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ADInstruments
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Carl Zeiss
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Carl Zeiss
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Carl Zeiss
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GraphPad Software Inc
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Verlag GmbH
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Schmid GmbH
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GraphPad Software Inc
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GraphPad Software Inc
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HighRes Biosolutions
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GraphPad Software Inc
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CyFlo Ltd
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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Small-Molecule Cyclophilin Inhibitors Potently Reduce Platelet Procoagulant Activity
doi: 10.3390/ijms24087163
Figure Lengend Snippet: Small-molecule cyclophilin inhibitors inhibit coagulation induced by procoagulant platelets. ( A ) Whole blood was incubated with 125 µM F759 or F83236, 4 µM CsA before perfusion over a Horm collagen-coated surface in the presence of 6.6 mM CaCl 2 and 3.08 mM MgCl 2 . Shown are representative images after 9 min of top row: DIC images of platelet aggregates; middle row: anti-GpIb-VhH Alexa647 indicating platelets and bottom row: tPA-Alexa488 binding to fibrin. ( B ) Quantification of Δ MFI anti-GpIb-VhH Alexa647 signal during 9 min. ( C ) MFI tPA-Alexa488 over time. ( D ) Quantification of Δ MFI tPA-Alexa488 signal during 9 min. Data represented as mean ± SD of 5–7 separate experiments. * p < 0.05 compared to vehicle by One-Way ANOVA. SMCypIs, small-molecule cyclophilin inhibitors; CsA, Cyclosporin A; tPA, tissue plasminogen activator.
Article Snippet: Images were analyzed for the formation of fibrin via
Techniques: Coagulation, Incubation, Binding Assay