Journal: International Journal of Molecular Sciences

Article Title: Anti-Cancer Effects of Oxygen-Atom-Modified Derivatives of Wasabi Components on Human Leukemia Cells

doi: 10.3390/ijms24076823

Figure Lengend Snippet: Chemical structures of 6-MITC, I7447, and I7557.

Article Snippet: Pure 6-MITC, I7447, and I7557 were purchased from LKT Laboratories (St. Paul, MN, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Anti-Cancer Effects of Oxygen-Atom-Modified Derivatives of Wasabi Components on Human Leukemia Cells

doi: 10.3390/ijms24076823

Figure Lengend Snippet: Effects of I7447, 6-MITC, and I7557 on the viability of K562 and HEL cells. Cell viability was assessed using the trypan blue exclusion assay. K562 and HEL cells were treated with or without 10 μM I7447, 6-MITC, and I7557 for 48 h. ( A ) K562; and ( B ) HEL cells. Data from three separate experiments are expressed as the mean ± standard error of the mean (SEM). N = 3 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Pure 6-MITC, I7447, and I7557 were purchased from LKT Laboratories (St. Paul, MN, USA).

Techniques: Trypan Blue Exclusion Assay

Journal: International Journal of Molecular Sciences

Article Title: Anti-Cancer Effects of Oxygen-Atom-Modified Derivatives of Wasabi Components on Human Leukemia Cells

doi: 10.3390/ijms24076823

Figure Lengend Snippet: Effect of wasabi compounds on viability of parent and imatinib−resistant K562 cells. Cell viability was assessed using the trypan blue exclusion assay. K562 cells were treated with or without 0.2 μM imatinib mesylate for 48 and 72 h. ( A ) I7447; ( B ) 6-MITC; and ( C ) I7557 cells. Data from three separate experiments are expressed as the mean ± standard error of the mean (SEM). N = 3 for each group.

Article Snippet: Pure 6-MITC, I7447, and I7557 were purchased from LKT Laboratories (St. Paul, MN, USA).

Techniques: Trypan Blue Exclusion Assay

Journal: International Journal of Molecular Sciences

Article Title: Anti-Cancer Effects of Oxygen-Atom-Modified Derivatives of Wasabi Components on Human Leukemia Cells

doi: 10.3390/ijms24076823

Figure Lengend Snippet: Cell cycle analysis of K562 and HEL cells treated with 6-MITC, I7447, and I7557. Expression level and representative DNA histogram at the sub-G1 and G2/M phases for K562 ( A , B ) and HEL ( C , D ) cells. Data from three separate experiments are expressed as the mean ± standard error of the mean (SEM). N = 3 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Pure 6-MITC, I7447, and I7557 were purchased from LKT Laboratories (St. Paul, MN, USA).

Techniques: Cell Cycle Assay, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Anti-Cancer Effects of Oxygen-Atom-Modified Derivatives of Wasabi Components on Human Leukemia Cells

doi: 10.3390/ijms24076823

Figure Lengend Snippet: Expression of surface antigens on K562 and HEL cells after exposure to I7447, 6-MITC, and I7557. I7447, 6-MITC, and I7557 were incubated for 48 h and assessed by flow cytometry. ( A ) K562 cells; and ( B ) HEL cells. The expression levels of these surface antigens were analyzed using the CellQuest Pro software.

Article Snippet: Pure 6-MITC, I7447, and I7557 were purchased from LKT Laboratories (St. Paul, MN, USA).

Techniques: Expressing, Incubation, Flow Cytometry, Software

Journal: International Journal of Molecular Sciences

Article Title: Anti-Cancer Effects of Oxygen-Atom-Modified Derivatives of Wasabi Components on Human Leukemia Cells

doi: 10.3390/ijms24076823

Figure Lengend Snippet: Autophagy in I7447-, 6-MITC-, and I7557-treated human K562 cells. After Liu’s staining, images were captured at 1000× magnification using a light microscope: ( A ) K562 cells; ( B ) HEL cells. ( C ) TEM images of K562 cells. The accumulated cytoplasmic vacuoles were identified as autophagosomes (indicated by arrows) with double-layered membrane structures containing remnants of organelles. ( D ) Using flow cytometry, acridine orange staining for acidic vesicular organelles was estimated. The expression levels of acridine orange were elevated by treatment with I7447, 6-MITC, and I7557. ( E ) the autophagy inhibitor E/P (10 μg/mL), further increased the expression levels of acridine orange in the treated cells. ( F ) The expression profile of the autophagy-related proteins was evaluated by immunoblotting after treatment with I7447, 6-MITC, and I7557. Data from three separate experiments are expressed as the mean ± standard error of the mean (SEM). N = 3 for each group. * p < 0.05, *** p < 0.001.

