Journal: Scientific Reports

Article Title: Comprehensive circRNA expression profile during ischemic postconditioning attenuating hepatic ischemia/reperfusion injury

doi: 10.1038/s41598-018-36443-8

Figure Lengend Snippet: Validation of selected circRNAs, miRNAs and mRNAs by qRT-PCR. ( a ) 6 circRNAs were significantly amplified by qRT-PCR and consistent with the microarray results. (b) 9 miRNAs were significantly amplified by qRT-PCR and were inversely correlated with their corresponding circRNAs. (c) 6 mRNAs were significantly amplified by qRT-PCR while only Epha2, Arhgap32, Heatr1 and Egr1 had an identical expression trend with their corresponding circRNAs. * p < 0.05, ** p < 0.01.

Article Snippet: To identify predicted circRNA and miRNA interactions, Arraystar’s in-house miRNA target prediction software was applied on the basis of TargetScan and miRanda algorithms.

Techniques: Biomarker Discovery, Quantitative RT-PCR, Amplification, Microarray, Expressing

Journal: Scientific Reports

Article Title: Comprehensive circRNA expression profile during ischemic postconditioning attenuating hepatic ischemia/reperfusion injury

doi: 10.1038/s41598-018-36443-8

Figure Lengend Snippet: The circRNA-miRNA-mRNA network analysis. The network consists of 6 circRNAs, 47 miRNAs and 90 mRNAs.

Article Snippet: To identify predicted circRNA and miRNA interactions, Arraystar’s in-house miRNA target prediction software was applied on the basis of TargetScan and miRanda algorithms.

Techniques:

Journal: Scientific Reports

Article Title: Comprehensive circRNA expression profile during ischemic postconditioning attenuating hepatic ischemia/reperfusion injury

doi: 10.1038/s41598-018-36443-8

Figure Lengend Snippet: mmu_circRNA_005186 acted as a miRNA Sponge for miR-124-3p and regulated the expression of Epha2. ( a ) Predicted binding site of miR-124-3p in mmu_circRNA_005186 and a diagram for construction of mutated reporter plasmid (mmu_circRNA_005186-Mut). (b) Dual-luciferase reporter assay showed that miR-124-3p mimics significantly repressed the luciferase activity of mmu_circRNA_005186-WT but not the mutant one in 293T cells. (c) Predicted binding site of miR-124-3p in Epha2 3′-UTR. And a diagram for construction of mutated reporter plasmid (Epha2 3′-UTR-Mut). (d) Dual-luciferase reporter assay showed that miR-124-3p mimics significantly repressed the luciferase activity of Epha2 3′-UTR-Mut but not the mutant one in 293T cells. (e) After transfection with si-005186, the expression level of mmu_circRNA_005186 was significantly inhibited in RAW264.7 cells. (f) The expression level of miR-124-3p was significantly upregulated after mmu_circRNA_005186 silencing in RAW264.7 cells. (g) The expression level of Epha2 was significantly inhibited after mmu_circRNA_005186 silencing in RAW264.7 cells. * p < 0.05, ** p < 0.01.

Article Snippet: To identify predicted circRNA and miRNA interactions, Arraystar’s in-house miRNA target prediction software was applied on the basis of TargetScan and miRanda algorithms.

Techniques: Expressing, Binding Assay, Plasmid Preparation, Luciferase, Reporter Assay, Activity Assay, Mutagenesis, Transfection