Journal: Scientific Reports

Article Title: A common genetic variation of melanoma inhibitory activity-2 labels a subtype of pancreatic adenocarcinoma with high endoplasmic reticulum stress levels

doi: 10.1038/srep08109

Figure Lengend Snippet: (a), Pancreatic islet cells are immunopositive for HNF1A and MIA2 (scale bar: 100 μm). (b), 73% and 52% of the samples are positive for HNF1A and MIA2, respectively (scale bar: 100 μm). (c), Protein expression of HNF1A and MIA2 in pancreatic cancer cell lines. Out of 7 tested cell lines (Aspc-1 (As), Bxpc-3 (Bx), Colo357 (Co), Mia-PaCa2 (Mi), Panc-1 (Pa), T3M4 (T3), Su86.86 (Su)), Aspc-1 and Colo-357 express HNF1A and MIA2; endogenous MIA2 is mainly detected at a size of 70 kDa (loading control: β-actin). (d), MRNA expression of MIA2 in 7 pancreatic cancer cell lines. (e), MIA2 silencing with two sets of specific siRNAs (#1 and #2) or negative control siRNA (control) at 72 h in Aspc-1 cells and Colo-357 cells; results of densitometry are shown on top of the blot. One representative blot out of three independent experiments is shown. (f), Transient expression of HNF1A in Su86.86 and Panc-1 cells by transfection of an HNF1A expression vector (upper panel), loading control: GAPDH; MIA2 mRNA expression is induced by transient expression of HNF1A in Su86.86 and Panc-1 cells. HPRT1: housekeeping gene. One of three independent experiments is shown. The gels were run under the same experimental conditions and cropped blots/gels are presented (full-length blots/gels are provided in with indicated cropping lines).

Article Snippet: For transient expression of HNF1A, the expression vector from Origene was used (RC211201, Origene, Suite 200 Rockville, MD).

Techniques: Expressing, Control, Negative Control, Transfection, Plasmid Preparation