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  • 86
    Millipore hmscs
    Secretome analysis of preA-adipocytes differentiated from <t>hMSCs.</t> ( A ) Schematic overview of hMSCs cell culture, induction of <t>prelamin</t> A accumulation by TPV treatment, adipogenesis, obtaining CM from hMSCs-derived adipocytes, and subsequent secretome analysis by antibody arrays and LC-MS approaches. Before adipogenic differentiation, induction of prelamin A accumulation in hMSCs was confirmed by confocal microscopy, red: prelamin A, blue: DAPI. Scale bar = 10 µm. (B) Functional annotation clustering of differentially secreted proteins in CM from preA-adipocytes, determined using the DAVID bioinformatic tool. The representative GO terms, grouped in clusters with an enrichment score of 7 or above are presented. The x-axis represents the significance (p value) for each term, while the y-axis represents the ontology categories. (C) Venn diagrams showing overlap of 27 proteins between differentially secreted proteins by preA-hMSCs and preA-adipocytes. Gene ontology analysis of these proteins revealed significant over-representation of categories related to extracellular matrix, collagen binding and cell adhesion. ( D ) Six days after osteogenic differentiation of normal hMSCs in the presence of preA-adipocytes-CM or ctrl-adipocytes-CM, ALP activity was assessed. Results are expressed in reference to ALP activity of hMSCs cultured under ctrl-adipocytes-CM and represent mean ± SD, n = 6.
    Hmscs, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmscs/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hmscs - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    hmscs  (Lonza)
    86
    Lonza hmscs
    Secretome analysis of preA-adipocytes differentiated from <t>hMSCs.</t> ( A ) Schematic overview of hMSCs cell culture, induction of <t>prelamin</t> A accumulation by TPV treatment, adipogenesis, obtaining CM from hMSCs-derived adipocytes, and subsequent secretome analysis by antibody arrays and LC-MS approaches. Before adipogenic differentiation, induction of prelamin A accumulation in hMSCs was confirmed by confocal microscopy, red: prelamin A, blue: DAPI. Scale bar = 10 µm. (B) Functional annotation clustering of differentially secreted proteins in CM from preA-adipocytes, determined using the DAVID bioinformatic tool. The representative GO terms, grouped in clusters with an enrichment score of 7 or above are presented. The x-axis represents the significance (p value) for each term, while the y-axis represents the ontology categories. (C) Venn diagrams showing overlap of 27 proteins between differentially secreted proteins by preA-hMSCs and preA-adipocytes. Gene ontology analysis of these proteins revealed significant over-representation of categories related to extracellular matrix, collagen binding and cell adhesion. ( D ) Six days after osteogenic differentiation of normal hMSCs in the presence of preA-adipocytes-CM or ctrl-adipocytes-CM, ALP activity was assessed. Results are expressed in reference to ALP activity of hMSCs cultured under ctrl-adipocytes-CM and represent mean ± SD, n = 6.
    Hmscs, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmscs/product/Lonza
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hmscs - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    99
    Lonza human mscs
    Secretome analysis of preA-adipocytes differentiated from <t>hMSCs.</t> ( A ) Schematic overview of hMSCs cell culture, induction of <t>prelamin</t> A accumulation by TPV treatment, adipogenesis, obtaining CM from hMSCs-derived adipocytes, and subsequent secretome analysis by antibody arrays and LC-MS approaches. Before adipogenic differentiation, induction of prelamin A accumulation in hMSCs was confirmed by confocal microscopy, red: prelamin A, blue: DAPI. Scale bar = 10 µm. (B) Functional annotation clustering of differentially secreted proteins in CM from preA-adipocytes, determined using the DAVID bioinformatic tool. The representative GO terms, grouped in clusters with an enrichment score of 7 or above are presented. The x-axis represents the significance (p value) for each term, while the y-axis represents the ontology categories. (C) Venn diagrams showing overlap of 27 proteins between differentially secreted proteins by preA-hMSCs and preA-adipocytes. Gene ontology analysis of these proteins revealed significant over-representation of categories related to extracellular matrix, collagen binding and cell adhesion. ( D ) Six days after osteogenic differentiation of normal hMSCs in the presence of preA-adipocytes-CM or ctrl-adipocytes-CM, ALP activity was assessed. Results are expressed in reference to ALP activity of hMSCs cultured under ctrl-adipocytes-CM and represent mean ± SD, n = 6.
    Human Mscs, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human mscs/product/Lonza
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human mscs - by Bioz Stars, 2021-09
    99/100 stars
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    Image Search Results


    Secretome analysis of preA-adipocytes differentiated from hMSCs. ( A ) Schematic overview of hMSCs cell culture, induction of prelamin A accumulation by TPV treatment, adipogenesis, obtaining CM from hMSCs-derived adipocytes, and subsequent secretome analysis by antibody arrays and LC-MS approaches. Before adipogenic differentiation, induction of prelamin A accumulation in hMSCs was confirmed by confocal microscopy, red: prelamin A, blue: DAPI. Scale bar = 10 µm. (B) Functional annotation clustering of differentially secreted proteins in CM from preA-adipocytes, determined using the DAVID bioinformatic tool. The representative GO terms, grouped in clusters with an enrichment score of 7 or above are presented. The x-axis represents the significance (p value) for each term, while the y-axis represents the ontology categories. (C) Venn diagrams showing overlap of 27 proteins between differentially secreted proteins by preA-hMSCs and preA-adipocytes. Gene ontology analysis of these proteins revealed significant over-representation of categories related to extracellular matrix, collagen binding and cell adhesion. ( D ) Six days after osteogenic differentiation of normal hMSCs in the presence of preA-adipocytes-CM or ctrl-adipocytes-CM, ALP activity was assessed. Results are expressed in reference to ALP activity of hMSCs cultured under ctrl-adipocytes-CM and represent mean ± SD, n = 6.

    Journal: Scientific Reports

    Article Title: Secretome analysis of in vitro aged human mesenchymal stem cells reveals IGFBP7 as a putative factor for promoting osteogenesis

    doi: 10.1038/s41598-018-22855-z

    Figure Lengend Snippet: Secretome analysis of preA-adipocytes differentiated from hMSCs. ( A ) Schematic overview of hMSCs cell culture, induction of prelamin A accumulation by TPV treatment, adipogenesis, obtaining CM from hMSCs-derived adipocytes, and subsequent secretome analysis by antibody arrays and LC-MS approaches. Before adipogenic differentiation, induction of prelamin A accumulation in hMSCs was confirmed by confocal microscopy, red: prelamin A, blue: DAPI. Scale bar = 10 µm. (B) Functional annotation clustering of differentially secreted proteins in CM from preA-adipocytes, determined using the DAVID bioinformatic tool. The representative GO terms, grouped in clusters with an enrichment score of 7 or above are presented. The x-axis represents the significance (p value) for each term, while the y-axis represents the ontology categories. (C) Venn diagrams showing overlap of 27 proteins between differentially secreted proteins by preA-hMSCs and preA-adipocytes. Gene ontology analysis of these proteins revealed significant over-representation of categories related to extracellular matrix, collagen binding and cell adhesion. ( D ) Six days after osteogenic differentiation of normal hMSCs in the presence of preA-adipocytes-CM or ctrl-adipocytes-CM, ALP activity was assessed. Results are expressed in reference to ALP activity of hMSCs cultured under ctrl-adipocytes-CM and represent mean ± SD, n = 6.

    Article Snippet: For prelamin A accumulation detection, hMSCs or adipocytes derived from hMSCs were grown on glass coverslips and fixed in paraformaldehyde 4% (Sigma, Cat N°: HT501128).

    Techniques: Cell Culture, Derivative Assay, Liquid Chromatography with Mass Spectroscopy, Confocal Microscopy, Functional Assay, Significance Assay, Binding Assay, ALP Assay, Activity Assay

    Analysis of preA-hMSCs-CM reveals altered secretion of proteins related to extracellular matrix, cell adhesion, angiogenesis and wound healing. ( A ) Schematic overview of hMSCs treatment to induce prelamin A accumulation. From each hMSCs line hMSCs accumulating prelamin A (preA-hMSCs) and control hMSCs (ctrl-hMSCs) were obtained in parallel. Prelamin A accumulation at the nuclear envelope was confirmed by confocal microscopy (red: prelamin A, blue: DAPI). Scale bar = 10 µm. Conditioned media from preA-hMSCs and ctrl-hMSCs were collected and subjected to proteomic analysis. Two independent hMSCs lines were use to obtain conditioned media in the case of antibody arrays (n = 2) and 4 independent hMSCs lines in the case of LC-MS (n = 4). ( B ) The differentially secreted proteins by preA-hMSCs (detected by antibody arrays and LC-MS) were interrogated in terms of functional annotation by DAVID Bioinformatics Resources. The representative Gene Ontology terms, grouped in clusters with an enrichment score of 1.5 or above are presented. The x-axis represents the significance (p value) for each term, while the y-axis represents the ontology categories. ( C ) Real-time quantitative PCR was used to assess the expression of Runx2 in pre-hMSCs and ctrl-hMSCs. Runx2 mRNA expression was normalized to the control gene Gapdh and fold induction was then calculated in reference to ctrl-hMSCs. Results are expressed as mean ± SEM (n = 4).

    Journal: Scientific Reports

    Article Title: Secretome analysis of in vitro aged human mesenchymal stem cells reveals IGFBP7 as a putative factor for promoting osteogenesis

    doi: 10.1038/s41598-018-22855-z

    Figure Lengend Snippet: Analysis of preA-hMSCs-CM reveals altered secretion of proteins related to extracellular matrix, cell adhesion, angiogenesis and wound healing. ( A ) Schematic overview of hMSCs treatment to induce prelamin A accumulation. From each hMSCs line hMSCs accumulating prelamin A (preA-hMSCs) and control hMSCs (ctrl-hMSCs) were obtained in parallel. Prelamin A accumulation at the nuclear envelope was confirmed by confocal microscopy (red: prelamin A, blue: DAPI). Scale bar = 10 µm. Conditioned media from preA-hMSCs and ctrl-hMSCs were collected and subjected to proteomic analysis. Two independent hMSCs lines were use to obtain conditioned media in the case of antibody arrays (n = 2) and 4 independent hMSCs lines in the case of LC-MS (n = 4). ( B ) The differentially secreted proteins by preA-hMSCs (detected by antibody arrays and LC-MS) were interrogated in terms of functional annotation by DAVID Bioinformatics Resources. The representative Gene Ontology terms, grouped in clusters with an enrichment score of 1.5 or above are presented. The x-axis represents the significance (p value) for each term, while the y-axis represents the ontology categories. ( C ) Real-time quantitative PCR was used to assess the expression of Runx2 in pre-hMSCs and ctrl-hMSCs. Runx2 mRNA expression was normalized to the control gene Gapdh and fold induction was then calculated in reference to ctrl-hMSCs. Results are expressed as mean ± SEM (n = 4).

    Article Snippet: For prelamin A accumulation detection, hMSCs or adipocytes derived from hMSCs were grown on glass coverslips and fixed in paraformaldehyde 4% (Sigma, Cat N°: HT501128).

    Techniques: Confocal Microscopy, Liquid Chromatography with Mass Spectroscopy, Functional Assay, Significance Assay, Real-time Polymerase Chain Reaction, Expressing