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Image Search Results
Journal: NPJ precision oncology
Article Title: CD57-positive CD8 + T cells define the response to anti-programmed cell death protein-1 immunotherapy in patients with advanced non-small cell lung cancer.
doi: 10.1038/s41698-024-00513-0
Figure Lengend Snippet: Fig. 4 mIHC/IF-based quantification of CD57+CD8+ T cells predicts immunotherapeutic response in a retrospective NSCLC cohort (n = 90). a Representative images of NSCLC tissue stained using mIHC/IF [CD3 (green), CD8 (red), CD57 (pink), DAPI (blue)]. b mIHC/IF-based CD57+CD8+ T cells/T cells ratio (p < 0.001) and (c) CD57+CD8+ T cells/CD8+ T cells ratio (p < 0.001) predicted immunotherapeutic response in a retrospective NSCLC cohort (n = 90). d Total CD8+ T cells/T cells ratio by mIHC/IF was not of predictive value to immunotherapy (p = 0.679, n = 90). e Spearman’s rank correlation analysis was performed to compare the CD57+CD8+ T cells/T cells ratio (r = 0.644, p < 0.001), and f CD8+ T cells/T cells ratio (r = 0.346, p = 0.016) in blood and tumor samples (n = 48). ***p < 0.001. mIHC/IF multiplex immunohistochemistry/ immunofluorescence, NSCLC non-small cell lung cancer.
Article Snippet: Briefly, each FFPE tissue section was baked at 65 °C for 1 h. After deparaffinization, rehydration, and microwave antigen repair, the slides were blocked (Akoya Biosciences, USA), and incubated with primary
Techniques: Staining, Multiplex Assay, Immunohistochemistry