Journal: mBio

Article Title: Chlamydia trachomatis modulates the expression of JAK-STAT signaling components to attenuate the type II interferon response of epithelial cells

doi: 10.1128/mbio.01834-24

Figure Lengend Snippet: IFNγ-induced activation of STAT1 is attenuated in C. trachomatis L2-infected Hep2 cells. ( A ) Schematic representation of C. trachomatis serovar L2 infection and IFNγ treatment of epithelial HEp2 cells. ( B ) C. trachomatis -infected cells (multiplicity of infection [MOI] = 2, yellow outline for cell boundary as determined in bright-field channel), chlamydial inclusion visualized with human anti- C . trachomatis antibody in cyan) and uninfected HEp2 cells exposed to IFNγ (10 U/mL) starting at 24 hpi for 6 hours were fixed and stained for phosphorylated STAT1 (pSTAT1) in red and for DNA with DAPI in blue. ( C ) Nuclear pSTAT1 fluorescence intensities were quantified in both uninfected and infected cells. Measurements were normalized to respective no IFNγ control to account for background pSTAT1 staining and plotted as mean fluorescence. Violin plot represents six independent experiments with total of 260 nuclei measured per group. Statistical significance was determined by Wilcoxon rank sum. ( D ) Protein level of IFNγ-STAT1 downstream target IFO1 was assessed from total protein lysates collected from C. trachomatis -infected (MOI = 4) and uninfected cells exposed to IFNγ (10 U/mL) at 24 hpi for 6 hours. ( E ) Densitometric quantification of IDO1 plotted as ratios to the loading control α-tubulin. Bar graph represents four independent experiments, and statistical significance was determined using Welch’s t -test. * P < 0.05 and *** P < 0.001. Scale bar at 10 µm.

Article Snippet: HEp2 cells were seeded onto 12-mm acid-etched glass coverslips (Chemglass, CLS-1760-012) placed in 24-well plates.

Techniques: Activation Assay, Infection, Staining, Fluorescence, Control

Journal: mBio

Article Title: Chlamydia trachomatis modulates the expression of JAK-STAT signaling components to attenuate the type II interferon response of epithelial cells

doi: 10.1128/mbio.01834-24

Figure Lengend Snippet: C. trachomatis growth is inhibited in cells pretreated with IFNγ. Immunofluorescence visualization of C. trachomatis inclusions using the chlamydial protein cHsp60 in HEp2 cells following pre-treatment (Pre-12 + 24) with IFNγ for 12 hours before infection or co-treatment (Co-24) with IFNγ at time of infection. Scale bar at 20 µm.

Article Snippet: HEp2 cells were seeded onto 12-mm acid-etched glass coverslips (Chemglass, CLS-1760-012) placed in 24-well plates.

Techniques: Immunofluorescence, Infection

Journal: Science Advances

Article Title: A dominant function of LynB kinase in preventing autoimmunity

doi: 10.1126/sciadv.abj5227

Figure Lengend Snippet: Serum was collected from 8.5-month-old male and female mice and assayed for antibody production. ( A ) Serum ANA detection via FITC staining of Hep-2 cells. Occurrence of splenomegaly or low body mass in the same individual is indicated (+ yes, − no). ( B ) Frequency of ANA negativity (−) and positivity (+). Occurrence of splenomegaly or low body mass in the same individual (+ yes, − no) as defined in . Significance for raw contingency data was assessed via two-sided Fisher’s exact test with Bonferroni correction for multiple comparisons. Analysis pool: WT 4 M + 8 F, LynA KO 6 M + 6 F, LynB KO 4 M + 8 F, and Lyn KO 6 M + 6 F. ( C ) ELISA-derived IgM levels and associated splenomegaly or low body mass; error bars, SEM.

Article Snippet: Kallestad HEp-2 (Bio-Rad, catalog no. 30472) was used to detect ANA staining according to the manufacturer’s instruction, modified to detect mouse IgG.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: Nutrients

Article Title: Metabolic Health in Obese Subjects—Is There a Link to Lactoferrin and Lactoferrin Receptor-Related Gene Polymorphisms?

doi: 10.3390/nu12092843

Figure Lengend Snippet: Distribution of alleles and genotypes in analysed gene polymorphisms in MHO and MUHO subjects.

Article Snippet: SNPs in LTF rs1126477, LTF rs2239692, LTF rs1126478, low-density lipoprotein receptor-related protein 2 ( LRP2 ) rs2544390, LRP1 rs4759277, and LRP1 rs1799986, genes were genotyped with TaqMan allelic discrimination assays (Applied Biosystem, Foster City, NC, USA): rs2544390: C___8822318_10, rs4759277: C___31186847_10, rs1799986: C___1955081_10, rs1126477: C___9698511_10; rs2239692: C___2610649_10; rs1126478: C___9698521_10) using Bio-Rad CFX96TM Real-Time PCR system (Hercules, CA, USA).

Techniques:

Journal: Nutrients

Article Title: Metabolic Health in Obese Subjects—Is There a Link to Lactoferrin and Lactoferrin Receptor-Related Gene Polymorphisms?

doi: 10.3390/nu12092843

Figure Lengend Snippet: Odds ratios (OR) and 95% confidence interval (CI) for the associations between analysed gene polymorphisms and the prevalence of metabolic disorders in obese subjects.

Article Snippet: SNPs in LTF rs1126477, LTF rs2239692, LTF rs1126478, low-density lipoprotein receptor-related protein 2 ( LRP2 ) rs2544390, LRP1 rs4759277, and LRP1 rs1799986, genes were genotyped with TaqMan allelic discrimination assays (Applied Biosystem, Foster City, NC, USA): rs2544390: C___8822318_10, rs4759277: C___31186847_10, rs1799986: C___1955081_10, rs1126477: C___9698511_10; rs2239692: C___2610649_10; rs1126478: C___9698521_10) using Bio-Rad CFX96TM Real-Time PCR system (Hercules, CA, USA).

Techniques: