Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Induction of innervation by encapsulated adipocytes with engineered vitamin A metabolism

doi: 10.1016/j.trsl.2017.10.005

Figure Lengend Snippet: (A) Schematic of the main processes leading to production of retinoic acid (RA) in WAT. ALDH1A1 produces 70% of RA which activates RAR in adipocytes (100%) [20]. NGF plasma levels were examined by ELISA in WT and Aldh1a1−/− mice fed regular chow (B) or HF diet (C), n=4 per group randomly selected from Study 1 and 2. Relative expressions of Rbfox3 (D), Nestin (E), and Synopsin (F) were measured in iAb WAT from WT and Aldh1a1−/− males (triangle, n=5) and females (circle, n=5) by TaqMan RT-PCR (HF, Study 1). Expressions were normalized to Tata box protein (TBP). The levels of peripherin (G) and tyrosine hydroxylase (TH) (H) were analyzed by Western blot from randomly selected tissues of WT (n=4) and Aldh1a1−/− mice (n=4) (HF, Study 1). Protein expression was normalized by levels of housekeeping genes (β-actin). The insert shows representative Western blot. (I) Norepinephrine (NE) level from iAb fat pat homogenates in WT (n=6) and Aldh1a1−/− mice (n=5) was measured using ELISA (HF diet, Study 1). An asterisk indicates a significant difference between WT and Aldh1a1−/− groups (p<0.05). n.s.: not significant, Mann-Whitney U test.

Article Snippet: Norepinephrine (NE) measurements Norepinephrine was measured in plasma and fat samples using Noradrenaline High Sensitive ELISA (EA633.96, Eagle Biosciences/DLD Diagnostika Hamburg, Germany) according to manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, MANN-WHITNEY

Journal: Nature Communications

Article Title: Nanomedicine-based co-delivery of a calcium channel inhibitor and a small molecule targeting CD47 for lung cancer immunotherapy

doi: 10.1038/s41467-023-42972-2

Figure Lengend Snippet: a Schematic illustration of BMDCs stimulation in a transwell system. LLC cells were placed in the upper chamber followed by various treatments for 16 h, then the resultant media containing nanomedicines were removed and BMDCs were cultured in the lower chamber. b Quantifications of the secretion levels of TNF-α, IL-1β, IL-6, and IL-12p70 in the stimulated BMDCs suspensions. The data are expressed as means ± SD ( N = 3 independent experiments). Boxplots show the distribution of expression with the center of the box representing the mean, the center line correspond to the median, upper and lower bounds representing 75% and 25% percentiles. All statistical significances were calculated via one-sided unpaired Student’s t test. c CLSM images of BMDCs after being co-incubated with LLC cells pre-treated with LDH/LR/LT/LRT. The cells were then stained with DiI (red) and DAPI (blue) for lightening plasma membrane and nucleus, respectively. Scale bar, 30 µm. N = 3 samples with similar results. d FACS plots of CD80 and CD86 expressions on BMDCs gated on CD11c + cells, representative of 3 independent experiments. e Flow cytometric counts of CFSE-labeled T cells gated on CD3 + CD8 + cells for T cell proliferation analysis, representative of 3 independent experiments. Source data are provided as a Source Data file.

Article Snippet: The supernatant of stimulated BMDCs was collected, and mouse TNF-α (MULTI SCIENCES, EK282), IL-1β (MULTI SCIENCES, EK201BHS), IL-6 (Bio-Techne Valukine TM , VAL604G) and IL-12p70 (MULTI SCIENCES, EK212HS) ELISA kits quantified the cytokines released into the supernatant with a standard protocol.

Techniques: Cell Culture, Expressing, Incubation, Staining, Clinical Proteomics, Membrane, Labeling