hiseq 2500 Illumina Inc Search Results


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  • 99
    Illumina Inc hiseq 2500 system
    MiRNA expressions profiles of exosomes. (A) Profiling of small RNAs in exosome samples. (B) A Venn diagram showing the co-expressed and specifically expressed miRNAs in exosomes. (C) Heat map of sequencing results. Gene expression data obtained using next-generation sequencing on the Illumina <t>HiSeq</t> 2500 platform. P
    Hiseq 2500 System, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 7521 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 system/product/Illumina Inc
    Average 99 stars, based on 7521 article reviews
    Price from $9.99 to $1999.99
    hiseq 2500 system - by Bioz Stars, 2020-02
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    91
    Illumina Inc hiseq 2000 2500 sequencer
    MiRNA expressions profiles of exosomes. (A) Profiling of small RNAs in exosome samples. (B) A Venn diagram showing the co-expressed and specifically expressed miRNAs in exosomes. (C) Heat map of sequencing results. Gene expression data obtained using next-generation sequencing on the Illumina <t>HiSeq</t> 2500 platform. P
    Hiseq 2000 2500 Sequencer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 2500 sequencer/product/Illumina Inc
    Average 91 stars, based on 50 article reviews
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    hiseq 2000 2500 sequencer - by Bioz Stars, 2020-02
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    88
    Illumina Inc hiseq 2500 rapid flowcells
    MiRNA expressions profiles of exosomes. (A) Profiling of small RNAs in exosome samples. (B) A Venn diagram showing the co-expressed and specifically expressed miRNAs in exosomes. (C) Heat map of sequencing results. Gene expression data obtained using next-generation sequencing on the Illumina <t>HiSeq</t> 2500 platform. P
    Hiseq 2500 Rapid Flowcells, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 rapid flowcells/product/Illumina Inc
    Average 88 stars, based on 21 article reviews
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    hiseq 2500 rapid flowcells - by Bioz Stars, 2020-02
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    79
    Illumina Inc hiseq 2000 2500 instrumentation
    Flow chart for GBS and filtering of the C2-50 and Riesling SNP sets. Genomic DNA was isolated from the parents and F 1 progeny genotypes. Genome complexity was reduced by digesting the genomic DNA with the Ape KI methylation sensitive restriction endonuclease. Libraries were sequenced using <t>Illumina</t> HiSeq 2000/2500 and sequence reads were aligned to the PN40024 reference genome [ 40 , 41 ] using BWA [ 42 ]. After using the TASSEL V3.0.166 SNP calling pipeline [ 39 ], a 509,293 SNP set was generated. The called SNPs were filtered using VCFtools [ 43 ] with a depth of read (DP) > 10, minor allele frequency (MAF) > 0.2, missing data (MD) = 1, and a genotype quality score (GQ) > 98. This filtering step reduced the SNP set to 18,124. SNPs were parsed using a pseudo test cross strategy [ 44 ]. The C2-50 and Riesling SNP sets contained 3974 and 2973 SNPs, respectively.
    Hiseq 2000 2500 Instrumentation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 79/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 2500 instrumentation/product/Illumina Inc
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    hiseq 2000 2500 instrumentation - by Bioz Stars, 2020-02
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    99
    Illumina Inc hiseq 2000 2500 platform
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Hiseq 2000 2500 Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hiseq 2000 2500 platform - by Bioz Stars, 2020-02
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    81
    Illumina Inc hiseq 2500 illumina
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Hiseq 2500 Illumina, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 81/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hiseq 2500 illumina - by Bioz Stars, 2020-02
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    79
    Illumina Inc hisequation 2500 machine
    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.
    Hisequation 2500 Machine, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hisequation 2500 machine/product/Illumina Inc
    Average 79 stars, based on 5 article reviews
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    hisequation 2500 machine - by Bioz Stars, 2020-02
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    99
    Illumina Inc hiseq 2500 sequencing system
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hiseq 2500 Sequencing System, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1290 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 sequencing system/product/Illumina Inc
    Average 99 stars, based on 1290 article reviews
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    hiseq 2500 sequencing system - by Bioz Stars, 2020-02
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    88
    Illumina Inc 50se hiseq 2500
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    50se Hiseq 2500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 14 article reviews
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    50se hiseq 2500 - by Bioz Stars, 2020-02
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    91
    Illumina Inc hiseq 2000 2500 system
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hiseq 2000 2500 System, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 2500 system/product/Illumina Inc
    Average 91 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 2500 system - by Bioz Stars, 2020-02
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    86
    Illumina Inc hiseq 2500 sequence analyzer
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hiseq 2500 Sequence Analyzer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 sequence analyzer/product/Illumina Inc
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    hiseq 2500 sequence analyzer - by Bioz Stars, 2020-02
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    78
    Illumina Inc hiseq 1500 2500 platform
