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Image Search Results
Journal: Nature communications
Article Title: CEP63 deficiency promotes p53-dependent microcephaly and reveals a role for the centrosome in meiotic recombination
doi: 10.1038/ncomms8676
Figure Lengend Snippet: ( a ) Comparison of testicle weight to WT littermates at p10, p60 and p165. Examples of dissected p60 testes are shown (n = 4, 3, 8, 8, 2 and 4 animals, respectively). ( b ) Testes sections from p5 animals were stained with antibody against WT1 (upper panels) and WT1 negative cells (spermatagonia, red arrows) were scored and plotted (lower panels). Reduced cell numbers in p5 tubules (bottom left panel) but proportionally similar numbers of spermatagonia (bottom right panel) were observed (results are combined from 4 Cep63 +/T and 3 Cep63 T/T animals. For Cep63 +/T n = 201 and Cep63 T/T n = 143 tubules scored). Individual values are shown in a scatterplot with the average (red line) and standard deviation (black lines) indicated. Tetraploidy was observed in some spermatagonia from Cep63 T/T animals (upper right panel). ( c ) H&E staining of testes sections from p60 mice showing relative size and condition of tubules (scale bar = 100 µm in 10X panels and 50 µm in 40X panels). ( d ) Quantification of tubule size in the indicated genotypes (2 animals per genotype, n = 6 tubules per genotype measured). ( e ) Examples of TUNEL staining (scale bar = 50 µm). ( f ) Quantification of TUNEL positive tubules (n = 3 animals per genotype used, 279 Cep63 +/+ and 266 Cep63 T/T tubules scored). ( g ) Examples of aberrant spermatids from Cep63 T/T testes squashes. DAPI stained tails are indicated with yellow arrows (scale bar = 5 µm). ( h ) Sperm counts from mice of the indicated genotypes (n = 4, 2 and 4 animals per genotype, respectively). All graphs with error bars are presented as the average plus standard deviation. Asterisks denote statistical significance (n.s.= not significant, * = p-value <0.05, ** = p-value < 0.01, *** = p-value < 0.001) determined by the unpaired two-way Student’s T-test (4a, 4d, 4f) or the unpaired two-way Wilcoxon rank sum test (4b).
Article Snippet: 5 days old testes were immuno-labeled with
Techniques: Comparison, Staining, Standard Deviation, TUNEL Assay