Journal: Journal of translational medicine

Article Title: Cold atmospheric plasma drives USP49/HDAC3 axis mediated ferroptosis as a novel therapeutic strategy in endometrial cancer via reinforcing lactylation dependent p53 expression.

doi: 10.1186/s12967-025-06449-8

Figure Lengend Snippet: Fig. 9 CAP suppresses the advancement of endometrial cancer by triggering ferroptosis in vivo.(A) Chart depicting the process of nude mice xenograft assay. (B, C) At the conclusion of the study, the mice were euthanized and dissected. Tumor growth and weight were then assessed. (D) The tumor vol umes in the nude mice were assessed at 3-day intervals. (E) Images of H&E stained hearts, livers, spleens, and kidneys in each group (20x). (F) Representa tive heart, liver, spleen, and kidney images. (G) Western blot was used to detect ACSL3, SLC7A11, FTH1, GPX4, USP49, HDAC3, p53, and H3K18la protein expression levels. (H, I) Representative H&E staining images of tumor tissue, alongside IHC staining images for USP49, HDAC3, H3K18la, p53, Ki-67, ACSL3, SLC7A11, FTH1, and GPX4 at a magnification of 20×. The scale bar represents 100 μm. Data are shown as means ± standard deviation (SD). *: p < 0.05; **: p < 0.01; ***: p < 0.001

Article Snippet: The antibodies against HDAC3 (Cat.NO.CY5595, Abways, China), anti-USP49 (Cat.NO.18066-1-AP, Proteintech, China), anti-L- Lactyl Lysine (Cat. NO.PTM-1401, PTM BIO, China), Anti-Lactyl-Histone H3 (Lys18) (Cat.NO.PTM-1406 and PTM-1427RM, PTM BIO, China), anti-Acetyl-Histone H3 (Lys18) (Cat.NO.PTM-114RM, PTM BIO, China), AntiAcetyllysine (Cat.NO.PTM105RM, PTMBIO, China), anti-p53 (Cat.NO.104421AP, Proteintech, China), anti-ACSL3 (Cat.NO.YN0828, Immunoway, USA), anti-SLC7A11 (Cat.NO.CY7046, Abways, China), antiFTH1 (Cat.NO.CY7085, Abways, China), anti-GPX4 (Cat.NO.67763-1-Ig, Proteintech, China), anti-Rabbit IgG (Cat.No.30000-0-AP, Proteintech, China), antimouse IgG (Cat.No.B900620, Proteintech, China), anti-Histone3 (Cat.NO.CY6587, Abways, China), anti-GAPDH (Cat.NO.10494-1-AP, Proteintech, China), anti-HA (Cat.No.81290-1-RR, Proteintech, China), anti-MYC (Cat.No.16286-1-AP, Proteintech, China), HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) ( Cat.No.SA00001-2, Proteintech, China), HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) (Cat. No.SA00001-1, Proteintech, China).

Techniques: In Vivo, Xenograft Assay, Staining, Western Blot, Expressing, Immunohistochemistry, Standard Deviation

Journal: Cell reports

Article Title: Janus Kinase 1 Plays a Critical Role in Mammary Cancer Progression

doi: 10.1016/j.celrep.2018.10.063

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal, MyD88 , Bosterbio , Cat# PA1600.

Techniques: Recombinant, Sample Prep, Software

Journal: Translational Neuroscience

Article Title: Effect of C-phycocyanin on HDAC3 and miRNA-335 in Alzheimer’s disease

doi: 10.1515/tnsci-2020-0101

Figure Lengend Snippet: CPC treatment alters HDAC3, BDNF, and miRNA-335 expressions in the Aβ-challenged mice. (a) Representative bands of HDAC3 and BDNF expressions with β-actin as an internal control. (b) Densitometric assessment of HDAC3/β-actin and BDNF/β-actin, represented as % of control. (c) Indicative RT-PCR image of miRNA-335 expression in various animal groups. (d) Relative miRNA-335 expression level in each group. Data are indicated as mean ± S.D. * P < 0.05 (Aβ compared with sham); # P < 0.05 (CPC plus Aβ compared with Aβ alone). (e) Shown are representative images of BDNF and HDAC3 expressions in various animal groups. (f) Quantitative assessment of BDNF and HDAC3 expressions, represented as % of control.

Article Snippet: Subsequently, the sections were incubated overnight at 4°C with primary antibodies BDNF (1:500; Boster Bio, USA) and HDAC3 (1:500; Boster Bio, USA) in a humidified compartment.

Techniques: Control, Reverse Transcription Polymerase Chain Reaction, Expressing