horseradish peroxidase-conjugated secondary antibodies Search Results


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  • 99
    Bio-Rad horseradish peroxidaseconjugated secondary antibody
    Full-length suPAR increases adhesion of KG1 cells to fibronectin Panel A: KG1 cells were resuspended in 1mg/ml BSA-RPMI at a density of 1×10 6 cells/ml, pre-incubated with or without suPAR (20 nM), uPAR 84-95 or scrambled peptide (10 nM) for 30′ at 37°C and plated on plastic-bound fibronectin for 16 h at 4°C. Attached cells were fixed with 3% PFA and stained with crystal violet; stain was eluted and its absorbance at 540 nm was measured by a spectrophotometer. (*) p≤0.05, as determined by the Student's t test. Panel B: suPAR-treated or untreated KG1 cells were fixed with PFA, incubated with antibodies against total or active β1 integrin or with nonimmune mouse Ig and, then, with FITC <t>anti-mouse</t> IgG. Cells were finally analyzed by flow cytometry using a FACScan (MFI: Mean Fluorescence Intensity). (*) p≤0.05, as determined by the Student's t test. Panel C: Cell surface antigens of suPAR-treated or untreated KG1 cells were biotinylated with EZ-link sulfo-NHS-LC-biotin. Cells were lysed; 50 μg of cell lysates were analyzed by Western blot with an antibody against total β1 integrin, 400 μg of cell lysates were incubated with an antibody against active β1 integrin or nonimmune mouse Ig. Immunocomplexes were then precipitated by protein A-Sepharose, eluted and analyzed by Western blot with <t>horseradish</t> <t>peroxidase-conjugated</t> streptavidin.
    Horseradish Peroxidaseconjugated Secondary Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 314 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega horseradish peroxidase conjugated secondary antibodies
    Full-length suPAR increases adhesion of KG1 cells to fibronectin Panel A: KG1 cells were resuspended in 1mg/ml BSA-RPMI at a density of 1×10 6 cells/ml, pre-incubated with or without suPAR (20 nM), uPAR 84-95 or scrambled peptide (10 nM) for 30′ at 37°C and plated on plastic-bound fibronectin for 16 h at 4°C. Attached cells were fixed with 3% PFA and stained with crystal violet; stain was eluted and its absorbance at 540 nm was measured by a spectrophotometer. (*) p≤0.05, as determined by the Student's t test. Panel B: suPAR-treated or untreated KG1 cells were fixed with PFA, incubated with antibodies against total or active β1 integrin or with nonimmune mouse Ig and, then, with FITC <t>anti-mouse</t> IgG. Cells were finally analyzed by flow cytometry using a FACScan (MFI: Mean Fluorescence Intensity). (*) p≤0.05, as determined by the Student's t test. Panel C: Cell surface antigens of suPAR-treated or untreated KG1 cells were biotinylated with EZ-link sulfo-NHS-LC-biotin. Cells were lysed; 50 μg of cell lysates were analyzed by Western blot with an antibody against total β1 integrin, 400 μg of cell lysates were incubated with an antibody against active β1 integrin or nonimmune mouse Ig. Immunocomplexes were then precipitated by protein A-Sepharose, eluted and analyzed by Western blot with <t>horseradish</t> <t>peroxidase-conjugated</t> streptavidin.
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 400 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare horseradish peroxidase conjugated secondary antibody
    A) Induced SR-BI expression in HEK293 cells. Expression of SR-BI was induced by different concentrations of Doxycycline as indicated on the figure. Western blot analysis was performed with anti-SR-BI <t>antibody</t> (Novus Biologicals, NB400-113). <t>Horseradish</t> <t>peroxidase-conjugated</t> <t>secondary</t> antibody and the enhanced chemiluminescent substrate system (Amersham) were used. B) Bodipy-CE uptake is dependent on the expression level of SR-BI. After Doxycycline induction, HEK293[pTRE-tight-SR-BI] cells were incubated with 10 µg/mL DiO-HDL. The selective uptake was determined as described in Experimental Procedures. Data are presented as mean relative fluorescence units from triplicate or duplicate wells. Error bars represent ±SD.
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 4259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche horseradish peroxidase conjugated secondary antibody
    A) Induced SR-BI expression in HEK293 cells. Expression of SR-BI was induced by different concentrations of Doxycycline as indicated on the figure. Western blot analysis was performed with anti-SR-BI <t>antibody</t> (Novus Biologicals, NB400-113). <t>Horseradish</t> <t>peroxidase-conjugated</t> <t>secondary</t> antibody and the enhanced chemiluminescent substrate system (Amersham) were used. B) Bodipy-CE uptake is dependent on the expression level of SR-BI. After Doxycycline induction, HEK293[pTRE-tight-SR-BI] cells were incubated with 10 µg/mL DiO-HDL. The selective uptake was determined as described in Experimental Procedures. Data are presented as mean relative fluorescence units from triplicate or duplicate wells. Error bars represent ±SD.
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam horseradish peroxidase conjugated secondary antibody
    A) Induced SR-BI expression in HEK293 cells. Expression of SR-BI was induced by different concentrations of Doxycycline as indicated on the figure. Western blot analysis was performed with anti-SR-BI <t>antibody</t> (Novus Biologicals, NB400-113). <t>Horseradish</t> <t>peroxidase-conjugated</t> <t>secondary</t> antibody and the enhanced chemiluminescent substrate system (Amersham) were used. B) Bodipy-CE uptake is dependent on the expression level of SR-BI. After Doxycycline induction, HEK293[pTRE-tight-SR-BI] cells were incubated with 10 µg/mL DiO-HDL. The selective uptake was determined as described in Experimental Procedures. Data are presented as mean relative fluorescence units from triplicate or duplicate wells. Error bars represent ±SD.
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 690 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies horseradish peroxidase conjugated secondary antibody
    A) Induced SR-BI expression in HEK293 cells. Expression of SR-BI was induced by different concentrations of Doxycycline as indicated on the figure. Western blot analysis was performed with anti-SR-BI <t>antibody</t> (Novus Biologicals, NB400-113). <t>Horseradish</t> <t>peroxidase-conjugated</t> <t>secondary</t> antibody and the enhanced chemiluminescent substrate system (Amersham) were used. B) Bodipy-CE uptake is dependent on the expression level of SR-BI. After Doxycycline induction, HEK293[pTRE-tight-SR-BI] cells were incubated with 10 µg/mL DiO-HDL. The selective uptake was determined as described in Experimental Procedures. Data are presented as mean relative fluorescence units from triplicate or duplicate wells. Error bars represent ±SD.
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 967 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Beyotime horseradish peroxidase conjugated secondary antibodies
    A) Induced SR-BI expression in HEK293 cells. Expression of SR-BI was induced by different concentrations of Doxycycline as indicated on the figure. Western blot analysis was performed with anti-SR-BI <t>antibody</t> (Novus Biologicals, NB400-113). <t>Horseradish</t> <t>peroxidase-conjugated</t> <t>secondary</t> antibody and the enhanced chemiluminescent substrate system (Amersham) were used. B) Bodipy-CE uptake is dependent on the expression level of SR-BI. After Doxycycline induction, HEK293[pTRE-tight-SR-BI] cells were incubated with 10 µg/mL DiO-HDL. The selective uptake was determined as described in Experimental Procedures. Data are presented as mean relative fluorescence units from triplicate or duplicate wells. Error bars represent ±SD.
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 400 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Boehringer Mannheim horseradish peroxidase conjugated secondary antibodies
    A) Induced SR-BI expression in HEK293 cells. Expression of SR-BI was induced by different concentrations of Doxycycline as indicated on the figure. Western blot analysis was performed with anti-SR-BI <t>antibody</t> (Novus Biologicals, NB400-113). <t>Horseradish</t> <t>peroxidase-conjugated</t> <t>secondary</t> antibody and the enhanced chemiluminescent substrate system (Amersham) were used. B) Bodipy-CE uptake is dependent on the expression level of SR-BI. After Doxycycline induction, HEK293[pTRE-tight-SR-BI] cells were incubated with 10 µg/mL DiO-HDL. The selective uptake was determined as described in Experimental Procedures. Data are presented as mean relative fluorescence units from triplicate or duplicate wells. Error bars represent ±SD.
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Jackson Laboratory horseradish peroxidase conjugated secondary antibodies
    A) Induced SR-BI expression in HEK293 cells. Expression of SR-BI was induced by different concentrations of Doxycycline as indicated on the figure. Western blot analysis was performed with anti-SR-BI <t>antibody</t> (Novus Biologicals, NB400-113). <t>Horseradish</t> <t>peroxidase-conjugated</t> <t>secondary</t> antibody and the enhanced chemiluminescent substrate system (Amersham) were used. B) Bodipy-CE uptake is dependent on the expression level of SR-BI. After Doxycycline induction, HEK293[pTRE-tight-SR-BI] cells were incubated with 10 µg/mL DiO-HDL. The selective uptake was determined as described in Experimental Procedures. Data are presented as mean relative fluorescence units from triplicate or duplicate wells. Error bars represent ±SD.
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 92/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology horseradish peroxidase conjugated secondary antibody
    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 3205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abbkine horseradish peroxidase conjugated secondary antibodies
    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by Abbkine, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SouthernBiotech horseradish peroxidase conjugated secondary antibodies
    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 92/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biotrend Chemicals horseradish peroxidase conjugated secondary antibody
    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Biotrend Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GeneTex horseradish peroxidase conjugated secondary antibodies
    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vazyme Biotech Co horseradish peroxidase conjugated secondary antibody
    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 91/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioss horseradish peroxidase conjugated secondary antibodies
    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pharmingen horseradish peroxidase conjugated secondary antibodies
    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by Pharmingen, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Rockland Immunochemicals horseradish peroxidase conjugated secondary antibodies
    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech horseradish peroxidase conjugated secondary antibody
    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Full-length suPAR increases adhesion of KG1 cells to fibronectin Panel A: KG1 cells were resuspended in 1mg/ml BSA-RPMI at a density of 1×10 6 cells/ml, pre-incubated with or without suPAR (20 nM), uPAR 84-95 or scrambled peptide (10 nM) for 30′ at 37°C and plated on plastic-bound fibronectin for 16 h at 4°C. Attached cells were fixed with 3% PFA and stained with crystal violet; stain was eluted and its absorbance at 540 nm was measured by a spectrophotometer. (*) p≤0.05, as determined by the Student's t test. Panel B: suPAR-treated or untreated KG1 cells were fixed with PFA, incubated with antibodies against total or active β1 integrin or with nonimmune mouse Ig and, then, with FITC anti-mouse IgG. Cells were finally analyzed by flow cytometry using a FACScan (MFI: Mean Fluorescence Intensity). (*) p≤0.05, as determined by the Student's t test. Panel C: Cell surface antigens of suPAR-treated or untreated KG1 cells were biotinylated with EZ-link sulfo-NHS-LC-biotin. Cells were lysed; 50 μg of cell lysates were analyzed by Western blot with an antibody against total β1 integrin, 400 μg of cell lysates were incubated with an antibody against active β1 integrin or nonimmune mouse Ig. Immunocomplexes were then precipitated by protein A-Sepharose, eluted and analyzed by Western blot with horseradish peroxidase-conjugated streptavidin.

