Journal: Cancer research
Article Title: Small-Molecule Natural Product Physachenolide C Potentiates Immunotherapy Efficacy by Targeting BET Proteins
Figure Lengend Snippet: PCC sensitizes melanomas and renal carcinomas to TRAIL and/or poly I:C mediated apoptosis. A, Chemical structure of PCC. B-C, Effect of PCC in the presence or absence of TRAIL or poly I:C on cell viability of human melanoma cell lines and renal cancer cells vs normal cells. B, A panel of human melanoma cell lines, a renal tumor (ACHN), and normal human melanocytes, foreskin fibroblasts (HFF) and renal epithelium (HRE) cells were treated with PCC (500 nM) for 3 h followed by human recombinant TRAIL (10 ng/ml) (upper panel) or poly I:C (10 μg/ml) (lower panel). Following an 18 h incubation for TRAIL, and 36–48 h for poly I:C, relative cell numbers assessed by MTS assay. C, IC50 values derived from a titration of PCC in the presence or absence of TRAIL (10 ng/ml) or poly I: C (10 μg/ml). PCC alone has an IC50 >2000 nM for all cell lines.
Article Snippet: Human foreskin fibroblast (HFF) cells were purchased from ATCC (Manassas, VA, USA).
Techniques: Recombinant, Incubation, MTS Assay, Derivative Assay, Titration