high resolution microarray scanner Search Results


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  • 99
    Thermo Fisher high resolution microarray scanner genechip scanner 3000 7g
    High Resolution Microarray Scanner Genechip Scanner 3000 7g, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 699 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution microarray scanner
    High expressed miRNAs in normal human placenta ( A ) HeatMap representing the top 30 high expressed miRNAs in normal human placenta. ( B ) Boxplot presenting normalized level of expression of the 866 miRNAs evaluated in normal human placenta using <t>microarray</t> miRNA expression profile. The bold line indicates de median of signal intensity of all miRNAs; the top edge represents the 75th percentile and the bottom edge the 25th percentile. Vertical bars indicate the upper and inferior limits and circles represent outlier points/samples (points 1.5 IQR above the 3rd quartile of the boxplot).
    High Resolution Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1502 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution microarray scanner 62505c
    High expressed miRNAs in normal human placenta ( A ) HeatMap representing the top 30 high expressed miRNAs in normal human placenta. ( B ) Boxplot presenting normalized level of expression of the 866 miRNAs evaluated in normal human placenta using <t>microarray</t> miRNA expression profile. The bold line indicates de median of signal intensity of all miRNAs; the top edge represents the 75th percentile and the bottom edge the 25th percentile. Vertical bars indicate the upper and inferior limits and circles represent outlier points/samples (points 1.5 IQR above the 3rd quartile of the boxplot).
    High Resolution Microarray Scanner 62505c, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution fluorescence microarray scanner
    High expressed miRNAs in normal human placenta ( A ) HeatMap representing the top 30 high expressed miRNAs in normal human placenta. ( B ) Boxplot presenting normalized level of expression of the 866 miRNAs evaluated in normal human placenta using <t>microarray</t> miRNA expression profile. The bold line indicates de median of signal intensity of all miRNAs; the top edge represents the 75th percentile and the bottom edge the 25th percentile. Vertical bars indicate the upper and inferior limits and circles represent outlier points/samples (points 1.5 IQR above the 3rd quartile of the boxplot).
    High Resolution Fluorescence Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Molecular Devices LLC genepix 4300a high resolution microarray scanner
    High expressed miRNAs in normal human placenta ( A ) HeatMap representing the top 30 high expressed miRNAs in normal human placenta. ( B ) Boxplot presenting normalized level of expression of the 866 miRNAs evaluated in normal human placenta using <t>microarray</t> miRNA expression profile. The bold line indicates de median of signal intensity of all miRNAs; the top edge represents the 75th percentile and the bottom edge the 25th percentile. Vertical bars indicate the upper and inferior limits and circles represent outlier points/samples (points 1.5 IQR above the 3rd quartile of the boxplot).
    Genepix 4300a High Resolution Microarray Scanner, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 96/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution laser microarray scanner
    High expressed miRNAs in normal human placenta ( A ) HeatMap representing the top 30 high expressed miRNAs in normal human placenta. ( B ) Boxplot presenting normalized level of expression of the 866 miRNAs evaluated in normal human placenta using <t>microarray</t> miRNA expression profile. The bold line indicates de median of signal intensity of all miRNAs; the top edge represents the 75th percentile and the bottom edge the 25th percentile. Vertical bars indicate the upper and inferior limits and circles represent outlier points/samples (points 1.5 IQR above the 3rd quartile of the boxplot).
    High Resolution Laser Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies dna high resolution microarray scanner
    High expressed miRNAs in normal human placenta ( A ) HeatMap representing the top 30 high expressed miRNAs in normal human placenta. ( B ) Boxplot presenting normalized level of expression of the 866 miRNAs evaluated in normal human placenta using <t>microarray</t> miRNA expression profile. The bold line indicates de median of signal intensity of all miRNAs; the top edge represents the 75th percentile and the bottom edge the 25th percentile. Vertical bars indicate the upper and inferior limits and circles represent outlier points/samples (points 1.5 IQR above the 3rd quartile of the boxplot).
