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    TaKaRa hela tet off cells
    Observation of mitophagy using mito-Keima cells. ( A ) <t>Tet-off</t> mito-Keima-expressing <t>HeLa</t> cells (mito-Keima cells) were cultured in complete medium or Krebs-Henseleit buffer (starvation) with or without 10 mM 3-MA for 18 h. Mito-Keima localized within the mitochondria was excited by 440-nm light (Keima [mitochondria]; red) and mito-Keima delivered within lysosomes by mitophagy was excited by 590-nm light (Keima [autolysosomes]; green) (emission, 624 nm). Bar = 10 μm. The number of acidic puncta per cell was counted. Data are expressed as means ± SD of at least 30 cells from 3 separate experiments. ( B ) Mito-Keima cells were transfected with control siRNA or PIK3C3 -specific siRNA, and then cultured in complete medium or Krebs-Henseleit buffer for 18 h. Bar = 10 μm. The number of acidic puncta per cell was counted. Data are expressed as means ± SD of at least 30 cells from 3 separate experiments.
    Hela Tet Off Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    93
    TaKaRa hela tet on cells
    Observation of mitophagy using mito-Keima cells. ( A ) <t>Tet-off</t> mito-Keima-expressing <t>HeLa</t> cells (mito-Keima cells) were cultured in complete medium or Krebs-Henseleit buffer (starvation) with or without 10 mM 3-MA for 18 h. Mito-Keima localized within the mitochondria was excited by 440-nm light (Keima [mitochondria]; red) and mito-Keima delivered within lysosomes by mitophagy was excited by 590-nm light (Keima [autolysosomes]; green) (emission, 624 nm). Bar = 10 μm. The number of acidic puncta per cell was counted. Data are expressed as means ± SD of at least 30 cells from 3 separate experiments. ( B ) Mito-Keima cells were transfected with control siRNA or PIK3C3 -specific siRNA, and then cultured in complete medium or Krebs-Henseleit buffer for 18 h. Bar = 10 μm. The number of acidic puncta per cell was counted. Data are expressed as means ± SD of at least 30 cells from 3 separate experiments.
    Hela Tet On Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hela tet on cells/product/TaKaRa
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hela tet on cells - by Bioz Stars, 2022-10
    93/100 stars
      Buy from Supplier

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    Observation of mitophagy using mito-Keima cells. ( A ) Tet-off mito-Keima-expressing HeLa cells (mito-Keima cells) were cultured in complete medium or Krebs-Henseleit buffer (starvation) with or without 10 mM 3-MA for 18 h. Mito-Keima localized within the mitochondria was excited by 440-nm light (Keima [mitochondria]; red) and mito-Keima delivered within lysosomes by mitophagy was excited by 590-nm light (Keima [autolysosomes]; green) (emission, 624 nm). Bar = 10 μm. The number of acidic puncta per cell was counted. Data are expressed as means ± SD of at least 30 cells from 3 separate experiments. ( B ) Mito-Keima cells were transfected with control siRNA or PIK3C3 -specific siRNA, and then cultured in complete medium or Krebs-Henseleit buffer for 18 h. Bar = 10 μm. The number of acidic puncta per cell was counted. Data are expressed as means ± SD of at least 30 cells from 3 separate experiments.

    Journal: Autophagy

    Article Title: Mitophagy is primarily due to alternative autophagy and requires the MAPK1 and MAPK14 signaling pathways

    doi: 10.1080/15548627.2015.1023047

    Figure Lengend Snippet: Observation of mitophagy using mito-Keima cells. ( A ) Tet-off mito-Keima-expressing HeLa cells (mito-Keima cells) were cultured in complete medium or Krebs-Henseleit buffer (starvation) with or without 10 mM 3-MA for 18 h. Mito-Keima localized within the mitochondria was excited by 440-nm light (Keima [mitochondria]; red) and mito-Keima delivered within lysosomes by mitophagy was excited by 590-nm light (Keima [autolysosomes]; green) (emission, 624 nm). Bar = 10 μm. The number of acidic puncta per cell was counted. Data are expressed as means ± SD of at least 30 cells from 3 separate experiments. ( B ) Mito-Keima cells were transfected with control siRNA or PIK3C3 -specific siRNA, and then cultured in complete medium or Krebs-Henseleit buffer for 18 h. Bar = 10 μm. The number of acidic puncta per cell was counted. Data are expressed as means ± SD of at least 30 cells from 3 separate experiments.

    Article Snippet: The HeLa Tet-off cell line was obtained from Clontech (631156).

    Techniques: Expressing, Cell Culture, Transfection