Journal: Signal Transduction and Targeted Therapy

Article Title: Anti-lymphangiogenesis for boosting drug accumulation in tumors

doi: 10.1038/s41392-024-01794-4

Figure Lengend Snippet: Anti-lymphangiogenesis activity of anlotinib and SAR131675 both in vitro and in vivo. a – c Anlotinib inhibited the proliferation ( a ), migration ( b ), and tubule network formation ( c ) of hLECs ( n = 4). Scale bar, 200 μm in ( b ) and 1000 μm in ( c ). d – f SAR131675 inhibited the proliferation ( d ), migration ( e ), and tubule network formation ( f ) of hLECs ( n = 4). Scale bar, 200 μm in ( e ) and 1000 μm in ( f ). g Quantification of the recovered area by hLECs migration as shown in ( b ) ( n = 3). h Quantification of the recovered area by hLECs migration as shown in and ( e ) ( n = 3). i Quantification of tube lengths as shown in ( c ) ( n = 3). j Quantification of tube lengths as shown in ( f ) ( n = 3). k Immunohistochemistry staining for LVs (LYVE-1, brown) in 4T1 tumor sections from mice treated with saline, anlotinib or SAR131675. Scale bar, 40 μm. l Quantification of tumor LV density ( n = 9; images were from three mice per group). m Typical images of inguinal LNs from different groups. Scale bar, 0.5 cm. The data are presented as the mean ± s.d. * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: The human lymphatic endothelial cell line (hLECs) was purchased from iCell, China.

Techniques: Activity Assay, In Vitro, In Vivo, Migration, Immunohistochemistry, Staining, Saline

Journal: iScience

Article Title: Autocrine and paracrine effects of MDK promote lymph node metastasis of cervical squamous cell carcinoma

doi: 10.1016/j.isci.2024.110077

Figure Lengend Snippet:

Article Snippet: HLECs cells , Sciencell , 2500.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, ATP Assay, Western Blot, RNA Sequencing, Expressing, Plasmid Preparation, Software, Microscopy, SYBR Green Assay, Flow Cytometry