translocation Assay

    Expression of the active and nonactive forms of PDGFRβ in Rb. (A–D) Representative images of immunohistochemic staining for expression of nonphosphorylated PDGFRβ and p-PDGFRβ (active) from a cohort of 15 enucleated eyes of Rb patients. Images taken at 20×. (E, F) Representative image of immunohistochemical results of PDGFRβ in vitreous seeds. Images taken at 40×. (G, H) Images from PDX. Images taken at 20×. (I) qPCR analysis of mRNA from PDX for key members of PDGF signaling pathway. Results are represented as mean ± SEM fold change of the target gene over HPRT1, the housekeeping gene. (J) mRNA expression of both PDGFRA and PDGFRB in both Rb cell lines Y79 and Weri-Rb-1. Results are represented as mean ± SEM fold change of the target gene over HPRT1, the housekeeping gene. (K, L) Levels of PDGF-AB (K) and PDGF-BB (L) in vitreous samples from healthy controls vitreous compared to those of Rb patients by ELISA analyses. n = 9 in PDX mRNA analyses with 4 replicates per sample; n = 4 for Y79 and Weri-1 mRNA analyses with 4 replicates per sample; n = 6 in ELISA analyses done in triplicates. All results represent mean ± SEM; **P = 0.0010; ***P = 0.00051.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Targeting the Platelet-Derived Growth Factor-beta Stimulatory Circuitry to Control Retinoblastoma Seeds

    doi: 10.1167/iovs.18-24359

    Figure Lengend Snippet: Expression of the active and nonactive forms of PDGFRβ in Rb. (A–D) Representative images of immunohistochemic staining for expression of nonphosphorylated PDGFRβ and p-PDGFRβ (active) from a cohort of 15 enucleated eyes of Rb patients. Images taken at 20×. (E, F) Representative image of immunohistochemical results of PDGFRβ in vitreous seeds. Images taken at 40×. (G, H) Images from PDX. Images taken at 20×. (I) qPCR analysis of mRNA from PDX for key members of PDGF signaling pathway. Results are represented as mean ± SEM fold change of the target gene over HPRT1, the housekeeping gene. (J) mRNA expression of both PDGFRA and PDGFRB in both Rb cell lines Y79 and Weri-Rb-1. Results are represented as mean ± SEM fold change of the target gene over HPRT1, the housekeeping gene. (K, L) Levels of PDGF-AB (K) and PDGF-BB (L) in vitreous samples from healthy controls vitreous compared to those of Rb patients by ELISA analyses. n = 9 in PDX mRNA analyses with 4 replicates per sample; n = 4 for Y79 and Weri-1 mRNA analyses with 4 replicates per sample; n = 6 in ELISA analyses done in triplicates. All results represent mean ± SEM; **P = 0.0010; ***P = 0.00051.

    Article Snippet: Gene expression assays (Thermo Scientific) included the following genes: PDGFRA (Hs00998018_m1), PDGFRB (Hs01019589_m1), PDGFA (Hs00234994_m1), PDGFB (Hs_00966522_m1), MDM2 (Hs00540450_m1), MDM4 (Hs00910358_m1), VEGFA (Hs00900055_m1), FLT1 (aka VEGFR2, Hs01052961_m1), and HPRT1 (Hs02800695_m1).

    Techniques: Expressing, Staining, Immunohistochemistry, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Individuals with the p.Ala489Thr interferon-induced helicase C domain-containing protein 1 (IFIH1) mutant demonstrate elevated levels of interferon-stimulated genes compared with controls. An interferon score was calculated from the median fold change in RQ value for a panel of six interferon signature genes measured using quantitative polymerase chain reaction – IFI27 (Hs01086370_m1), IFI44L (Hs00199115_m1), IFIT1 (Hs00356631_g1), ISG15 (Hs00192713_m1), RSAD2 (Hs01057264_m1) and SIGLEC1 (Hs00988063_m1) – normalized to the expression levels of HPRT1 with IFIH1-related disease, and is significantly higher than in control samples (**** P

    Journal: The British journal of dermatology

    Article Title: Unusual cutaneous features associated with a heterozygous gain-of-function mutation in IFIH1: overlap between Aicardi–Goutières and Singleton–Merten syndromes

    doi: 10.1111/bjd.14073

    Figure Lengend Snippet: Individuals with the p.Ala489Thr interferon-induced helicase C domain-containing protein 1 (IFIH1) mutant demonstrate elevated levels of interferon-stimulated genes compared with controls. An interferon score was calculated from the median fold change in RQ value for a panel of six interferon signature genes measured using quantitative polymerase chain reaction – IFI27 (Hs01086370_m1), IFI44L (Hs00199115_m1), IFIT1 (Hs00356631_g1), ISG15 (Hs00192713_m1), RSAD2 (Hs01057264_m1) and SIGLEC1 (Hs00988063_m1) – normalized to the expression levels of HPRT1 with IFIH1-related disease, and is significantly higher than in control samples (**** P

