Journal: Experimental eye research

Article Title: Heterogeneity of Cultured Melanocyte Elongation and Proliferation Factor in Bilateral Diffuse Uveal Melanocytic Proliferation

doi: 10.1016/j.exer.2019.04.006

Figure Lengend Snippet: Description of human melanocyte growth media. Parentheses indicate final concentration of added plasma fractions.

Article Snippet: Plasmapheresis samples were collected from the 1 st session, the final session, and frozen at −80°C. (4.2) Cell Culture Experiments Primary human melanocyte cells (HEMnLP, Cat #C0025C) were purchased from Thermo Fisher Scientific (Waltham, MA).

Techniques: Concentration Assay, Clinical Proteomics, Control

Journal: Experimental eye research

Article Title: Heterogeneity of Cultured Melanocyte Elongation and Proliferation Factor in Bilateral Diffuse Uveal Melanocytic Proliferation

doi: 10.1016/j.exer.2019.04.006

Figure Lengend Snippet: BDUMP plasma increased melanocyte growth. Melanocytes were cultured for 3 days (A), 4 days (B), and 5 days (C) in the presence of 1% HMGS growth medium, 0.5% HMGS control medium, and 0.5% BDUMP medium with 0.5% HMGS. Data were normalized to control medium for each day. BDUMP medium increased melanocyte growth at Day 3, Day 4, and Day 5 (N = 5). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Plasmapheresis samples were collected from the 1 st session, the final session, and frozen at −80°C. (4.2) Cell Culture Experiments Primary human melanocyte cells (HEMnLP, Cat #C0025C) were purchased from Thermo Fisher Scientific (Waltham, MA).

Techniques: Clinical Proteomics, Cell Culture, Control

Journal: Experimental eye research

Article Title: Heterogeneity of Cultured Melanocyte Elongation and Proliferation Factor in Bilateral Diffuse Uveal Melanocytic Proliferation

doi: 10.1016/j.exer.2019.04.006

Figure Lengend Snippet: CMEP factor was not enriched in the IgG fraction. Electrophoresis (A) of unfractionated plasma, flow through (FT), and elution showed increased abundance of the IgG heavy chain (IgG HC) 50 kDa band and the IgG light chain (IgG LC) 25 kDa band in the elution and decreased abundance of these bands in the flow through. Immunoblot analysis for IgG heavy chain (B) demonstrated large amounts of IgG heavy chain in the elution and unfractionated plasma with barely detectable IgG heavy chain in the flow through. (C) Melanocytes were cultured for 4 days in the presence of control 0.5% HMGS medium, positive control unfractionated BDUMP plasma medium, IgG depleted [IgG (−)] medium, and IgG enriched [IgG (+)] medium. Unfractionated BDUMP medium stimulated the most melanocyte growth. IgG depleted and enriched medium mildly increased melanocyte growth (N=5). * p < 0.05, ** p < 0.01, *** p < 0.001 vs control 0.5%. # p < 0.05, ## p < 0.01 vs unfractionated BDUMP.

Article Snippet: Plasmapheresis samples were collected from the 1 st session, the final session, and frozen at −80°C. (4.2) Cell Culture Experiments Primary human melanocyte cells (HEMnLP, Cat #C0025C) were purchased from Thermo Fisher Scientific (Waltham, MA).

Techniques: Electrophoresis, Clinical Proteomics, Western Blot, Cell Culture, Control, Positive Control

Journal: Experimental eye research

Article Title: Heterogeneity of Cultured Melanocyte Elongation and Proliferation Factor in Bilateral Diffuse Uveal Melanocytic Proliferation

doi: 10.1016/j.exer.2019.04.006

Figure Lengend Snippet: BDUMP plasma after plasmapheresis stimulates melanocyte growth. Electrophersis (A) of pre- and post-plasmapheresis BDUMP plasma showed less IgG heavy and light chain after 6 plasmapheresis sessions. (B) Melanocytes were cultured for 4 days in the presence of control 0.5% HMGS medium, pre-plasmapheresis BDUMP medium, and post-plasmapheresis BDUMP medium. Both pre- and post-plasmaphersis BDUMP medium stimulated equal melanocyte growth (N=3, * p < 0.05 vs control).

Article Snippet: Plasmapheresis samples were collected from the 1 st session, the final session, and frozen at −80°C. (4.2) Cell Culture Experiments Primary human melanocyte cells (HEMnLP, Cat #C0025C) were purchased from Thermo Fisher Scientific (Waltham, MA).

Techniques: Clinical Proteomics, Cell Culture, Control