    Dna High Resolution Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 1505 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution microarray scanner gs2505 c
    High expressed miRNAs in normal human placenta ( A ) HeatMap representing the top 30 high expressed miRNAs in normal human placenta. ( B ) Boxplot presenting normalized level of expression of the 866 miRNAs evaluated in normal human placenta using <t>microarray</t> miRNA expression profile. The bold line indicates de median of signal intensity of all miRNAs; the top edge represents the 75th percentile and the bottom edge the 25th percentile. Vertical bars indicate the upper and inferior limits and circles represent outlier points/samples (points 1.5 IQR above the 3rd quartile of the boxplot).
    High Resolution Microarray Scanner Gs2505 C, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution microarry scanner
    High expressed miRNAs in normal human placenta ( A ) HeatMap representing the top 30 high expressed miRNAs in normal human placenta. ( B ) Boxplot presenting normalized level of expression of the 866 miRNAs evaluated in normal human placenta using <t>microarray</t> miRNA expression profile. The bold line indicates de median of signal intensity of all miRNAs; the top edge represents the 75th percentile and the bottom edge the 25th percentile. Vertical bars indicate the upper and inferior limits and circles represent outlier points/samples (points 1.5 IQR above the 3rd quartile of the boxplot).
    High Resolution Microarry Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565ca high resolution laser microarray scanner
    High expressed miRNAs in normal human placenta ( A ) HeatMap representing the top 30 high expressed miRNAs in normal human placenta. ( B ) Boxplot presenting normalized level of expression of the 866 miRNAs evaluated in normal human placenta using <t>microarray</t> miRNA expression profile. The bold line indicates de median of signal intensity of all miRNAs; the top edge represents the 75th percentile and the bottom edge the 25th percentile. Vertical bars indicate the upper and inferior limits and circles represent outlier points/samples (points 1.5 IQR above the 3rd quartile of the boxplot).
    G2565ca High Resolution Laser Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies surescan high resolution dna microarray scanner
    Early <t>DNA</t> methylation remodeling persists in CD4 + memory T-cells in vivo . ( A ) Barplot of TET gene expression in primary CD4 + Naïve T-cells (NT), central memory (TCM) and effector memory (TEM) cells. Gene expression of TET1/2/3 was measured by qPCR. ( B ) Barplot of global 5hmC content measured by immuno-dot blot using a 5hmC antibody in primary NT, TCM and TEM cells. ( C ) Heatmap of gene expression in primary NT, TCM and TEM cells showing subset specific gene signatures. Gene expression measured by <t>microarray.</t> ( D ) Unsupervised hierarchical clustering of DNA methylation (5mC) in NT, TCM and TEM cells measured by 450K methylation array. Significance calculated by bootstrap resampling. ( E ) Volcano plot of 5mC changes in memory subsets showing a predominant loss in both TCM and TEM cells. Vertical lines indicate a change of 30%. ( F ) Correlation between 5mC and gene expression in TEM cells calculated using Spearman’s rank correlation coefficient. 5mC measured by 450k array and gene expression measured by microarray. ( G ) 5hmC regions show major loss of 5mC in TEM cells. Background represents a randomly sampled group with the same size of probes in 5hmC peaks. Significance calculated using Fisher’s exact test (rightmost). ( H ) Venn diagram of sites losing 5mC in TEM cells and after 5 days of polarization towards Th1 and Th2. Sites losing 5mC defined as a loss of 20% or 30% 5mC vs. NT for polarization and TEM, respectively, and p
    Surescan High Resolution Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution microarray scanner system
    Predictive performance of expression biomarkers in granulosa and cumulus cells according to <t>microarray</t> data. AUC- area under the curve; GC- granulosa cells; CC- cumulus cells.