    Article Snippet: Assessment of additional proinflammatory cytokines by quantitative polymerase chain reaction ( IL6 , TNFA , NFKB1 , IL1B and IL10 ) showed equivalent expression to that observed in controls (data not shown). (Hs03929096_g1) and 18S RNA (Hs999999001_s1).

    Techniques: Mutagenesis, Real-time Polymerase Chain Reaction, Expressing

    HMGA1 real-time PCR analyses. Relative HMGA1/HPRT1 and HMGA1/GUSB expression in native CT1258, CT1258-EGFP and CT1258-HMGA2-EGFP cells. Error bars are standard deviations. *p≤0.05 indicates a statistical significant increased expression of HMGA1 in CT1258-HMGA2-EGFP cells when compared to native CT1258 and CT1258-EGFP.

    Journal: PLoS ONE

    Article Title: Generation and Characterisation of a Canine EGFP-HMGA2 Prostate Cancer In Vitro Model

    doi: 10.1371/journal.pone.0098788

    Figure Lengend Snippet: HMGA1 real-time PCR analyses. Relative HMGA1/HPRT1 and HMGA1/GUSB expression in native CT1258, CT1258-EGFP and CT1258-HMGA2-EGFP cells. Error bars are standard deviations. *p≤0.05 indicates a statistical significant increased expression of HMGA1 in CT1258-HMGA2-EGFP cells when compared to native CT1258 and CT1258-EGFP.

    Article Snippet: Commercially available TaqMan gene expression assays were used for the analysis of the canine targets SNAI1 (Cf02705362_s1), SNAI2 (Cf02701218_u1) and CDH1 (Cf02697525_m1) as well as for the endogenous controls, canine GUSB (Cf02622808_m1) and canine HPRT1 (Cf02626258_m1) (Applied Biosystems, Darmstadt, Germany).

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    HMGA2 real-time PCR analyses. Relative HMGA2/HPRT1 and HMGA2/GUSB expression in native CT1258, CT1258-EGFP and CT1258-HMGA2-EGFP cells. Error bars are standard deviations. *p≤0.05 indicates a statistical significant expression deregulation of HMGA2 in CT1258-HMGA2-EGFP cells when compared to native CT1258.

    Journal: PLoS ONE

    Article Title: Generation and Characterisation of a Canine EGFP-HMGA2 Prostate Cancer In Vitro Model

    doi: 10.1371/journal.pone.0098788

    Figure Lengend Snippet: HMGA2 real-time PCR analyses. Relative HMGA2/HPRT1 and HMGA2/GUSB expression in native CT1258, CT1258-EGFP and CT1258-HMGA2-EGFP cells. Error bars are standard deviations. *p≤0.05 indicates a statistical significant expression deregulation of HMGA2 in CT1258-HMGA2-EGFP cells when compared to native CT1258.

    Article Snippet: Commercially available TaqMan gene expression assays were used for the analysis of the canine targets SNAI1 (Cf02705362_s1), SNAI2 (Cf02701218_u1) and CDH1 (Cf02697525_m1) as well as for the endogenous controls, canine GUSB (Cf02622808_m1) and canine HPRT1 (Cf02626258_m1) (Applied Biosystems, Darmstadt, Germany).

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    Hepatic protein levels of Ldlr relative to control protein Hprt1. * P ≤ 0.05 compared to sex‐matched Con+Reg, † P ≤ 0.05 compared to sex‐matched Con+ HFD . Data from Con+Reg rats are shown in white bars, Con+ HFD rats are shown in black bars, IUGR +Reg rats shown in light gray bars, and IUGR + HFD rats are shown in dark gray bars. Western blot image is depicted below the graph, with a representative Hprt1 control blot at the bottom. N = 6 per sex, diet, and surgical intervention. Data are shown as mean ± SEM . HFD, high‐saturated‐fat diet; IUGR, intrauterine growth restriction.

    Journal: Physiological Reports

    Article Title: Intrauterine growth restriction combined with a maternal high‐fat diet increases hepatic cholesterol and low‐density lipoprotein receptor activity in rats. Intrauterine growth restriction combined with a maternal high‐fat diet increases hepatic cholesterol and low‐density lipoprotein receptor activity in rats

    doi: 10.14814/phy2.12862

    Figure Lengend Snippet: Hepatic protein levels of Ldlr relative to control protein Hprt1. * P ≤ 0.05 compared to sex‐matched Con+Reg, † P ≤ 0.05 compared to sex‐matched Con+ HFD . Data from Con+Reg rats are shown in white bars, Con+ HFD rats are shown in black bars, IUGR +Reg rats shown in light gray bars, and IUGR + HFD rats are shown in dark gray bars. Western blot image is depicted below the graph, with a representative Hprt1 control blot at the bottom. N = 6 per sex, diet, and surgical intervention. Data are shown as mean ± SEM . HFD, high‐saturated‐fat diet; IUGR, intrauterine growth restriction.

    Article Snippet: Hprt1 (15059‐1‐AP, Proteintech, Chicago, IL) was used as a loading control, as Hprt1 did not differ by intrauterine conditions or diet.

    Techniques: Western Blot

    Protein levels of proteins involved in HDL uptake from the blood to the liver relative to control protein Hprt1. * P ≤ 0.05 compared to sex‐matched Con+Reg, † P ≤ 0.05 compared to sex‐matched Con+ HFD . Data from Con+Reg rats are shown in white bars, Con+ HFD rats are shown in black bars, IUGR +Reg rats shown in light gray bars, and IUGR + HFD rats are shown in dark gray bars. Western blot image is depicted below the graph, with a representative Hprt1 control blot at the bottom. N = 6 per sex, diet, and surgical intervention. Data are shown as mean ± SEM . HDL, high‐density lipoprotein; HFD, high‐saturated‐fat diet; IUGR, intrauterine growth restriction.

    Journal: Physiological Reports

    Article Title: Intrauterine growth restriction combined with a maternal high‐fat diet increases hepatic cholesterol and low‐density lipoprotein receptor activity in rats. Intrauterine growth restriction combined with a maternal high‐fat diet increases hepatic cholesterol and low‐density lipoprotein receptor activity in rats

    doi: 10.14814/phy2.12862

    Figure Lengend Snippet: Protein levels of proteins involved in HDL uptake from the blood to the liver relative to control protein Hprt1. * P ≤ 0.05 compared to sex‐matched Con+Reg, † P ≤ 0.05 compared to sex‐matched Con+ HFD . Data from Con+Reg rats are shown in white bars, Con+ HFD rats are shown in black bars, IUGR +Reg rats shown in light gray bars, and IUGR + HFD rats are shown in dark gray bars. Western blot image is depicted below the graph, with a representative Hprt1 control blot at the bottom. N = 6 per sex, diet, and surgical intervention. Data are shown as mean ± SEM . HDL, high‐density lipoprotein; HFD, high‐saturated‐fat diet; IUGR, intrauterine growth restriction.

    Article Snippet: Hprt1 (15059‐1‐AP, Proteintech, Chicago, IL) was used as a loading control, as Hprt1 did not differ by intrauterine conditions or diet.

    Techniques: Western Blot

    Protein levels of proteins involved in hepatic de novo cholesterol synthesis relative to control protein Hprt1. * P ≤ 0.05 compared to sex‐matched Con+Reg, † P ≤ 0.05 compared to sex‐matched Con+ HFD . Data from Con+Reg rats are shown in white bars, Con+ HFD rats are shown in black bars, IUGR +Reg rats shown in light gray bars, and IUGR + HFD rats are shown in dark gray bars. Western blot image is depicted below the graph, with a representative Hprt1 control blot at the bottom. N = 6 per sex, diet, and surgical intervention. Data are shown as mean ± SEM . HFD, high‐saturated‐fat diet; IUGR, Intrauterine growth restriction.

    Journal: Physiological Reports

    Article Title: Intrauterine growth restriction combined with a maternal high‐fat diet increases hepatic cholesterol and low‐density lipoprotein receptor activity in rats. Intrauterine growth restriction combined with a maternal high‐fat diet increases hepatic cholesterol and low‐density lipoprotein receptor activity in rats

    doi: 10.14814/phy2.12862

    Figure Lengend Snippet: Protein levels of proteins involved in hepatic de novo cholesterol synthesis relative to control protein Hprt1. * P ≤ 0.05 compared to sex‐matched Con+Reg, † P ≤ 0.05 compared to sex‐matched Con+ HFD . Data from Con+Reg rats are shown in white bars, Con+ HFD rats are shown in black bars, IUGR +Reg rats shown in light gray bars, and IUGR + HFD rats are shown in dark gray bars. Western blot image is depicted below the graph, with a representative Hprt1 control blot at the bottom. N = 6 per sex, diet, and surgical intervention. Data are shown as mean ± SEM . HFD, high‐saturated‐fat diet; IUGR, Intrauterine growth restriction.

