Journal: Molecular Cell

Article Title: Structure, mechanism, and inhibition of Hedgehog acyltransferase

doi: 10.1016/j.molcel.2021.11.018

Figure Lengend Snippet:

Article Snippet: 16:0 Ether Coenzyme A (palmityl-CoA, nhPalm-CoA) , Avanti Lipids , SKU 870740P.

Techniques: Virus, Recombinant, Modification, Expressing, Infection, Mutagenesis, Ligation, Protease Inhibitor, Plasmid Preparation, Staining, Synthesized, Purification, Transformation Assay, Proximity Ligation Assay, In Situ, Imaging, Software, Sterility

Journal: Journal of Biological Chemistry

Article Title: Scavenger Receptor BI-mediated Selective Uptake Is Required for the Remodeling of High Density Lipoprotein by Endothelial Lipase

doi: 10.1074/jbc.m807683200

Figure Lengend Snippet: FIGURE 1. EL modification of HDL increases SR-BI-mediated selective uptake in vitro. HDL was isolated from C57BL/6 control mice injected either withemptycontroladenovirusAdNullorwithadoseofAdhELthatresultedin an 50% decrease in plasma total cholesterol levels. LdlA cells lacking low densitylipoproteinreceptorsthatwerestablytransfectedwithamurineSR-BI cDNA were incubated with the respective double labeled HDL preparations (125I-tyramine cellobiose and 3H-cholesteryl ether), and selective uptake was assessed as described under “Experimental Procedures” (n 3). The data are presented as the means S.E. * indicates statistically significant differences compared with the respective AdNull-injected controls (p 0.05) as assessed by Mann-Whitney U test.

Article Snippet: HDL Kinetic Studies—Autologous HDL isolated by sequential ultracentrifugation (density, 1.063 d 1.21) was prepared from pooled mouse plasma and labeled with 125I-tyramine-cellobiose and cholesteryl hexadecyl ether (cholesteryl-1,2-3H; PerkinElmer Life Sciences) essentially as described (24), and 1 Ci of the 125I-tyramine-cellobiose-HDL and 1 million dpm of the 3H-CE-HDL were injected via the tail vein.

Techniques: Modification, In Vitro, Isolation, Control, Injection, Clinical Proteomics, Incubation, Labeling, MANN-WHITNEY

Journal: Journal of Biological Chemistry

Article Title: Scavenger Receptor BI-mediated Selective Uptake Is Required for the Remodeling of High Density Lipoprotein by Endothelial Lipase

doi: 10.1074/jbc.m807683200

Figure Lengend Snippet: FIGURE 3. HDL FCRs in C57BL/6 mice and SR-BI knock-out mice in response to injection of either the control adenovirus AdNull or the EL expressing adenovirus AdhEL. HDL kinetic experiments using autologous HDL double labeled with 125I-tyramine cellobiose and 3H-cholesteryl ether were carried out on day 5 following adenovirus injection as detailed under “Experimental Procedures” (n 6 mice/group). The data are presented as the means S.E. * indicates statistically significant differences compared with the respective AdNull-injected controls (at least p 0.05), and # indicates statistically significant differences (at least p 0.05) compared with the HDL protein (Prot.) FCR as assessed by Mann-Whitney U test.

Article Snippet: HDL Kinetic Studies—Autologous HDL isolated by sequential ultracentrifugation (density, 1.063 d 1.21) was prepared from pooled mouse plasma and labeled with 125I-tyramine-cellobiose and cholesteryl hexadecyl ether (cholesteryl-1,2-3H; PerkinElmer Life Sciences) essentially as described (24), and 1 Ci of the 125I-tyramine-cellobiose-HDL and 1 million dpm of the 3H-CE-HDL were injected via the tail vein.

Techniques: Knock-Out, Injection, Control, Expressing, Labeling, MANN-WHITNEY

Journal: Journal of Biological Chemistry

Article Title: Scavenger Receptor BI-mediated Selective Uptake Is Required for the Remodeling of High Density Lipoprotein by Endothelial Lipase

doi: 10.1074/jbc.m807683200

Figure Lengend Snippet: FIGURE 4. Catabolism of HDL apolipoproteins and HDL cholesteryl ester by liver and kidney in C57BL/6 mice and SR-BI knock-out mice in response to injection of either the control adenovirus AdNull or the EL expressing adenovirus AdhEL. The mice were administered labeled HDL on day 5 following adenovirusinjectionasdetailedunder“ExperimentalProcedures.”After24h,themiceweresacrificed,theorganswereharvested,anduptakeoftherespective tracers was assessed and expressed as a percentage of the initial injected dose. For calculation of selective uptake 125I-tyramine-cellobiose-labeled HDL uptake (% of injected dose) was subtracted from 3H-cholesteryl ether-labeled HDL uptake (% of injected dose) into the respective organs. A, liver HDL protein and cholesteryl ether uptake in wild-type (A) and SR-BI knockout (B) mice. C, liver selective uptake; D, kidney HDL protein and cholesteryl ether uptake in wild-type (D) and SR-BI knockout (E) mice; F, kidney selective uptake (n 6 mice/group). The data are presented as the means S.E. * indicates statistically significant differences compared with the respective AdNull-injected controls (at least p 0.05), and # indicates statistically significant differences (at least p 0.05) compared with the respective HDL protein (Prot.) uptake values C57BL/6 mice as assessed by Mann-Whitney U test.

Article Snippet: HDL Kinetic Studies—Autologous HDL isolated by sequential ultracentrifugation (density, 1.063 d 1.21) was prepared from pooled mouse plasma and labeled with 125I-tyramine-cellobiose and cholesteryl hexadecyl ether (cholesteryl-1,2-3H; PerkinElmer Life Sciences) essentially as described (24), and 1 Ci of the 125I-tyramine-cellobiose-HDL and 1 million dpm of the 3H-CE-HDL were injected via the tail vein.

Techniques: Knock-Out, Injection, Control, Expressing, Labeling, MANN-WHITNEY