    High Resolution Microarray Scanner System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies surescan high resolution microarray scanner
    Predictive performance of expression biomarkers in granulosa and cumulus cells according to <t>microarray</t> data. AUC- area under the curve; GC- granulosa cells; CC- cumulus cells.
    Surescan High Resolution Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution microarray scanner g2505
    Predictive performance of expression biomarkers in granulosa and cumulus cells according to <t>microarray</t> data. AUC- area under the curve; GC- granulosa cells; CC- cumulus cells.
    High Resolution Microarray Scanner G2505, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tecan Systems high resolution fluorescence microarray scanner
    Expression and Phosphorylation of RTKs and downstream signaling molecules in NSCLC Immunoarray technology, Collaborative Enzyme Enhanced Reactive-immunoassay (CEER™), was utilized to determine the level of expression and degree of phosphorylation in tumor cells isolated from specimens collected from NSCLC patients. Schematic assay principle and assay format is shown on the left. Each array contains designated standards and controls; multiple photomultiplier (PMT) settings are utilized to have expanded dynamic range of signal quantitation and signals for clinical samples are reported after normalizing against standards on each slide. Capture antibodies printed on <t>microarray</t> surface in triplicate with two dilutions are indicated (right).
    High Resolution Fluorescence Microarray Scanner, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 89/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565ca high resolution microarray scanner
    Expression and Phosphorylation of RTKs and downstream signaling molecules in NSCLC Immunoarray technology, Collaborative Enzyme Enhanced Reactive-immunoassay (CEER™), was utilized to determine the level of expression and degree of phosphorylation in tumor cells isolated from specimens collected from NSCLC patients. Schematic assay principle and assay format is shown on the left. Each array contains designated standards and controls; multiple photomultiplier (PMT) settings are utilized to have expanded dynamic range of signal quantitation and signals for clinical samples are reported after normalizing against standards on each slide. Capture antibodies printed on <t>microarray</t> surface in triplicate with two dilutions are indicated (right).
    G2565ca High Resolution Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution microarray scanner c model
    Expression and Phosphorylation of RTKs and downstream signaling molecules in NSCLC Immunoarray technology, Collaborative Enzyme Enhanced Reactive-immunoassay (CEER™), was utilized to determine the level of expression and degree of phosphorylation in tumor cells isolated from specimens collected from NSCLC patients. Schematic assay principle and assay format is shown on the left. Each array contains designated standards and controls; multiple photomultiplier (PMT) settings are utilized to have expanded dynamic range of signal quantitation and signals for clinical samples are reported after normalizing against standards on each slide. Capture antibodies printed on <t>microarray</t> surface in triplicate with two dilutions are indicated (right).
    High Resolution Microarray Scanner C Model, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565aa agilent high resolution microarray scanners
    Expression and Phosphorylation of RTKs and downstream signaling molecules in NSCLC Immunoarray technology, Collaborative Enzyme Enhanced Reactive-immunoassay (CEER™), was utilized to determine the level of expression and degree of phosphorylation in tumor cells isolated from specimens collected from NSCLC patients. Schematic assay principle and assay format is shown on the left. Each array contains designated standards and controls; multiple photomultiplier (PMT) settings are utilized to have expanded dynamic range of signal quantitation and signals for clinical samples are reported after normalizing against standards on each slide. Capture antibodies printed on <t>microarray</t> surface in triplicate with two dilutions are indicated (right).
    G2565aa Agilent High Resolution Microarray Scanners, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high resolution confocal microarray scanner
    Schematic overview of <t>microarray</t> workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.
    High Resolution Confocal Microarray Scanner, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution dna microarray scanner g2505c
    Schematic overview of <t>microarray</t> workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.
    High Resolution Dna Microarray Scanner G2505c, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g2565ca high resolution dna microarray scanner
    Schematic overview of <t>microarray</t> workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.
    G2565ca High Resolution Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Molecular Devices LLC axon genepix 4400a high resolution microarray scanner
    Schematic overview of <t>microarray</t> workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.
    Axon Genepix 4400a High Resolution Microarray Scanner, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution agilent dna microarray scanner system
    Schematic overview of <t>microarray</t> workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.
    High Resolution Agilent Dna Microarray Scanner System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution dna microarray scanner model g2565ca
    Schematic overview of <t>microarray</t> workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.
    High Resolution Dna Microarray Scanner Model G2565ca, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies surescan high resolution dna microarray scanner g2505 c
    Schematic overview of <t>microarray</t> workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.
    Surescan High Resolution Dna Microarray Scanner G2505 C, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies high resolution c scanner
    Schematic overview of <t>microarray</t> workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.
    High Resolution C Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 96/100, based on 359 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher genechip scanner 3000 located
    Schematic overview of <t>microarray</t> workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.
    Genechip Scanner 3000 Located, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 883 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies resolution array scanner
    Schematic overview of <t>microarray</t> workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.
    Resolution Array Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies g3 high resolution scanner
    Schematic overview of <t>microarray</t> workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.
    G3 High Resolution Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high resolution scanner
    Schematic overview of <t>microarray</t> workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.
    High Resolution Scanner, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies g2565ca high resolution scanner
    Microarray of Slit2 produced log ratios, expression and classification of genes. ( A ) Cartoon of a chicken embryo electroporation. In green we show the putative plasmid solution with fast green being injected inside the neural tube. And the ± shows the position of the electrodes when giving them the electric pulses necessary for the plasmid to enter cells in the NT. ( B ) Workflow and sample preparation and array processing. For the microarray we processed 6 Total RNA samples (1 Control, 2 experimental in duplicates) for gene expression. RNA quality and concentration was determined on the Agilent Bioanalyzer and NanoDrop spectrophotometer. One-color labeling reactions were prepared using the Agilent Two-Color Protocol (Version 6.6) with 400 ng total RNA input. ( C ) Log ratio values collected after microarray show significantly upregulated genes (red), significantly down regulated genes (green), and not differentially expressed (yellow). Control samples were labeled with Cy5 and Experimental sample labeled with Cy3. RNA Spike A mix was used for Cy3 (experimental) samples and RNA spike B mix was used for all Cy5 (Control) samples and scanned at 3 μM resolution on an Agilent <t>G2565CA</t> High Resolution Scanner. ( D ) Percentage of gene expression upon Slit2 gain of function in microarray. The bar graphed represents 37.3% down-regulation genes and 62.7% of incidents generated up-regulated genes of NCCs Slit2 GOF vs control.
    G2565ca High Resolution Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 72 article reviews
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    Image Search Results