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hiseq 1500 2500 Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 1500 2500 platform/product/Illumina Inc
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    hiseq 1500 2500 platform - by Bioz Stars, 2020-02
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    78
    Illumina Inc hiseq 2500 v4 system
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hiseq 2500 V4 System, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 v4 system/product/Illumina Inc
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    hiseq 2500 v4 system - by Bioz Stars, 2020-02
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    99
    Illumina Inc paired end illumina
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Paired End Illumina, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    paired end illumina - by Bioz Stars, 2020-02
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    80
    Illumina Inc hiseq 2500 300bp paired ends
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hiseq 2500 300bp Paired Ends, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 300bp paired ends/product/Illumina Inc
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    hiseq 2500 300bp paired ends - by Bioz Stars, 2020-02
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    78
    Illumina Inc multiplexed hiseq 2500
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Multiplexed Hiseq 2500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    multiplexed hiseq 2500 - by Bioz Stars, 2020-02
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    88
    Illumina Inc hiseq 2500 4000 system
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hiseq 2500 4000 System, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 4000 system/product/Illumina Inc
    Average 88 stars, based on 18 article reviews
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    hiseq 2500 4000 system - by Bioz Stars, 2020-02
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    92
    Illumina Inc hisequation 2500 platform
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hisequation 2500 Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hisequation 2500 platform/product/Illumina Inc
    Average 92 stars, based on 74 article reviews
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    hisequation 2500 platform - by Bioz Stars, 2020-02
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    99
    Illumina Inc hiseq sequencing platform
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hiseq Sequencing Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 859 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq sequencing platform/product/Illumina Inc
    Average 99 stars, based on 859 article reviews
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    hiseq sequencing platform - by Bioz Stars, 2020-02
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    90
    Illumina Inc hiseq 2500 rapid run platform
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hiseq 2500 Rapid Run Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 rapid run platform/product/Illumina Inc
    Average 90 stars, based on 27 article reviews
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    hiseq 2500 rapid run platform - by Bioz Stars, 2020-02
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    94
    Illumina Inc hiseq 2500 rapid run
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hiseq 2500 Rapid Run, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 101 article reviews
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    82
    Illumina Inc hiseq 2500 genome sequencing system
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hiseq 2500 Genome Sequencing System, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2500 genome sequencing system/product/Illumina Inc
    Average 82 stars, based on 6 article reviews
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    79
    Illumina Inc hiseq model 2500 sequencer
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hiseq Model 2500 Sequencer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hiseq model 2500 sequencer - by Bioz Stars, 2020-02
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    84
    Illumina Inc sample multiplexed sequencing
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Sample Multiplexed Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 84/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 84 stars, based on 12 article reviews
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    88
    Illumina Inc hisequation 2500
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Hisequation 2500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hisequation 2500/product/Illumina Inc
    Average 88 stars, based on 81 article reviews
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    hisequation 2500 - by Bioz Stars, 2020-02
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    80
    Illumina Inc rapid sbs kit
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
    Rapid Sbs Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 5 article reviews
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    rapid sbs kit - by Bioz Stars, 2020-02
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    78
    Illumina Inc hiseq 2500 ht v3 4
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
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    Illumina Inc hiseq 2500 system guide protocol
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
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    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
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    Illumina Inc 2500 hiseq rapid cluster kit
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
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    Illumina Inc illumina sequencing platform
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
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    Illumina Inc ilumina hiseq 2500
    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina <t>HiSeq-2500</t> sequencer.
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    Image Search Results


    MiRNA expressions profiles of exosomes. (A) Profiling of small RNAs in exosome samples. (B) A Venn diagram showing the co-expressed and specifically expressed miRNAs in exosomes. (C) Heat map of sequencing results. Gene expression data obtained using next-generation sequencing on the Illumina HiSeq 2500 platform. P

    Journal: Translational Oncology

    Article Title: Circulating Exosomal miR-17-5p and miR-92a-3p Predict Pathologic Stage and Grade of Colorectal Cancer

    doi: 10.1016/j.tranon.2017.12.012

    Figure Lengend Snippet: MiRNA expressions profiles of exosomes. (A) Profiling of small RNAs in exosome samples. (B) A Venn diagram showing the co-expressed and specifically expressed miRNAs in exosomes. (C) Heat map of sequencing results. Gene expression data obtained using next-generation sequencing on the Illumina HiSeq 2500 platform. P

    Article Snippet: To investigate the molecular mechanism of primary and metastatic CRC, we compared the miRNA expression profiles of exosomes secreted by two isogenic human CRC cell lines (primary SW480 cell line and its lymph node metastatic variant SW620) using the Illumina HiSeq 2500 system.

    Techniques: Sequencing, Expressing, Next-Generation Sequencing

    Overview of experimental design and analysis pipeline. RNA from pepper leaves subjected to each abiotic stress (heat, cold, salinity, and osmotic stress) and the 0-h sample from the mock control was harvested. Marker gene expression was confirmed for each stress condition, and the values were normalized to C. annuum actin expression and were calculated relative to control group as mean values with standard deviation. The validated RNAs were sequenced by the Illumina HiSeq 2500 system. All RNA-seq reads were preprocessed for a quality assessment. The filtered transcriptome reads were aligned to the CM334 genome, and the expression profile was analyzed.

    Journal: Scientific Data

    Article Title: Transcriptome profiling of abiotic responses to heat, cold, salt, and osmotic stress of Capsicum annuum L.

    doi: 10.1038/s41597-020-0352-7

    Figure Lengend Snippet: Overview of experimental design and analysis pipeline. RNA from pepper leaves subjected to each abiotic stress (heat, cold, salinity, and osmotic stress) and the 0-h sample from the mock control was harvested. Marker gene expression was confirmed for each stress condition, and the values were normalized to C. annuum actin expression and were calculated relative to control group as mean values with standard deviation. The validated RNAs were sequenced by the Illumina HiSeq 2500 system. All RNA-seq reads were preprocessed for a quality assessment. The filtered transcriptome reads were aligned to the CM334 genome, and the expression profile was analyzed.

    Article Snippet: For RNA sequencing, 150-nt, paired-end sequencing was conducted using a HiSeq 2500 platform (Illumina, USA) at Macrogen (Korea).

    Techniques: Marker, Expressing, Standard Deviation, RNA Sequencing Assay

    Zinc-related and gli cluster genes were upregulated during phagocytosis. ( A ) Total RNA was extracted from the macrophage phagocytic cells infected with conidia of A. fumigatus , and libraries were prepared from 2 µg of total RNA. High-throughput sequencing was performed as paired-end 100 bp sequencing using a HiSeq 2500 (Illumina, Inc., USA). mRNA-seq reads were mapped using the TopHat software tool to obtain an alignment file. ( B ) Total RNA was extracted from the phagocytic cells infected with conidia after 0 and 1 h postinfection, and cDNA was synthesized using a TOPscript™ cDNA synthesis kit (Enzynomics). Then, qRT-PCR was performed using KAPA SYBR ® for LightCycler ® 480 with the primer sets for the indicated genes. ( C ) The transcriptomic profile during phagocytosis is shown. The gene expression profile was grouped into zinc, copper, iron, and gliotoxin metabolism. Upregulated genes were found in all categories, and ZafA and GliZ were upregulated during phagocytosis. Up- and downregulated genes are colored to show fold change. * p

    Journal: International Journal of Molecular Sciences

    Article Title: The Role of Zinc in Gliotoxin Biosynthesis of Aspergillus fumigatus

    doi: 10.3390/ijms20246192

    Figure Lengend Snippet: Zinc-related and gli cluster genes were upregulated during phagocytosis. ( A ) Total RNA was extracted from the macrophage phagocytic cells infected with conidia of A. fumigatus , and libraries were prepared from 2 µg of total RNA. High-throughput sequencing was performed as paired-end 100 bp sequencing using a HiSeq 2500 (Illumina, Inc., USA). mRNA-seq reads were mapped using the TopHat software tool to obtain an alignment file. ( B ) Total RNA was extracted from the phagocytic cells infected with conidia after 0 and 1 h postinfection, and cDNA was synthesized using a TOPscript™ cDNA synthesis kit (Enzynomics). Then, qRT-PCR was performed using KAPA SYBR ® for LightCycler ® 480 with the primer sets for the indicated genes. ( C ) The transcriptomic profile during phagocytosis is shown. The gene expression profile was grouped into zinc, copper, iron, and gliotoxin metabolism. Upregulated genes were found in all categories, and ZafA and GliZ were upregulated during phagocytosis. Up- and downregulated genes are colored to show fold change. * p

