Journal: iScience

Article Title: Heat-induced SIRT1-mediated H4K16ac deacetylation impairs resection and SMARCAD1 recruitment to double strand breaks

doi: 10.1016/j.isci.2022.104142

Figure Lengend Snippet:

Article Snippet: Hela human cell line , ATCC , CRL-12401; RRID: CVCL_W341.

Techniques: Virus, Generated, Control, Recombinant, Protease Inhibitor, Extraction, cDNA Synthesis, Plasmid Preparation, Software

Journal: Science Advances

Article Title: Human DNA topoisomerase I poisoning causes R loop–mediated genome instability attenuated by transcription factor IIS

doi: 10.1126/sciadv.adm8196

Figure Lengend Snippet: ( A and B ) Enrichment analysis of observed R loop gain with and without (w/o) DSBs at early, early/mid, and late IZs (A) and at early, early/mid, and late IZs and in TAD classes (B) assessed as in . ( C ) PLA assay of PCNA with RNAPII in cells expressing (+ doxy) or not (− doxy) TFIISm. CTRL, control samples using the indicated antibody only. Fold changes of CPT-treated versus untreated and induced versus uninduced samples are reported over each bar. Mean of analyzed cells is 800. Statistical significance was calculated with one-tailed paired t test. ( D ) Experimental protocol of 1-hour CPT treatment with single (top) or dual (bottom) labeling of HeLa cells. ( E ) Representative images of the possible cases of dual labeled cells. ( F to H ) Levels of micronuclei triggered in late G 1 , late S, or early/mid S phase [as in (E)], respectively. Micronucleus levels are reported as micronuclei (MNi) per 100 cells (left) or as fold change in RNase H1–expressing (RH) versus control cells (right). Means of analyzed cells are 250 (F), 150 (G), and 400 (H). Statistical significance was calculated comparing micronucleus distribution of treated over untreated samples with two-tailed Mann-Whitney test. ( I and J ) γH2AX detected by immunofluorescence microscopy at the end of EdU administration (I) or at the end of BrdU administration (J) as in (D). Levels of nuclear fluorescence are reported as fold change over untreated cells (number of analyzed cells is 450). Statistical significance was calculated considering treated over untreated samples with one-tailed ratio paired t test. In (C) and (F) to (J), bars show means ± SEM of three biological replicates. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Human cancer HCT116 cells, U2OS, and HeLa cell lines were purchased from American Type Culture Collection (LGC Standards S.r.l., Milan, Italy).

Techniques: Expressing, Control, One-tailed Test, Labeling, Two Tailed Test, MANN-WHITNEY, Immunofluorescence, Microscopy, Fluorescence

Journal: Infection and Immunity

Article Title: Regulators Encoded in the Escherichia coli Type III Secretion System 2 Gene Cluster Influence Expression of Genes within the Locus for Enterocyte Effacement in Enterohemorrhagic E. coli O157:H7

doi: 10.1128/iai.72.12.7282-7293.2004

Figure Lengend Snippet: FIG. 6. Adhesion to human epithelial cells. (A to C) Int407 cells infected with the parent strain (A), etrA mutant LZ2 (B), or eivF mutant LZ3 (C) and subjected to Hemacolor staining. Magnification, 400. (D) Semiquantitative analysis of adherence. The number of microcolonies (a microcolony consisted of 10 adherent bacteria) per cell was calculated from 20 fields for each experiment, and the means were determined from three independent experiments. An average of 140 cells were examined in each field.

Article Snippet: Adhesion of E. coli strains to Int407 cells (human embryonic intestine; ATCC CCL 6) was quantified essentially as described previously (38).

Techniques: Infection, Mutagenesis, Staining, Bacteria

Journal: mBio

Article Title: Unconventional N-Linked Glycosylation Promotes Trimeric Autotransporter Function in Kingella kingae and Aggregatibacter aphrophilus

doi: 10.1128/mBio.01206-15

Figure Lengend Snippet: EmaA is an adhesin and involved in autoaggregation. (A) Mean adherence to human epithelial cells by A. aphrophilus with the wild-type emaA gene, an interrupted emaA gene, and the interrupted emaA gene reverted to the wild type. (B) Mean autoaggregation of A. aphrophilus with the wild-type emaA gene, an interrupted emaA gene, and the interrupted emaA gene reverted to the wild type. Each graph shows data from biological replicates performed in triplicate. Error bars represent standard errors. An asterisk denotes a P value of <0.05 obtained from a paired t test.

Article Snippet: Quantitative adherence assays were performed as previously described using Chang epithelial cells (ATCC CCL 20.2) ( ).

Techniques: