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    TaKaRa hek293 cells
    Prolonged time course for the secretion of CEL. CEL-MUT protein appears to be more stable than the CEL-WT. <t>HEK293</t> cells were metabolically labeled and chased for the indicated time periods, and the proteins in cell extract and growth medium were immunoprecipitated
    Hek293 Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek293 cells/product/TaKaRa
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hek293 cells - by Bioz Stars, 2022-11
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    93
    TaKaRa hek293 tet on cells
    Establishment of <t>HEK293</t> <t>TET-on</t> HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.
    Hek293 Tet On Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek293 tet on cells/product/TaKaRa
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hek293 tet on cells - by Bioz Stars, 2022-11
    93/100 stars
      Buy from Supplier

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    Prolonged time course for the secretion of CEL. CEL-MUT protein appears to be more stable than the CEL-WT. HEK293 cells were metabolically labeled and chased for the indicated time periods, and the proteins in cell extract and growth medium were immunoprecipitated

    Journal: The Journal of Biological Chemistry

    Article Title: Diabetes and Pancreatic Exocrine Dysfunction Due to Mutations in the Carboxyl Ester Lipase Gene-Maturity Onset Diabetes of the Young (CEL-MODY): A PROTEIN MISFOLDING DISEASE*

    doi: 10.1074/jbc.M111.222679

    Figure Lengend Snippet: Prolonged time course for the secretion of CEL. CEL-MUT protein appears to be more stable than the CEL-WT. HEK293 cells were metabolically labeled and chased for the indicated time periods, and the proteins in cell extract and growth medium were immunoprecipitated

    Article Snippet: Similarly, the highly positively charged TR domain of CEL-MUT would favor its interaction with membranes (phospholipid headgroups) as actually observed as aggregates at the plasma membrane of HEK293 cells in this study ( A ).

    Techniques: Metabolic Labelling, Labeling, Immunoprecipitation

    UPR in HEK293 cells expressing different CEL proteins. Detergent-soluble proteins in lysates of cells expressing CEL-WT, CEL-MUT, and EV were analyzed by immunoblotting using specific antibodies (see under “Experimental Procedures”). A

    Journal: The Journal of Biological Chemistry

    Article Title: Diabetes and Pancreatic Exocrine Dysfunction Due to Mutations in the Carboxyl Ester Lipase Gene-Maturity Onset Diabetes of the Young (CEL-MODY): A PROTEIN MISFOLDING DISEASE*

    doi: 10.1074/jbc.M111.222679

    Figure Lengend Snippet: UPR in HEK293 cells expressing different CEL proteins. Detergent-soluble proteins in lysates of cells expressing CEL-WT, CEL-MUT, and EV were analyzed by immunoblotting using specific antibodies (see under “Experimental Procedures”). A

    Article Snippet: Similarly, the highly positively charged TR domain of CEL-MUT would favor its interaction with membranes (phospholipid headgroups) as actually observed as aggregates at the plasma membrane of HEK293 cells in this study ( A ).

    Techniques: Expressing

    CEL-MUT protein forms higher oligomeric forms that are SDS-resistant and Triton X-100-insoluble. A , metabolically labeling of HEK293 cells, stably expressing CEL-WT ( left panel ) and CEL-MUT ( right panel ). Subsequently, the cells were lysed, and the CEL

    Journal: The Journal of Biological Chemistry

    Article Title: Diabetes and Pancreatic Exocrine Dysfunction Due to Mutations in the Carboxyl Ester Lipase Gene-Maturity Onset Diabetes of the Young (CEL-MODY): A PROTEIN MISFOLDING DISEASE*

    doi: 10.1074/jbc.M111.222679

    Figure Lengend Snippet: CEL-MUT protein forms higher oligomeric forms that are SDS-resistant and Triton X-100-insoluble. A , metabolically labeling of HEK293 cells, stably expressing CEL-WT ( left panel ) and CEL-MUT ( right panel ). Subsequently, the cells were lysed, and the CEL

    Article Snippet: Similarly, the highly positively charged TR domain of CEL-MUT would favor its interaction with membranes (phospholipid headgroups) as actually observed as aggregates at the plasma membrane of HEK293 cells in this study ( A ).