    High expressed miRNAs in normal human placenta ( A ) HeatMap representing the top 30 high expressed miRNAs in normal human placenta. ( B ) Boxplot presenting normalized level of expression of the 866 miRNAs evaluated in normal human placenta using microarray miRNA expression profile. The bold line indicates de median of signal intensity of all miRNAs; the top edge represents the 75th percentile and the bottom edge the 25th percentile. Vertical bars indicate the upper and inferior limits and circles represent outlier points/samples (points 1.5 IQR above the 3rd quartile of the boxplot).

    Journal: Oncotarget

    Article Title: Highly expressed placental miRNAs control key biological processes in human cancer cell lines

    doi: 10.18632/oncotarget.25264

    Figure Lengend Snippet: High expressed miRNAs in normal human placenta ( A ) HeatMap representing the top 30 high expressed miRNAs in normal human placenta. ( B ) Boxplot presenting normalized level of expression of the 866 miRNAs evaluated in normal human placenta using microarray miRNA expression profile. The bold line indicates de median of signal intensity of all miRNAs; the top edge represents the 75th percentile and the bottom edge the 25th percentile. Vertical bars indicate the upper and inferior limits and circles represent outlier points/samples (points 1.5 IQR above the 3rd quartile of the boxplot).

    Article Snippet: Microarray slides were scanned using the High-Resolution Microarray Scanner (Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Expressing, Microarray

    Microarray validation of selected putative detoxification and digestive genes overexpressed in the 3 transcriptomic comparisons. ( A ) Larvae fed upon green olives vs . larvae fed upon artificial diet. ( B ) Larvae fed upon black olives vs. larvae fed upon artificial diet. ( C ) Larvae fed upon green olives vs. larvae fed upon black olives. Error bars represent the standard deviation of four biological replicates. Microarray data are also presented. Contig04596: serine protease; contig03768: serine protease; contig04001: UDP-glucosyltransferase; contig04849: serine protease; contig03604: cytochrome P450; contig04090: serine protease; contig04528: serine protease; contig02711: serine protease; contig03020: N-acetyltransferase; contig01887: peptidase; contig00850: metalloprotease; contig06851: lysozyme; contig04367: serine protease; contig07649: protease.

    Journal: Scientific Reports

    Article Title: Transcriptomic responses of the olive fruit fly Bactrocera oleae and its symbiont Candidatus Erwinia dacicola to olive feeding

    doi: 10.1038/srep42633

    Figure Lengend Snippet: Microarray validation of selected putative detoxification and digestive genes overexpressed in the 3 transcriptomic comparisons. ( A ) Larvae fed upon green olives vs . larvae fed upon artificial diet. ( B ) Larvae fed upon black olives vs. larvae fed upon artificial diet. ( C ) Larvae fed upon green olives vs. larvae fed upon black olives. Error bars represent the standard deviation of four biological replicates. Microarray data are also presented. Contig04596: serine protease; contig03768: serine protease; contig04001: UDP-glucosyltransferase; contig04849: serine protease; contig03604: cytochrome P450; contig04090: serine protease; contig04528: serine protease; contig02711: serine protease; contig03020: N-acetyltransferase; contig01887: peptidase; contig00850: metalloprotease; contig06851: lysozyme; contig04367: serine protease; contig07649: protease.

    Article Snippet: Following hybridization, slides were washed using the Gene Expression Wash Buffer kit (Agilent Technologies) and scanned by an Agilent Microarray High-Resolution Scanner using default settings for 8 × 60 K GE microarrays.

    Techniques: Microarray, Standard Deviation

    Early DNA methylation remodeling persists in CD4 + memory T-cells in vivo . ( A ) Barplot of TET gene expression in primary CD4 + Naïve T-cells (NT), central memory (TCM) and effector memory (TEM) cells. Gene expression of TET1/2/3 was measured by qPCR. ( B ) Barplot of global 5hmC content measured by immuno-dot blot using a 5hmC antibody in primary NT, TCM and TEM cells. ( C ) Heatmap of gene expression in primary NT, TCM and TEM cells showing subset specific gene signatures. Gene expression measured by microarray. ( D ) Unsupervised hierarchical clustering of DNA methylation (5mC) in NT, TCM and TEM cells measured by 450K methylation array. Significance calculated by bootstrap resampling. ( E ) Volcano plot of 5mC changes in memory subsets showing a predominant loss in both TCM and TEM cells. Vertical lines indicate a change of 30%. ( F ) Correlation between 5mC and gene expression in TEM cells calculated using Spearman’s rank correlation coefficient. 5mC measured by 450k array and gene expression measured by microarray. ( G ) 5hmC regions show major loss of 5mC in TEM cells. Background represents a randomly sampled group with the same size of probes in 5hmC peaks. Significance calculated using Fisher’s exact test (rightmost). ( H ) Venn diagram of sites losing 5mC in TEM cells and after 5 days of polarization towards Th1 and Th2. Sites losing 5mC defined as a loss of 20% or 30% 5mC vs. NT for polarization and TEM, respectively, and p

    Journal: Cell reports

    Article Title: 5-Hydroxymethylcytosine Remodeling Precedes Lineage Specification during Differentiation of Human CD4(+) T Cells

    doi: 10.1016/j.celrep.2016.05.091

    Figure Lengend Snippet: Early DNA methylation remodeling persists in CD4 + memory T-cells in vivo . ( A ) Barplot of TET gene expression in primary CD4 + Naïve T-cells (NT), central memory (TCM) and effector memory (TEM) cells. Gene expression of TET1/2/3 was measured by qPCR. ( B ) Barplot of global 5hmC content measured by immuno-dot blot using a 5hmC antibody in primary NT, TCM and TEM cells. ( C ) Heatmap of gene expression in primary NT, TCM and TEM cells showing subset specific gene signatures. Gene expression measured by microarray. ( D ) Unsupervised hierarchical clustering of DNA methylation (5mC) in NT, TCM and TEM cells measured by 450K methylation array. Significance calculated by bootstrap resampling. ( E ) Volcano plot of 5mC changes in memory subsets showing a predominant loss in both TCM and TEM cells. Vertical lines indicate a change of 30%. ( F ) Correlation between 5mC and gene expression in TEM cells calculated using Spearman’s rank correlation coefficient. 5mC measured by 450k array and gene expression measured by microarray. ( G ) 5hmC regions show major loss of 5mC in TEM cells. Background represents a randomly sampled group with the same size of probes in 5hmC peaks. Significance calculated using Fisher’s exact test (rightmost). ( H ) Venn diagram of sites losing 5mC in TEM cells and after 5 days of polarization towards Th1 and Th2. Sites losing 5mC defined as a loss of 20% or 30% 5mC vs. NT for polarization and TEM, respectively, and p

    Article Snippet: For gene expression microarrays, RNA was labeled and amplified using the Low Input Quick Amp Labeling kit (Agilent Technologies), then hybridized onto SurePrint G3 Human Gene Expression 8x60K v2 microarrays (Agilent Technologies) and scanned using a Surescan High Resolution DNA Microarray Scanner (Agilent Technologies).

    Techniques: DNA Methylation Assay, In Vivo, Expressing, Transmission Electron Microscopy, Real-time Polymerase Chain Reaction, Dot Blot, Microarray, Methylation

    Predictive performance of expression biomarkers in granulosa and cumulus cells according to microarray data. AUC- area under the curve; GC- granulosa cells; CC- cumulus cells.

    Journal: PLoS ONE

    Article Title: No Specific Gene Expression Signature in Human Granulosa and Cumulus Cells for Prediction of Oocyte Fertilisation and Embryo Implantation

    doi: 10.1371/journal.pone.0115865

    Figure Lengend Snippet: Predictive performance of expression biomarkers in granulosa and cumulus cells according to microarray data. AUC- area under the curve; GC- granulosa cells; CC- cumulus cells.

    Article Snippet: After hybridization, microarray slides were scanned using Agilent High Resolution Microarray Scanner System, using the manufacturers recommended scanning settings.

    Techniques: Expressing, Microarray

    qPCR validation of microarray data. Comparison of relative mRNA expression of the genes selected for validation of microarray data between non-P and P samples. A) qPCR performed on a set of cumulus cells samples that were previously used in microarray analysis. B) qPCR performed on a novel set of cumulus cells samples. Non-P: fertilized, but not pregnant; P- pregnant.

    Journal: PLoS ONE

    Article Title: No Specific Gene Expression Signature in Human Granulosa and Cumulus Cells for Prediction of Oocyte Fertilisation and Embryo Implantation

    doi: 10.1371/journal.pone.0115865

    Figure Lengend Snippet: qPCR validation of microarray data. Comparison of relative mRNA expression of the genes selected for validation of microarray data between non-P and P samples. A) qPCR performed on a set of cumulus cells samples that were previously used in microarray analysis. B) qPCR performed on a novel set of cumulus cells samples. Non-P: fertilized, but not pregnant; P- pregnant.

