Journal: International Journal of Molecular Sciences

Article Title: Effects of Passiflora edulis Leaf Extract on Lipid Accumulation in HepG2 Cells: In Vitro Evidence and Molecular Docking Analysis Involving PPARα and SREBP-1

doi: 10.3390/ijms27073003

Figure Lengend Snippet: Effect of ethanolic extract of P. edulis leaves on viability in HepG2 ( A ) and HFF ( B ) cells at 24 and 48 h. Statistically significant differences between the control and treatment groups: ** p < 0.01; *** p < 0.001.

Article Snippet: HepG2 (purchased from ATCC code HB 8065) and HFF (purchased from Cytion, code 305790) cell lines were maintained and propagated in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) with 25 mM glucose and 2 mM L-glutamine, supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 IU/mL penicillin, 100 μg/mL streptomycin (Gibco), and 1% nonessential amino acids (Gibco).

Techniques: Control

Journal: AIDS Research and Human Retroviruses

Article Title: Purging Exhausted Virus-Specific CD8 T Cell Phenotypes by Somatic Cell Reprogramming

doi: 10.1089/aid.2017.0161

Figure Lengend Snippet: Establishment of B6/129OKSM mouse line. (A) Schematic illustration of B6/129OKSM mouse generation. We first established a DOX-reprogrammable fibroblast-like cell line originated from AinV15 mouse ESCs by transduction of STEM-CCA lentiviral vector encoding four DOX-inducible mouse reprogramming genes (Oct4, Klf4, Sox2, and c-Myc). Chimeric mice were generated by the injection of 8–12 established iPSCs into a B6 mouse blastocyst. The mice were bred with B6 mice to obtain F1 hybrids derived from 129P2/OlaHsd strain (agouti coat). F1 hybrids were then crossbred for >10 generations until we established a stable mouse line carrying a single copy of a reprogramming gene cassette introduced by STEM-CCA lentiviral vector homozygously and enabling induction of ESC-like colony formation from peripheral blood cells with high efficiency in the presence of DOX. DOX: doxycycline. Diff.: differentiation. (B) Fibroblasts were isolated from tail-tip biopsies of chimeric mice and exposed to 1 μg/ml of DOX for 2° iPSC induction. No 2° iPSC formation was seen with no DOX addition. (C) Expression of two pluripotent stem cell markers (Nanog and SSEA1) in 2° iPSCs. (D) Embryoid bodies generated from 2° iPSCs. iPSC, induced pluripotent stem cell.

Article Snippet: AinV15 mouse embryonic stem cells (ESCs) were purchased from American Type Culture Collection (ATCC SCRC-1029, Manassas, VA).

Techniques: Transduction, Plasmid Preparation, Generated, Injection, Derivative Assay, Isolation, Expressing