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Image Search Results
Journal: bioRxiv
Article Title: Single cell transcriptome profiling of mouse and hESC-derived pancreatic progenitors
doi: 10.1101/289470
Figure Lengend Snippet: (A) Schematic overview of the two mouse lines used to isolate cell populations during pancreas development. Using this strategy, pancreatic progenitors (P; red) are tdTomato+, early Neurog3-lineage cells (N Early ; yellow) are tdTomato+ and eGFP+, and later Neurog3-lineage cells (N Late ; green) are eGFP+. (B) FACS plot of E15.5 cells used for library generation. (C) Within the E15.5 pancreatic cells there were 13 clusters of nine cell types: trunk, acinar, endocrine progenitor (EP), ductal, endocrine, macrophage (ϕ), neuron, vasculature, and mesenchyme. (D) Within the E18.5 pancreatic cells there were 14 clusters of 11 cell types: trunk, acinar, ductal, maturing endocrine cells (Endo), α-, β-, δ-cells, macrophage (ϕ), neuron, vasculature, and mesenchyme. (E-F) Single cell gene expression of tdTomato and eGFP at (E) E15.5 and (F) E18.5.
Article Snippet: Previously, a
Techniques: Expressing
Journal: bioRxiv
Article Title: Single cell transcriptome profiling of mouse and hESC-derived pancreatic progenitors
doi: 10.1101/289470
Figure Lengend Snippet: (A) Clustering of E15.5 yellow and green cells revealed seven clusters. These clusters were identified as endocrine progenitors (EP; 28.7%), Chga -expressing immature endocrine cells (C;16.7%), alpha-cells (α; 26.4%), β-cells (β; 18.4%), ghrelin-cells (ε; 3.9%), trunk cells (T; 4.7%), and macrophages (ϕ; 1.2%). (B) Single cell gene expression of Neurog3 , Ins1 , Ins2 , Gcg , and Ghrl across cell clusters. (C) Heat map of the top ten differentially expressed genes in EP (pink), α-cells (yellow), and β-cells (green) populations.
Article Snippet: Previously, a
Techniques: Expressing
Journal: bioRxiv
Article Title: Single cell transcriptome profiling of mouse and hESC-derived pancreatic progenitors
doi: 10.1101/289470
Figure Lengend Snippet: (A) Minimal spanning tree for E15.5 ductal (yellow), acinar (red), trunk (purple), endocrine progenitor (EP; blue), and endocrine cells (green). For this analysis, the top 1000 highly variant genes were determined using Seurat and cell states were ordered by Monocle. (B) Pseudotime from 0 (blue) to 30 (red) orders trunk cells first, following by ductal and then the endocrine lineage. (C) Gene expression of cell-type specific markers during pseudotime: acinar markers Cpa1 ; ductal markers Sox9 and Krt19 ; endocrine progenitor markers Neurog3 , Cdkn1a , Neurod1 , Mdk , and Gadd45a; pancreatic markers Nkx2-2, Nkx6-1 , Pdx1, Pax4 , and Pax6 ; and endocrine markers Chga , Ghrl , Mafb , Gcg , Ins1 , Ins2 , and Sst .
Article Snippet: Previously, a
Techniques: Variant Assay, Expressing
Journal: bioRxiv
Article Title: Single cell transcriptome profiling of mouse and hESC-derived pancreatic progenitors
doi: 10.1101/289470
Figure Lengend Snippet: (A) tSNE plot of 9 cell clusters from S6D1 GFP+. (B) Single cell gene expression of NEUROG3 , NEUROD1 , CDKN1A , GCG , INS , and SST . (C) Top ten differentially expressed genes in the endocrine progenitor (EP) cell clusters: EP1 (red), EP2 (green), and EP3 (blue). (D) Top ten differentially expressed genes in endocrine cell clusters: Endo1 (yellow), Endo2 (green), and Endo3 (blue).
Article Snippet: Previously, a
Techniques: Expressing