    Article Snippet: High-throughput sequencing was performed as paired-end 100 bp sequencing using a HiSeq 2500 (Illumina, Inc., California, USA). mRNA-seq reads were mapped using the TopHat software tool to obtain an alignment file.

    Techniques: Infection, Next-Generation Sequencing, Sequencing, Software, Synthesized, Quantitative RT-PCR, Expressing

    Effect of the GC content and ERCC length on the estimation of the ERCC cDNA abundance with the ONT MinION platform. The figures present deviations of the ERCC expression level estimates with the ONT MinION platform from the Ambion RNA molecular counts ( A,C ) or from the Illumina HiSeq 2500/MiSeq estimated cDNA abundance ( B,D ) as a function of the GC content ( A,B ) and the ERCC length ( C,D ). We plot the log2 ratio of observed (ONT MinION) to expected (Ambion, Illumina) read counts for the ERCC spike-ins (y-axis, log) for each of the samples relative to their length or GC content (x-axis). Due to the variable sequencing depth from each ERCC MinION experiment, each point is the average value from different MinION flow cell runs if at least 5 reads have been detected for this point in the corresponding MinION runs. The points are colored differently based on the number of flow cell runs in which they were detected (red, green, cyan, purple correspond to values derived from one, two, three or four flow cell runs respectively). The standard deviation is also presented for points with values from two or more MinION runs.

    Journal: Scientific Reports

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations

    doi: 10.1038/srep31602

    Figure Lengend Snippet: Effect of the GC content and ERCC length on the estimation of the ERCC cDNA abundance with the ONT MinION platform. The figures present deviations of the ERCC expression level estimates with the ONT MinION platform from the Ambion RNA molecular counts ( A,C ) or from the Illumina HiSeq 2500/MiSeq estimated cDNA abundance ( B,D ) as a function of the GC content ( A,B ) and the ERCC length ( C,D ). We plot the log2 ratio of observed (ONT MinION) to expected (Ambion, Illumina) read counts for the ERCC spike-ins (y-axis, log) for each of the samples relative to their length or GC content (x-axis). Due to the variable sequencing depth from each ERCC MinION experiment, each point is the average value from different MinION flow cell runs if at least 5 reads have been detected for this point in the corresponding MinION runs. The points are colored differently based on the number of flow cell runs in which they were detected (red, green, cyan, purple correspond to values derived from one, two, three or four flow cell runs respectively). The standard deviation is also presented for points with values from two or more MinION runs.

    Article Snippet: Initially we compared the concordance of the cDNA abundance for the isoforms with length more than 700 bp between the PacBio RS II and Illumina HiSeq 2500 platforms.

    Techniques: Expressing, Sequencing, Flow Cytometry, Derivative Assay, Standard Deviation

    Estimation of the ERCC cDNA abundance with the ONT MinION platform. We compared the ERCC cDNA abundance estimated from the ONT MinION ( A ) and the Illumina HiSeq 2500 or MiSeq platforms ( B ) against the expected number of RNA molecules as provided from the manufacturer (Ambion). The template reads from the ERCC MinION experiments number 1, 2, 3, 4 were pooled together and used for ( A ). Similarly, the corresponding Illumina data were pooled together for ( B ). The Illumina molecular counts data were derived using 5′ molecular tags at the RT step as described in material and methods. The total number of molecules presented on the x-axis corresponds to 3.5 pgs of ERCC RNA. In both axes the log10 transformation of the original count number is used.