    Article Snippet: After hybridization, microarray slides were scanned using Agilent High Resolution Microarray Scanner System, using the manufacturers recommended scanning settings.

    Techniques: Real-time Polymerase Chain Reaction, Microarray, Expressing

    Study flowchart. Microarray analysis was performed on 64 individual cumulus and granulosa cell samples derived from 21 women; the number of the samples in each group (non-F, non-P, P) is presented. In the second stage of the study, qPCR validation was performed on 55 samples derived from 41 women; 25 samples had previously been used for microarray analysis, 30 samples were newly added to the study and derived from 20 new women. 'newly added samples; Non-F—unfertilized; non-P—fertilized, but not pregnant; P—pregnant; GC- granulosa cells; CC- cumulus cells.

    Journal: PLoS ONE

    Article Title: No Specific Gene Expression Signature in Human Granulosa and Cumulus Cells for Prediction of Oocyte Fertilisation and Embryo Implantation

    doi: 10.1371/journal.pone.0115865

    Figure Lengend Snippet: Study flowchart. Microarray analysis was performed on 64 individual cumulus and granulosa cell samples derived from 21 women; the number of the samples in each group (non-F, non-P, P) is presented. In the second stage of the study, qPCR validation was performed on 55 samples derived from 41 women; 25 samples had previously been used for microarray analysis, 30 samples were newly added to the study and derived from 20 new women. 'newly added samples; Non-F—unfertilized; non-P—fertilized, but not pregnant; P—pregnant; GC- granulosa cells; CC- cumulus cells.

    Article Snippet: After hybridization, microarray slides were scanned using Agilent High Resolution Microarray Scanner System, using the manufacturers recommended scanning settings.

    Techniques: Microarray, Derivative Assay, Real-time Polymerase Chain Reaction

    Expression and Phosphorylation of RTKs and downstream signaling molecules in NSCLC Immunoarray technology, Collaborative Enzyme Enhanced Reactive-immunoassay (CEER™), was utilized to determine the level of expression and degree of phosphorylation in tumor cells isolated from specimens collected from NSCLC patients. Schematic assay principle and assay format is shown on the left. Each array contains designated standards and controls; multiple photomultiplier (PMT) settings are utilized to have expanded dynamic range of signal quantitation and signals for clinical samples are reported after normalizing against standards on each slide. Capture antibodies printed on microarray surface in triplicate with two dilutions are indicated (right).

    Journal: Oncotarget

    Article Title: Low EGFR/MET ratio is associated with resistance to EGFR inhibitors in non-small cell lung cancer

    doi:

    Figure Lengend Snippet: Expression and Phosphorylation of RTKs and downstream signaling molecules in NSCLC Immunoarray technology, Collaborative Enzyme Enhanced Reactive-immunoassay (CEER™), was utilized to determine the level of expression and degree of phosphorylation in tumor cells isolated from specimens collected from NSCLC patients. Schematic assay principle and assay format is shown on the left. Each array contains designated standards and controls; multiple photomultiplier (PMT) settings are utilized to have expanded dynamic range of signal quantitation and signals for clinical samples are reported after normalizing against standards on each slide. Capture antibodies printed on microarray surface in triplicate with two dilutions are indicated (right).

    Article Snippet: Slides were washed with TBST, dried and immediately processed on a high-resolution fluorescence microarray scanner (PowerScanner, Tecan).

    Techniques: Expressing, Isolation, Quantitation Assay, Microarray

    Schematic overview of microarray workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.

    Journal: PLoS ONE

    Article Title: Longitudinal Analysis of Serum Autoantibody-Reactivities in Patients with Primary Open Angle Glaucoma and Optic Disc Hemorrhage

    doi: 10.1371/journal.pone.0166813

    Figure Lengend Snippet: Schematic overview of microarray workflow. Shown is the basic procedure of ab-screening. Nitrocellulose-slides with antigens of interest are incubated with patients´sera in order to capture autoabs. For visualization of the resultant antigen-ab complexes, slides are incubated with fluorescence-labeled (F) secondary abs followed by confocal laser scanning. Measured intensities were normalized and semi-quantitative results were further investigated by linear trend and statistical analysis.

