Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: HMBPP analog prodrugs bypass energy-dependent uptake to promote efficient BTN3A1-mediated malignant cell lysis by Vγ9Vδ2 T lymphocyte effectors

doi: 10.4049/jimmunol.1501833

Figure Lengend Snippet: A) Lysis assay results of WT, BTN3A1 WT clone 4, and BTN3A1 deficient clones 17, 18 and 26. K562 cells were pre-loaded for 2 hours with 100 nM HMBPP or 100 nM POM2-C-HMBP. B/C) Quantification of lysis assays shown in panel A. D) Lysis assay results of WT, clone 26 and clone 26+BTN3A1 K562 cells pre-treated for 2 hours with 100 nM test compound at 37°C followed by a 4 hour co-incubation with effector cells. E) Quantification of lysis assay described in D, where there is a significant rescue of lysis in clone 26 when expressing myc-tagged BTN3A1. F) Flow diagrams of K562 cell lysis induced by pre-treatment of T effector cells with TCR antibody. G) Quantification of panel F. Flow plots are representative of greater than three independent experiments. *, significance between treatments or groups identified.

Article Snippet: RPMI-1640, FBS, non-essential amino acids, pyruvate, penicillin streptomycin, HEPES, 2-ME, HMBPP and FITC-γδ TCR antibody (clone 5A6.E91) were obtained through Fisher Scientific.

Techniques: Lysis, Clone Assay, Incubation, Expressing

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: HMBPP analog prodrugs bypass energy-dependent uptake to promote efficient BTN3A1-mediated malignant cell lysis by Vγ9Vδ2 T lymphocyte effectors

doi: 10.4049/jimmunol.1501833

Figure Lengend Snippet: Phosphoantigens such as HMBPP are internalized by an energy dependent process, which can be bypassed by POM2-C-HMBP. Both BTN3A1 and the Vγ9Vδ2 TCR are required for T cell activation in response to these phosphoantigens. It is unknown how BTN3A1 activation may trigger a T cell response, but this could occur either through direct TCR engagement or through a co-stimulatory process.

Article Snippet: RPMI-1640, FBS, non-essential amino acids, pyruvate, penicillin streptomycin, HEPES, 2-ME, HMBPP and FITC-γδ TCR antibody (clone 5A6.E91) were obtained through Fisher Scientific.

Techniques: Activation Assay

Journal: STAR Protocols

Article Title: Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells

doi: 10.1016/j.xpro.2022.101422

Figure Lengend Snippet:

Article Snippet: HMBPP (HDMAPP ammonium salt) , Cayman Chemical , Cat#13580.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software, Stripping Membranes, Microscopy