    Journal: Oncotarget

    Article Title: Involvement of urokinase receptor in the cross-talk between human hematopoietic stem cells and bone marrow microenvironment

    doi: 10.18632/oncotarget.11115

    Figure Lengend Snippet: Full-length suPAR increases adhesion of KG1 cells to fibronectin Panel A: KG1 cells were resuspended in 1mg/ml BSA-RPMI at a density of 1×10 6 cells/ml, pre-incubated with or without suPAR (20 nM), uPAR 84-95 or scrambled peptide (10 nM) for 30′ at 37°C and plated on plastic-bound fibronectin for 16 h at 4°C. Attached cells were fixed with 3% PFA and stained with crystal violet; stain was eluted and its absorbance at 540 nm was measured by a spectrophotometer. (*) p≤0.05, as determined by the Student's t test. Panel B: suPAR-treated or untreated KG1 cells were fixed with PFA, incubated with antibodies against total or active β1 integrin or with nonimmune mouse Ig and, then, with FITC anti-mouse IgG. Cells were finally analyzed by flow cytometry using a FACScan (MFI: Mean Fluorescence Intensity). (*) p≤0.05, as determined by the Student's t test. Panel C: Cell surface antigens of suPAR-treated or untreated KG1 cells were biotinylated with EZ-link sulfo-NHS-LC-biotin. Cells were lysed; 50 μg of cell lysates were analyzed by Western blot with an antibody against total β1 integrin, 400 μg of cell lysates were incubated with an antibody against active β1 integrin or nonimmune mouse Ig. Immunocomplexes were then precipitated by protein A-Sepharose, eluted and analyzed by Western blot with horseradish peroxidase-conjugated streptavidin.

    Article Snippet: Polyclonal anti-caspase3 and anti-VN antibodies, the monoclonal anti-PARP1 and anti-β1 integrin antibodies were purchased from Santa Cruz (Santa Cruz, CA); horseradish peroxidase-conjugated secondary antibodies from Bio-Rad (Hercules, CA) and FITC anti-mouse IgG from Jackson Lab. (West Grove, PA).

    Techniques: Incubation, Staining, Spectrophotometry, Flow Cytometry, Cytometry, Fluorescence, Western Blot

    A) Induced SR-BI expression in HEK293 cells. Expression of SR-BI was induced by different concentrations of Doxycycline as indicated on the figure. Western blot analysis was performed with anti-SR-BI antibody (Novus Biologicals, NB400-113). Horseradish peroxidase-conjugated secondary antibody and the enhanced chemiluminescent substrate system (Amersham) were used. B) Bodipy-CE uptake is dependent on the expression level of SR-BI. After Doxycycline induction, HEK293[pTRE-tight-SR-BI] cells were incubated with 10 µg/mL DiO-HDL. The selective uptake was determined as described in Experimental Procedures. Data are presented as mean relative fluorescence units from triplicate or duplicate wells. Error bars represent ±SD.

    Journal: International Journal of Biological Sciences

    Article Title: An apoA-I mimetic peptide facilitates off-loading cholesterol from HDL to liver cells through scavenger receptor BI

    doi:

    Figure Lengend Snippet: A) Induced SR-BI expression in HEK293 cells. Expression of SR-BI was induced by different concentrations of Doxycycline as indicated on the figure. Western blot analysis was performed with anti-SR-BI antibody (Novus Biologicals, NB400-113). Horseradish peroxidase-conjugated secondary antibody and the enhanced chemiluminescent substrate system (Amersham) were used. B) Bodipy-CE uptake is dependent on the expression level of SR-BI. After Doxycycline induction, HEK293[pTRE-tight-SR-BI] cells were incubated with 10 µg/mL DiO-HDL. The selective uptake was determined as described in Experimental Procedures. Data are presented as mean relative fluorescence units from triplicate or duplicate wells. Error bars represent ±SD.

    Article Snippet: Horseradish peroxidase-conjugated secondary antibody and the enhanced chemiluminescent substrate system (Amersham) were used as a detection system.

    Techniques: Expressing, Western Blot, Incubation, Fluorescence

    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by horseradish peroxidase-conjugated goat anti-mouse IgG as secondary antibody (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).

    Journal: Proteome Science

    Article Title: Proteomic analysis of primary duck hepatocytes infected with duck hepatitis B virus

    doi: 10.1186/1477-5956-8-28

    Figure Lengend Snippet: Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by horseradish peroxidase-conjugated goat anti-mouse IgG as secondary antibody (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).

    Article Snippet: The blots were then washed four times with PBS containing 0.1% Tween-20, and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology) 1 hour at room temperature.

    Techniques: Western Blot, Infection, SDS Page, Staining