hla-abc Search Results


anti hla abc  (Miltenyi Biotec)
94
Miltenyi Biotec anti hla abc
Anti Hla Abc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
anti hla abc - by Bioz Stars, 2026-03
94/100 stars
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mhc  (Bio-Techne corporation)
91
Bio-Techne corporation mhc
Neuroblastoma <t>tumor</t> <t>organoids</t> are killed by TEG002 in the presence of Pamidronate, independent of <t>MHC-I</t> expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.
Mhc, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mhc/product/Bio-Techne corporation
Average 91 stars, based on 1 article reviews
mhc - by Bioz Stars, 2026-03
91/100 stars
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fitc conjugated antihuman hla abc  (Bio-Rad)
93
Bio-Rad fitc conjugated antihuman hla abc
Neuroblastoma <t>tumor</t> <t>organoids</t> are killed by TEG002 in the presence of Pamidronate, independent of <t>MHC-I</t> expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.
Fitc Conjugated Antihuman Hla Abc, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc conjugated antihuman hla abc/product/Bio-Rad
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fitc conjugated antihuman hla abc - by Bioz Stars, 2026-03
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mouse anti human hla abc w6 32 conjugated to alexafluor647  (Bio-Rad)
93
Bio-Rad mouse anti human hla abc w6 32 conjugated to alexafluor647
Neuroblastoma <t>tumor</t> <t>organoids</t> are killed by TEG002 in the presence of Pamidronate, independent of <t>MHC-I</t> expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.
Mouse Anti Human Hla Abc W6 32 Conjugated To Alexafluor647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti human hla abc w6 32 conjugated to alexafluor647/product/Bio-Rad
Average 93 stars, based on 1 article reviews
mouse anti human hla abc w6 32 conjugated to alexafluor647 - by Bioz Stars, 2026-03
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hla abc  (Miltenyi Biotec)
94
Miltenyi Biotec hla abc
Neuroblastoma <t>tumor</t> <t>organoids</t> are killed by TEG002 in the presence of Pamidronate, independent of <t>MHC-I</t> expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.
Hla Abc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hla abc/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
hla abc - by Bioz Stars, 2026-03
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rat anti human monoclonal antibody against comp  (Bio-Rad)
93
Bio-Rad rat anti human monoclonal antibody against comp
Neuroblastoma <t>tumor</t> <t>organoids</t> are killed by TEG002 in the presence of Pamidronate, independent of <t>MHC-I</t> expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.
Rat Anti Human Monoclonal Antibody Against Comp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti human monoclonal antibody against comp/product/Bio-Rad
Average 93 stars, based on 1 article reviews
rat anti human monoclonal antibody against comp - by Bioz Stars, 2026-03
93/100 stars
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anti human pe hla abc  (Miltenyi Biotec)
94
Miltenyi Biotec anti human pe hla abc
Neuroblastoma <t>tumor</t> <t>organoids</t> are killed by TEG002 in the presence of Pamidronate, independent of <t>MHC-I</t> expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.
Anti Human Pe Hla Abc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti human pe hla abc/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
anti human pe hla abc - by Bioz Stars, 2026-03
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hla abc viobright515  (Miltenyi Biotec)
94
Miltenyi Biotec hla abc viobright515
Neuroblastoma <t>tumor</t> <t>organoids</t> are killed by TEG002 in the presence of Pamidronate, independent of <t>MHC-I</t> expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.
Hla Abc Viobright515, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hla abc viobright515/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
hla abc viobright515 - by Bioz Stars, 2026-03
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anti hla abc 144nd  (fluidigm)
90
fluidigm anti hla abc 144nd
Neuroblastoma <t>tumor</t> <t>organoids</t> are killed by TEG002 in the presence of Pamidronate, independent of <t>MHC-I</t> expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.
Anti Hla Abc 144nd, supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti hla abc 144nd - by Bioz Stars, 2026-03
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igg1 fitc hla dr dq dp  (Miltenyi Biotec)
94
Miltenyi Biotec igg1 fitc hla dr dq dp
Neuroblastoma <t>tumor</t> <t>organoids</t> are killed by TEG002 in the presence of Pamidronate, independent of <t>MHC-I</t> expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.
Igg1 Fitc Hla Dr Dq Dp, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/igg1 fitc hla dr dq dp/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
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hla abc pe vio770 130 120 433 miltenyi biotec  (Miltenyi Biotec)
94
Miltenyi Biotec hla abc pe vio770 130 120 433 miltenyi biotec
Neuroblastoma <t>tumor</t> <t>organoids</t> are killed by TEG002 in the presence of Pamidronate, independent of <t>MHC-I</t> expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.
Hla Abc Pe Vio770 130 120 433 Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hla abc pe vio770 130 120 433 miltenyi biotec/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
hla abc pe vio770 130 120 433 miltenyi biotec - by Bioz Stars, 2026-03
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hla e abc vioblue  (Miltenyi Biotec)
94
Miltenyi Biotec hla e abc vioblue
Neuroblastoma <t>tumor</t> <t>organoids</t> are killed by TEG002 in the presence of Pamidronate, independent of <t>MHC-I</t> expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.
Hla E Abc Vioblue, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Neuroblastoma tumor organoids are killed by TEG002 in the presence of Pamidronate, independent of MHC-I expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.

Journal: Journal of Personalized Medicine

Article Title: αβ-T Cells Engineered to Express γδ-T Cell Receptors Can Kill Neuroblastoma Organoids Independent of MHC-I Expression

doi: 10.3390/jpm11090923

Figure Lengend Snippet: Neuroblastoma tumor organoids are killed by TEG002 in the presence of Pamidronate, independent of MHC-I expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.

Article Snippet: For two organoids, the cytotoxicity and MHC-I independent recognition was tested in the presence of 10 μg/mL MHC-I blocking antibody (clone W6.32, Cat. NB100-64775, BioTechne, Minneapolis, MN, USA) or 10 μg/mL IgG isotype control (Cat. 31903, ThermoFisher, Waltham, MA, USA).

Techniques: Expressing, Flow Cytometry, Activation Assay, Lysis, Co-Culture Assay, Fluorescence, Comparison, Blocking Assay