    Article Snippet: After vacuum-drying (Speed Vac, Thermo), emitted fluorescence signals were digitized by scanning the slides with a high-resolution confocal microarray scanner (Affymetrix 428TM Array Scanner, Wycombe, UK).

    Techniques: Microarray, Incubation, Fluorescence, Labeling

    Microarray of Slit2 produced log ratios, expression and classification of genes. ( A ) Cartoon of a chicken embryo electroporation. In green we show the putative plasmid solution with fast green being injected inside the neural tube. And the ± shows the position of the electrodes when giving them the electric pulses necessary for the plasmid to enter cells in the NT. ( B ) Workflow and sample preparation and array processing. For the microarray we processed 6 Total RNA samples (1 Control, 2 experimental in duplicates) for gene expression. RNA quality and concentration was determined on the Agilent Bioanalyzer and NanoDrop spectrophotometer. One-color labeling reactions were prepared using the Agilent Two-Color Protocol (Version 6.6) with 400 ng total RNA input. ( C ) Log ratio values collected after microarray show significantly upregulated genes (red), significantly down regulated genes (green), and not differentially expressed (yellow). Control samples were labeled with Cy5 and Experimental sample labeled with Cy3. RNA Spike A mix was used for Cy3 (experimental) samples and RNA spike B mix was used for all Cy5 (Control) samples and scanned at 3 μM resolution on an Agilent G2565CA High Resolution Scanner. ( D ) Percentage of gene expression upon Slit2 gain of function in microarray. The bar graphed represents 37.3% down-regulation genes and 62.7% of incidents generated up-regulated genes of NCCs Slit2 GOF vs control.

    Journal: Gene expression patterns : GEP

    Article Title: Screen for Slit/Robo signaling in trunk neural cells reveals new players

    doi: 10.1016/j.gep.2018.01.002

    Figure Lengend Snippet: Microarray of Slit2 produced log ratios, expression and classification of genes. ( A ) Cartoon of a chicken embryo electroporation. In green we show the putative plasmid solution with fast green being injected inside the neural tube. And the ± shows the position of the electrodes when giving them the electric pulses necessary for the plasmid to enter cells in the NT. ( B ) Workflow and sample preparation and array processing. For the microarray we processed 6 Total RNA samples (1 Control, 2 experimental in duplicates) for gene expression. RNA quality and concentration was determined on the Agilent Bioanalyzer and NanoDrop spectrophotometer. One-color labeling reactions were prepared using the Agilent Two-Color Protocol (Version 6.6) with 400 ng total RNA input. ( C ) Log ratio values collected after microarray show significantly upregulated genes (red), significantly down regulated genes (green), and not differentially expressed (yellow). Control samples were labeled with Cy5 and Experimental sample labeled with Cy3. RNA Spike A mix was used for Cy3 (experimental) samples and RNA spike B mix was used for all Cy5 (Control) samples and scanned at 3 μM resolution on an Agilent G2565CA High Resolution Scanner. ( D ) Percentage of gene expression upon Slit2 gain of function in microarray. The bar graphed represents 37.3% down-regulation genes and 62.7% of incidents generated up-regulated genes of NCCs Slit2 GOF vs control.

    Article Snippet: Next, the labeled cRNA is fragmented and placed on the chicken 4×44K Gene Expression microarray and hybridized at 65 °C for ~17 h. Finally, the arrays are washed and scanned at 3 μM resolution on an Agilent G2565CA High Resolution Scanner.

    Techniques: Microarray, Produced, Expressing, Electroporation, Plasmid Preparation, Injection, Sample Prep, Concentration Assay, Spectrophotometry, Labeling, Generated