    Journal: Scientific Reports

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations

    doi: 10.1038/srep31602

    Figure Lengend Snippet: Estimation of the ERCC cDNA abundance with the ONT MinION platform. We compared the ERCC cDNA abundance estimated from the ONT MinION ( A ) and the Illumina HiSeq 2500 or MiSeq platforms ( B ) against the expected number of RNA molecules as provided from the manufacturer (Ambion). The template reads from the ERCC MinION experiments number 1, 2, 3, 4 were pooled together and used for ( A ). Similarly, the corresponding Illumina data were pooled together for ( B ). The Illumina molecular counts data were derived using 5′ molecular tags at the RT step as described in material and methods. The total number of molecules presented on the x-axis corresponds to 3.5 pgs of ERCC RNA. In both axes the log10 transformation of the original count number is used.

    Article Snippet: Initially we compared the concordance of the cDNA abundance for the isoforms with length more than 700 bp between the PacBio RS II and Illumina HiSeq 2500 platforms.

    Techniques: Derivative Assay, Transformation Assay

    Detection of different cDNA species for the RPL41 gene from either the Illumina HiSeq 2500 platform, the ONT MinION platform or the PacBio RS II platform. ONT MinION reads that were sequenced as full length, as defined by the presence of both the 5′ and 3′ RT adaptors, are presented. For the PacBio RS II example the corresponding “Circular Consensus Sequencing” reads are presented. These reads correspond to fully sequenced molecules. mRNA molecules from GenBank for the RPL41 gene are also shown. For the Illumina data a pileup of the sequenced paired-end fragments is presented.

    Journal: Scientific Reports

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations

    doi: 10.1038/srep31602

    Figure Lengend Snippet: Detection of different cDNA species for the RPL41 gene from either the Illumina HiSeq 2500 platform, the ONT MinION platform or the PacBio RS II platform. ONT MinION reads that were sequenced as full length, as defined by the presence of both the 5′ and 3′ RT adaptors, are presented. For the PacBio RS II example the corresponding “Circular Consensus Sequencing” reads are presented. These reads correspond to fully sequenced molecules. mRNA molecules from GenBank for the RPL41 gene are also shown. For the Illumina data a pileup of the sequenced paired-end fragments is presented.

    Article Snippet: Initially we compared the concordance of the cDNA abundance for the isoforms with length more than 700 bp between the PacBio RS II and Illumina HiSeq 2500 platforms.

    Techniques: Sequencing

    Estimation of the HEK-293 cDNA isoform abundance with three sequencing platforms. The comparison between the cDNA isoform abundance estimated from the Illumina HiSeq 2500 platform and from the PacBio RS II platform is presented in ( A ). The expression level of the HEK-293 isoforms estimated with the ONT MinION platform is compared with the one calculated from either the PacBio RS II ( B ) or the Illumina HiSeq 2500 platform ( C ). For the Illumina HiSeq 2500 platform the expression level, presented as TPM, was estimated with the Sailfish 21 software. For the PacBio RS II or the ONT MinION platform the counts of sequenced molecules per isoform are presented.

    Journal: Scientific Reports

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations

    doi: 10.1038/srep31602

    Figure Lengend Snippet: Estimation of the HEK-293 cDNA isoform abundance with three sequencing platforms. The comparison between the cDNA isoform abundance estimated from the Illumina HiSeq 2500 platform and from the PacBio RS II platform is presented in ( A ). The expression level of the HEK-293 isoforms estimated with the ONT MinION platform is compared with the one calculated from either the PacBio RS II ( B ) or the Illumina HiSeq 2500 platform ( C ). For the Illumina HiSeq 2500 platform the expression level, presented as TPM, was estimated with the Sailfish 21 software. For the PacBio RS II or the ONT MinION platform the counts of sequenced molecules per isoform are presented.

    Article Snippet: Initially we compared the concordance of the cDNA abundance for the isoforms with length more than 700 bp between the PacBio RS II and Illumina HiSeq 2500 platforms.

    Techniques: Sequencing, Expressing, Software

    Flow chart for GBS and filtering of the C2-50 and Riesling SNP sets. Genomic DNA was isolated from the parents and F 1 progeny genotypes. Genome complexity was reduced by digesting the genomic DNA with the Ape KI methylation sensitive restriction endonuclease. Libraries were sequenced using Illumina HiSeq 2000/2500 and sequence reads were aligned to the PN40024 reference genome [ 40 , 41 ] using BWA [ 42 ]. After using the TASSEL V3.0.166 SNP calling pipeline [ 39 ], a 509,293 SNP set was generated. The called SNPs were filtered using VCFtools [ 43 ] with a depth of read (DP) > 10, minor allele frequency (MAF) > 0.2, missing data (MD) = 1, and a genotype quality score (GQ) > 98. This filtering step reduced the SNP set to 18,124. SNPs were parsed using a pseudo test cross strategy [ 44 ]. The C2-50 and Riesling SNP sets contained 3974 and 2973 SNPs, respectively.