Article Snippet: Pure 6-MITC, I7447, and I7557 were purchased from LKT Laboratories (St. Paul, MN, USA).

Techniques: Staining, Light Microscopy, Membrane, Flow Cytometry, Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Anti-Cancer Effects of Oxygen-Atom-Modified Derivatives of Wasabi Components on Human Leukemia Cells

doi: 10.3390/ijms24076823

Figure Lengend Snippet: Cell viability and cell cycle analysis of K562 cells treated with 6-MITC, I7447, and I7557. 6-MITC, I7447, and I7557 were used to treat K562 cells. 3-methyladenine (3-MA; 2 mM), chloroquine (CQ; 12.5 μM), E64D/pepstatin A (E/P) (10 μM), bafilomycin A1 (BafA1; 20 nM), and rapamycin (40 nM) were used. Cell viability ( A ) and cell cycle ( B ) were assessed using the trypan blue exclusion assay and flow cytometry, respectively. Data from three separate experiments are expressed as the mean ± standard error of the mean (SEM). N = 3 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Pure 6-MITC, I7447, and I7557 were purchased from LKT Laboratories (St. Paul, MN, USA).

Techniques: Cell Cycle Assay, Trypan Blue Exclusion Assay, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Anti-Cancer Effects of Oxygen-Atom-Modified Derivatives of Wasabi Components on Human Leukemia Cells

doi: 10.3390/ijms24076823

Figure Lengend Snippet: Apoptosis induction by I7447 and I7557. Based on flow cytometry analysis following annexin V staining, I7447 (10 μM) and I7557 (10 μM) induced significant apoptosis. ( A ) Representative flow cytometric figures. ( B ) Quantitative data for apoptotic cells with positive annexin V staining. Data from three separate experiments are expressed as the mean ± standard error of the mean (SEM). N = 3 for each group. * p < 0.05.

Article Snippet: Pure 6-MITC, I7447, and I7557 were purchased from LKT Laboratories (St. Paul, MN, USA).

Techniques: Flow Cytometry, Staining

Journal: International Journal of Molecular Sciences

Article Title: Wasabi Component 6-(Methylsulfinyl)hexly Isothiocyanate and Derivatives Improve the Survival of Skin Allografts

doi: 10.3390/ijms23158488

Figure Lengend Snippet: Morphological observation of dendritic cells. CD14+ monocytes were treated with ( a ) control; ( b ) vehicle control DMSO; ( c – e ) I7447; ( f – h ) 6-MITC; or ( i – k ) I7557 for 7 days to generate dendritic cells. Cells were stained by Liu’s method for morphological examination by light microscopy (magnification 1000×). As the concentration of 6-MITC, I7457, and I7557 increased, the dendrites of the cells were reduced and shorter.

Article Snippet: The compounds 6-MITC, I7447, and I7557 were obtained from LKT Laboratories (St. Paul, MN, USA), dissolved in dimethyl sulfoxide (DMSO) (Merck, Darmstadt, Germany) as a stock solution, and stored at −20 °C until use.

Techniques: Control, Staining, Light Microscopy, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Wasabi Component 6-(Methylsulfinyl)hexly Isothiocyanate and Derivatives Improve the Survival of Skin Allografts

doi: 10.3390/ijms23158488

Figure Lengend Snippet: 6-MITC, I7447, and I7557 did not significantly affect the viability of dendritic cells at 1, 3.3, and 10 μM.

Article Snippet: The compounds 6-MITC, I7447, and I7557 were obtained from LKT Laboratories (St. Paul, MN, USA), dissolved in dimethyl sulfoxide (DMSO) (Merck, Darmstadt, Germany) as a stock solution, and stored at −20 °C until use.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Wasabi Component 6-(Methylsulfinyl)hexly Isothiocyanate and Derivatives Improve the Survival of Skin Allografts

doi: 10.3390/ijms23158488

Figure Lengend Snippet: Effect of 6-MITC and its derivatives on the expression of the dendritic cell surface markers, CD1a and CD83. DCs were treated with 6-MITC/I7447/I7557 at concentrations of 0, 1, 3.3, and 10 μM for 7 days. CD1a ( a ), CD83 ( b ), CD14 ( c ), and HLA-DR ( d ) expression was assessed by flow cytometry. ( a , b ) The 6-MITC and I7557 exhibited a dual effect on the CD1a and CD83 expression. * p < 0.05 in comparison to control.

Article Snippet: The compounds 6-MITC, I7447, and I7557 were obtained from LKT Laboratories (St. Paul, MN, USA), dissolved in dimethyl sulfoxide (DMSO) (Merck, Darmstadt, Germany) as a stock solution, and stored at −20 °C until use.

Techniques: Expressing, Flow Cytometry, Comparison, Control