Journal: Cell

Article Title: Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity

doi: 10.1016/j.cell.2021.01.037

Figure Lengend Snippet:

Article Snippet: Spectraplate-384 with high protein binding , Perkin Elmer , Cat. No. CUSG83093.

Techniques: Virus, Clinical Proteomics, Infection, Recombinant, Blocking Assay, Transfection, Multiplex Assay, Protein Binding, Expressing, Plasmid Preparation, Software, Sequencing, Real-time Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Secreted autotransporter toxin (Sat) induces cell damage during enteroaggregative Escherichia coli infection

doi: 10.1371/journal.pone.0228959

Figure Lengend Snippet: Culture supernatants of 20 bacterial strains (16 sat+ and 4 sat - strains), grown in DMEM, were tested by indirect ELISA for Sat detection using anti-Sat rabbit serum. The strains DEC/Sat+, EAEC CV323/77 and DAEC FBC114 were used as positive controls, and E . coli HB101 as negative control. All strains were tested in triplicates and at least two independent experiments were performed. The data shown are the absorbance values (mean ± standard error) for each strain from a representative experiment.

Article Snippet: The supernatants of the cultures were obtained after centrifugation at 15,700 × g for 10 min, and used for coating ELISA microplates (Corning Incorporated Costar®, high binding) (USA).

Techniques: Indirect ELISA, Negative Control