    Journal: PLoS ONE

    Article Title: SNP markers tightly linked to root knot nematode resistance in grapevine (Vitis cinerea) identified by a genotyping-by-sequencing approach followed by Sequenom MassARRAY validation

    doi: 10.1371/journal.pone.0193121

    Figure Lengend Snippet: Flow chart for GBS and filtering of the C2-50 and Riesling SNP sets. Genomic DNA was isolated from the parents and F 1 progeny genotypes. Genome complexity was reduced by digesting the genomic DNA with the Ape KI methylation sensitive restriction endonuclease. Libraries were sequenced using Illumina HiSeq 2000/2500 and sequence reads were aligned to the PN40024 reference genome [ 40 , 41 ] using BWA [ 42 ]. After using the TASSEL V3.0.166 SNP calling pipeline [ 39 ], a 509,293 SNP set was generated. The called SNPs were filtered using VCFtools [ 43 ] with a depth of read (DP) > 10, minor allele frequency (MAF) > 0.2, missing data (MD) = 1, and a genotype quality score (GQ) > 98. This filtering step reduced the SNP set to 18,124. SNPs were parsed using a pseudo test cross strategy [ 44 ]. The C2-50 and Riesling SNP sets contained 3974 and 2973 SNPs, respectively.

    Article Snippet: Single-end 100 bp sequence reads were generated using Illumina HiSeq 2000/2500 (Illumina Inc., San Diego, CA, USA).

    Techniques: Flow Cytometry, Isolation, Methylation, Sequencing, Generated

    Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.

    Journal: Molecular Systems Biology

    Article Title: Predominant contribution of cis-regulatory divergence in the evolution of mouse alternative splicing

    doi: 10.15252/msb.20145970

    Figure Lengend Snippet: Study design Fibroblast cells were isolated from adult C57BL/6J, SPRET/EiJ, and the F1 hybrid mice and cultured. PolyA RNAs prepared from each cell line were sequenced on an Illumina HiSeq 2000/2500 platform.

    Article Snippet: Divergence in alternative splicing between C57BL/6J and SPRET/EiJ To characterize the divergence of alternative splicing between C57BL/6J and SPRET/EiJ, we derived fibroblast cell lines from the two mouse strains and sequenced three biological replicates of polyA RNAs isolated from them on an Illumina HiSeq 2000/2500 platform (Fig , Materials and Methods).

    Techniques: Isolation, Mouse Assay, Cell Culture

    Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina HiSeq-2500 sequencer.

    Journal: International Journal of Molecular Sciences

    Article Title: Evaluation and Adaptation of a Laboratory-Based cDNA Library Preparation Protocol for Retrospective Sequencing of Archived MicroRNAs from up to 35-Year-Old Clinical FFPE Specimens

    doi: 10.3390/ijms18030627

    Figure Lengend Snippet: Illustrated representation of the optimized barcoded cDNA library preparation procedure. Total RNA from formalin-fixed paraffin-embedded (FFPE) specimens ( n = 18), are ligated with 3′ adapters ( n = 18), purified on a 15% PAGE gel (using size markers (SM) RNA oligonucleotides) and excised after SYBR ® gold staining of the gel. The combined purified products are ligated with a 5′ adapter, run on a 12% PAGE gel, excised and purified. The dual-adapter ligated small-RNA products are reverse transcribed, subjected to a pilot PCR for identification of adequate amplification cycle. A large-scale PCR is performed, and the products are separated on a 2% agarose gel, where the upper band (cDNA library) is purified and subjected to sequencing on an Illumina HiSeq-2500 sequencer.

    Article Snippet: The cDNA libraries were then sequenced (single-read 50 cycles) on a HiSeq 2500 Sequencing System (SY-401-2501, Illumina, San Diego, CA, USA).

    Techniques: cDNA Library Assay, Formalin-fixed Paraffin-Embedded, Purification, Polyacrylamide Gel Electrophoresis, Staining, Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Sequencing