    Techniques: Metabolic Labelling, Labeling, Stable Transfection, Expressing

    CEL-MUT forms extra- and intracellular aggregates in HEK293 cells. HEK293 cells stably transfected with CEL-MUT and CEL-WT were processed for immunoperoxidase electron microscopy as described under “Experimental Procedures.” A , extracellular

    Journal: The Journal of Biological Chemistry

    Article Title: Diabetes and Pancreatic Exocrine Dysfunction Due to Mutations in the Carboxyl Ester Lipase Gene-Maturity Onset Diabetes of the Young (CEL-MODY): A PROTEIN MISFOLDING DISEASE*

    doi: 10.1074/jbc.M111.222679

    Figure Lengend Snippet: CEL-MUT forms extra- and intracellular aggregates in HEK293 cells. HEK293 cells stably transfected with CEL-MUT and CEL-WT were processed for immunoperoxidase electron microscopy as described under “Experimental Procedures.” A , extracellular

    Article Snippet: Similarly, the highly positively charged TR domain of CEL-MUT would favor its interaction with membranes (phospholipid headgroups) as actually observed as aggregates at the plasma membrane of HEK293 cells in this study ( A ).

    Techniques: Stable Transfection, Transfection, Electron Microscopy

    In vitro N - and O -linked deglycosylation of CEL proteins variants. Acetone-precipitated proteins from the cell-free growth medium of HEK293 cells, stably expressing CEL-WT and CEL-MUT, were subjected to N -linked ( N -glycosidase F) and/or O -linked ( O -glycosidase

    Journal: The Journal of Biological Chemistry

    Article Title: Diabetes and Pancreatic Exocrine Dysfunction Due to Mutations in the Carboxyl Ester Lipase Gene-Maturity Onset Diabetes of the Young (CEL-MODY): A PROTEIN MISFOLDING DISEASE*

    doi: 10.1074/jbc.M111.222679

    Figure Lengend Snippet: In vitro N - and O -linked deglycosylation of CEL proteins variants. Acetone-precipitated proteins from the cell-free growth medium of HEK293 cells, stably expressing CEL-WT and CEL-MUT, were subjected to N -linked ( N -glycosidase F) and/or O -linked ( O -glycosidase

    Article Snippet: Similarly, the highly positively charged TR domain of CEL-MUT would favor its interaction with membranes (phospholipid headgroups) as actually observed as aggregates at the plasma membrane of HEK293 cells in this study ( A ).

    Techniques: In Vitro, Stable Transfection, Expressing

    Time course for the secretion of CEL-WT and CEL-MUT by stably transfected HEK293 cells. A , pulse-chase analysis of HEK293 stably expressing CEL-WT ( left panel ) and CEL-MUT ( right panel ). The cells were metabolically labeled with [ 35 S]Met/Cys for 5 min,

    Journal: The Journal of Biological Chemistry

    Article Title: Diabetes and Pancreatic Exocrine Dysfunction Due to Mutations in the Carboxyl Ester Lipase Gene-Maturity Onset Diabetes of the Young (CEL-MODY): A PROTEIN MISFOLDING DISEASE*

    doi: 10.1074/jbc.M111.222679

    Figure Lengend Snippet: Time course for the secretion of CEL-WT and CEL-MUT by stably transfected HEK293 cells. A , pulse-chase analysis of HEK293 stably expressing CEL-WT ( left panel ) and CEL-MUT ( right panel ). The cells were metabolically labeled with [ 35 S]Met/Cys for 5 min,

    Article Snippet: Similarly, the highly positively charged TR domain of CEL-MUT would favor its interaction with membranes (phospholipid headgroups) as actually observed as aggregates at the plasma membrane of HEK293 cells in this study ( A ).

    Techniques: Stable Transfection, Transfection, Pulse Chase, Expressing, Metabolic Labelling, Labeling

    Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.

    Journal: Molecular and Cellular Biology

    Article Title: Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation

    doi: 10.1128/MCB.23.24.9361-9374.2003

    Figure Lengend Snippet: Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.

    Article Snippet: HIF-1αDPA and HIF-2αDPA cDNAs under transcriptional control of the TRE were stably transfected into HEK293 TET-on cells, which express the reverse TET-controlled transactivator.

    Techniques: Expressing, Northern Blot, Concentration Assay