    Article Snippet: Hprt1 (15059‐1‐AP, Proteintech, Chicago, IL) was used as a loading control, as Hprt1 did not differ by intrauterine conditions or diet.

    Techniques: Western Blot

    Protein levels of proteins involved in hepatic cholesterol catabolism to bile acids relative to control protein Hprt1. * P ≤ 0.05 compared to sex‐matched Con+Reg, † P ≤ 0.05 compared to sex‐matched Con+ HFD . Data from Con+Reg rats are shown in white bars, Con+ HFD rats are shown in black bars, IUGR +Reg rats shown in light gray bars, and IUGR + HFD rats are shown in dark gray bars. Western blot imag e is depicted below the graph, with a representative Hprt1 control blot at the bottom. N = 6 per sex, diet, and surgical intervention. Data are shown as mean ± SEM . HFD, high‐saturated‐fat diet; IUGR, intrauterine growth restriction.

    Journal: Physiological Reports

    Article Title: Intrauterine growth restriction combined with a maternal high‐fat diet increases hepatic cholesterol and low‐density lipoprotein receptor activity in rats. Intrauterine growth restriction combined with a maternal high‐fat diet increases hepatic cholesterol and low‐density lipoprotein receptor activity in rats

    doi: 10.14814/phy2.12862

    Figure Lengend Snippet: Protein levels of proteins involved in hepatic cholesterol catabolism to bile acids relative to control protein Hprt1. * P ≤ 0.05 compared to sex‐matched Con+Reg, † P ≤ 0.05 compared to sex‐matched Con+ HFD . Data from Con+Reg rats are shown in white bars, Con+ HFD rats are shown in black bars, IUGR +Reg rats shown in light gray bars, and IUGR + HFD rats are shown in dark gray bars. Western blot imag e is depicted below the graph, with a representative Hprt1 control blot at the bottom. N = 6 per sex, diet, and surgical intervention. Data are shown as mean ± SEM . HFD, high‐saturated‐fat diet; IUGR, intrauterine growth restriction.

    Article Snippet: Hprt1 (15059‐1‐AP, Proteintech, Chicago, IL) was used as a loading control, as Hprt1 did not differ by intrauterine conditions or diet.

    Techniques: Western Blot

    Protein levels of proteins involved in hepatic VLDL export to the blood relative to control protein Hprt1. * P ≤ 0.05 compared to sex‐matched Con+Reg, † P ≤ 0.05 compared to sex‐matched Con+ HFD . Data from Con+Reg rats are shown in white bars, Con+ HFD rats are shown in black bars, IUGR +Reg rats shown in light gray bars, and IUGR + HFD rats are shown in dark gray bars. Western blot image is depicted below the graph, with a representative Hprt1 control blot at the bottom. N = 6 per sex, diet, and surgical intervention. Data are shown as mean ± SEM . HFD, high‐saturated‐fat diet; IUGR, intrauterine growth restriction; LDL, low‐density lipoprotein; VLDL, very low‐density lipoprotein.

    Journal: Physiological Reports

    Article Title: Intrauterine growth restriction combined with a maternal high‐fat diet increases hepatic cholesterol and low‐density lipoprotein receptor activity in rats. Intrauterine growth restriction combined with a maternal high‐fat diet increases hepatic cholesterol and low‐density lipoprotein receptor activity in rats

    doi: 10.14814/phy2.12862

    Figure Lengend Snippet: Protein levels of proteins involved in hepatic VLDL export to the blood relative to control protein Hprt1. * P ≤ 0.05 compared to sex‐matched Con+Reg, † P ≤ 0.05 compared to sex‐matched Con+ HFD . Data from Con+Reg rats are shown in white bars, Con+ HFD rats are shown in black bars, IUGR +Reg rats shown in light gray bars, and IUGR + HFD rats are shown in dark gray bars. Western blot image is depicted below the graph, with a representative Hprt1 control blot at the bottom. N = 6 per sex, diet, and surgical intervention. Data are shown as mean ± SEM . HFD, high‐saturated‐fat diet; IUGR, intrauterine growth restriction; LDL, low‐density lipoprotein; VLDL, very low‐density lipoprotein.

    Article Snippet: Hprt1 (15059‐1‐AP, Proteintech, Chicago, IL) was used as a loading control, as Hprt1 did not differ by intrauterine conditions or diet.

    Techniques: Western Blot