Journal: Journal of Biomedical Optics

Article Title: Functionalized erythrocyte-derived optical nanoparticles to target ephrin-B2 ligands

doi: 10.1117/1.JBO.24.8.085002

Figure Lengend Snippet: Ephrin-B2 Western blot expression. Western blot showing the expression of ephrin-B2 in hDMVECs infected with an ephrin-B2 lentivirus compared to the ephrin-B2 expression level of hDMVECs not infected with the ephrin-B2 lentivirus. Beta-actin levels are shown as the internal loading control.

Article Snippet: Human dermal microvascular endothelial cells (hDMVECs) were cultured in endothelial cell basal medium (ECBM) with growth supplement (Cell Applications, Inc.) as previously described.

Techniques: Western Blot, Expressing, Infection, Control

Journal: Journal of Biomedical Optics

Article Title: Functionalized erythrocyte-derived optical nanoparticles to target ephrin-B2 ligands

doi: 10.1117/1.JBO.24.8.085002

Figure Lengend Snippet: Cellular fluorescence of control and ephrin-B2 hDMVECs incubated with F-NETs at various ρ * values. (a) Fluorescent images of control and ephbin-B2 hDMVECs after 40 min of incubation at 4°C with F-NETs solutions. All images are falsely colored with the blue and red corresponding to DAPI and ICG NIR emission from the NETs, respectively. Scale bars are 30 μ m . (b) Averaged fluorescence intensity ( I ¯ ) [see Eq. (3)] as a function of ρ * . Cells from 3 to 4 images were analyzed, resulting in 16 to 41 measurements for each combination of nanoparticles and cells. Statistical significance of p < 0.001 is denoted by ***. Only statistically significant populations with the same ρ * value of F-NETs are indicated. (c) Sigmoidal fit to the I ¯ values of the ephrin-B2 hDMVECs versus the ρ * value of F-NETs. Error bars in (b) and (c) represent SDs.

Article Snippet: Human dermal microvascular endothelial cells (hDMVECs) were cultured in endothelial cell basal medium (ECBM) with growth supplement (Cell Applications, Inc.) as previously described.

Techniques: Fluorescence, Control, Incubation

Journal: Mediators of Inflammation

Article Title: Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation

doi: 10.1155/2009/146872

Figure Lengend Snippet: Dose dependent responses of HUVEC, HCAEC, and HMVEC to IL-1 β (0–2000 pg/mL) as measured by ELISA of released IL-6 protein levels from cell supernatants. Results are expressed as a mean of three separate experiments.

Article Snippet: HUVEC, HCAEC, and HMVEC were purchased from Cambrex BioScience (Walkesville, Maryland, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Mediators of Inflammation

Article Title: Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation

doi: 10.1155/2009/146872

Figure Lengend Snippet: Released IL-6 protein levels as measured by ELISA of supernatants from HUVEC, HCAEC, and HMVEC incubated with IL-1 β (1000 pg/mL) for 24 hours in the absence or presence of BTE (40 μ g/mL) and RSV (40 μ M). Data represent the mean ± SD of three separate experiments. * P < .05 compared with IL-1 β levels of expression.

Article Snippet: HUVEC, HCAEC, and HMVEC were purchased from Cambrex BioScience (Walkesville, Maryland, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Expressing

Journal: Mediators of Inflammation

Article Title: Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation

doi: 10.1155/2009/146872

Figure Lengend Snippet: (a) TF activity was measured in the indicated treatments. Data are presented as arbitrary units, which indicate fold-change above background TF levels and were generated from the mean of three separate experiments. (b) mRNA expressions of β -actin, IL-6, and TF are shown from HUVEC, HCAEC, and HMVEC incubated with IL-1 β (1000 pg/mL) in the absence or presence of BTE (40 μ g/mL) and RSV (40 μ M). The treatments of cell cultures were background control (lane 1), IL-1 β 1000 pg/mL (lane 2), IL-1 β + BTE (lane 3), IL-1 β + RSV (lane 4), BTE (lane 5), and RSV (lane 6). Results shown are from one representative experiment of two separate ones performed.

Article Snippet: HUVEC, HCAEC, and HMVEC were purchased from Cambrex BioScience (Walkesville, Maryland, USA).

Techniques: Activity Assay, Generated, Incubation, Control

Journal: Mediators of Inflammation

Article Title: Increased Responsiveness of Human Coronary Artery Endothelial Cells in Inflammation and Coagulation

doi: 10.1155/2009/146872

Figure Lengend Snippet: (a) IL-1 β dose-dependent prostacyclin release, as measured by 6 keto-PGF 1 α , from HUVEC, HCAEC, and HMVEC. Concentrations of IL-1 β were 0–2000 pg/mL. Data represent the mean of three separate experiments. (b) PGF 1 α levels measured in the indicated endothelial cell types treated with IL-1 β (1000 pg/mL) and/or BTE (40 μ g/mL) [left panel] or IL-1 β (1000 pg/mL) and/or RSV (40 μ M) [right panel]. Data represent the mean ± SD of three separate experiments.

Article Snippet: HUVEC, HCAEC, and HMVEC were purchased from Cambrex BioScience (Walkesville, Maryland, USA).

Techniques: