Journal: Respiratory Research
Article Title: Immunohistological features related to functional impairment in lymphangioleiomyomatosis
Figure Lengend Snippet: Representative histological images of H E staining and immunohistochemistry for protein expression (stained in brown) in each group. All immunohistological parameters showed significantly higher values in the more severe group. a , c , e , g , i , and k : the less severe group; b , d , f , h , j , and l : the more severe group. A and B: H E staining showing the distribution of cysts and LAM-cells nodules (arrows) in each group (magnification: 5×). c and d : SMA expression in LAM-cells (magnification: C, 7× and D, 7×). e and f : HMB-45 expression in LAM-cells (magnification: E, 20× and F, 40×). g and h : VEGF-D expression in LAM-cells (magnification: G, 100× and H, 100×). i and j : MMP-9 expression in LAM-cells (magnification: I, 25× and J, 25×). k and l : mTOR expression in LAM-cells (magnification: K, 35× and L, 35×)
Article Snippet: Sections were incubated with the primary antibody overnight at 4 °C using the following antibodies: SMA, clone 1A4, 1:1000, Dako, California, United States; HMB-45, clone HMB-45, 1:400, Dako, California, United States; VEGF-D, clone 78,923, 1:7000, R & D Systems, United Kingdom.
Techniques: Staining, Immunohistochemistry, Expressing, Laser Capture Microdissection