Journal: iScience

Article Title: miRNA-194-3p represses NF-κB in gliomas to attenuate iPSC genes and proneural to mesenchymal transition

doi: 10.1016/j.isci.2023.108650

Figure Lengend Snippet:

Article Snippet: Taqman gene expression assay probes were used to measure the mRNA levels of 194-1 (Thermo Fisher: Cat# Hs04231530_s1), 194-2 (Thermo Fisher: Cat# Hs04331541_s1), ALDH1A3 (Thermo Fisher: Cat# Hs00167476_m1), CD44 (Thermo Fisher: Cat# Hs05662929_s1), LYN (Thermo Fisher: Cat# Hs01015818_g1), WT1 (Thermo Fisher: Cat# Hs01103751_m1), CD133 (Thermo Fisher: Cat# Hs01009259_m1), Nestin (Thermo Fisher: Cat# Hs04187831_g1), OLIG1 (Thermo Fisher: Cat# Hs00907227_s1), SOX2 (Thermo Fisher: Cat# Hs04234836_s1), klf4 (Thermo Fisher: Cat# Hs00358836_m1), OCT4 (Thermo Fisher: Cat# Hs00999632_g1), FUT4 (Thermo Fisher: Cat# Hs01106466_s1), NCAM1 (Thermo Fisher: Cat# Hs00941830_m1), Nanog (Thermo Fisher: Cat# Hs02387400_g1), TAB2 (Thermo Fisher: Cat# Hs00248373_m1), CMYC (Thermo Fisher: Cat# Hs00153408_m1), DOCK5 (Thermo Fisher: Cat# Hs07287975_m1), FAS (Thermo Fisher: Cat# Hs00236330_m1), HFE (Thermo Fisher: Cat# Hs00373474_m1), LGALS3 (Thermo Fisher: Cat# Hs07288627_m1), MAL (Thermo Fisher: Cat# Hs00707014_s1), RHOF (Thermo Fisher: Cat# Hs00368032_m1), SOWAHA (Thermo Fisher: Cat# Hs00703106_s1), C21orf91 (Thermo Fisher: Cat# Hs05040994_s1), TRIM23 (Thermo Fisher: Cat# Hs01106626_m1).

Techniques: Recombinant, Protease Inhibitor, Transfection, Isolation, cDNA Synthesis, MTT Assay, Extraction, Transcription Factor Assay, Expressing, Plasmid Preparation, Reporter Assay, Staining, Negative Control, shRNA, Software

Journal: Oncotarget

Article Title: Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism

doi: 10.18632/oncotarget.14093

Figure Lengend Snippet: Expression of the HEPH g ene at the mRNA level in breast non-malignant cell line MCF10A, in TICs derived from breast cancer cell lines MCF-7, BT-474, T-47D and ZR-75-30 as well as from prostate cancer cell lines DU-145 and LNCaP has been determined ( A ) together with protein levels in the MCF-7 cell line (CTRL) and MCF-7 derived spheres (SPH) ( B ). Similarly, the expression of the HFE gene at the mRNA ( C ) level as well as protein level ( D ) in TICs is documented. Experiments were performed at least in triplicate, standard error is SEM, p -values lower than 0.05 are denoted with a star and were calculated by the GenEx software using the unpaired t-test and plotted with GraphPad prism software. Number sign denotes statistical significance involving Dun-Bonferroni correction. The protein expression was quantified by the image J software from 2 to 5 independent samples, standard error is SEM, p -values lower than 0.05 are denoted with a star and were calculated and plotted in GraphPad prism, using the unpaired t-test .

Article Snippet: Gels were then separated according to standard procedure at 20 mA per well, washed in 1× towbin buffer and blotted onto a nitrocellulose membrane via Xcell blotting module (Invitrogen) at constant voltage (35 V) for 2 h. Membranes were then blocked with 5% non-fat milk for 1 h, washed and incubated in 5% bovine serum albumin (BSA)/Tris-buffered saline (TBS)/0,1% Tween-20 with primary antibodies against ABCB10 (ThermoScientific #PA5-30468, dil. 1:1000), ACO1 (ThermoScientific #PA5-27824, dil. 1:1000), CYBRD1 (Bioss #bs-8297R, dil. 1:1000), EPAS1 (ThermoScientific #PA116510, dil. 1:1000), GLRX5 (Bioss #bs-13395R, dil. 1:1000), HEPH (Bioss #bs-15458R, dil. 1:1000), HFE (Bioss #bs-12335R, dil. 1:1000), IREB2 (ThermoScientific #PA116544, 1:500), QSOX1 (Sigma #SAB2700031, dil. 1:1000), TFRC (ThermoScientific #13-6800, dil. 1:2500), SLC39A14 (Abcam #ab191199, dil. 1:2000), SLC40A1 (Bioss #bs-4906R, dil. 1:1000) SLC11A2 (Cell Signalling #15083, dil. 1:1000), Ferritin (Abcam #ab75973, dil. 1:1000), Actin (Thermoscientific #MA5-15739-HRP, dil. 1:2000), Tubulin (Abcam #ab4742, dil. 1:5000) overnight.

Techniques: Expressing, Derivative Assay, Software

Journal: PLoS ONE

Article Title: Evaluation of a High Throughput Method for the Detection of Mutations Associated with Thrombosis and Hereditary Hemochromatosis in Brazilian Blood Donors

doi: 10.1371/journal.pone.0125460

Figure Lengend Snippet: Comparison of the results obtained using OpenArray analysis and Light Cycler (LC).

Article Snippet: HFE , S65C , rs1800730 , C___1085599_20.

Techniques: Comparison

Journal: PLoS ONE

Article Title: Evaluation of a High Throughput Method for the Detection of Mutations Associated with Thrombosis and Hereditary Hemochromatosis in Brazilian Blood Donors

doi: 10.1371/journal.pone.0125460

Figure Lengend Snippet: SNPs identification and Assay Part Numbers for OpenArray testing.

Article Snippet: HFE , S65C , rs1800730 , C___1085599_20.

Techniques:

Journal: PLoS ONE

Article Title: Evaluation of a High Throughput Method for the Detection of Mutations Associated with Thrombosis and Hereditary Hemochromatosis in Brazilian Blood Donors

doi: 10.1371/journal.pone.0125460

Figure Lengend Snippet: Genotypic and allelic frequencies observed in the studied population using the OpenArray method.

Article Snippet: HFE , S65C , rs1800730 , C___1085599_20.

Techniques:

Journal: Frontiers in Aging Neuroscience

Article Title: Effects of hemochromatosis and transferrin gene mutations on peripheral iron dyshomeostasis in mild cognitive impairment and Alzheimer's and Parkinson's diseases

doi: 10.3389/fnagi.2013.00037

Figure Lengend Snippet: Genotype and allelic frequencies for HFE (C282Y, H63D) and TF (P589S) mutations in controls, AD, MCI, and PD patients .

Article Snippet: The predesigned SNPs genotyping assays ID are C_1085595_10, C_1085600_10, C_7505275_10 (Applied Biosystems).

Techniques: Mutagenesis