Journal: Life

Article Title: Casomorphine-10 (CM-10) Peptide Orchestrates Circadian and Neurodevelopmental Gene Clusters via δ-Opioid Receptor Signaling: Insights from Transcriptome Analysis with δ-Opioid Receptor-Expressing HEK293 Cells

doi: 10.3390/life15101636

Figure Lengend Snippet: δ-opioid receptor (DOR)-expressing HEK293 cells and HEK293 cells stained with anti-DOR antibody and followed with FITC-conjugated anti-mouse IgG ( A ). Volcano plot of identified differentially expressed genes (DEGs) in DOR-HEK293 cells after 1 h treatment with CM-10 ( B ). Red boxes show changed gene expression over 1.4-time higher and less than 0.71 with p -value predicted by EdgeR of less than 0.05.

Article Snippet: The fixed DOR-HEK293 and HEK293 cells were then incubated with a rabbit anti-DOR antibody (GeneTex, Irvine, CA, USA) after dilution with 1% casein in PBS (1/1000) and stained with a secondary antibody (Cy3-conjugated anti-rabbit IgG, Rockland Inc., PA, USA).

Techniques: Expressing, Staining, Gene Expression

Journal: Life

Article Title: Casomorphine-10 (CM-10) Peptide Orchestrates Circadian and Neurodevelopmental Gene Clusters via δ-Opioid Receptor Signaling: Insights from Transcriptome Analysis with δ-Opioid Receptor-Expressing HEK293 Cells

doi: 10.3390/life15101636

Figure Lengend Snippet: Predicted DOR agonistic signaling networks in DOR-HEK293 cells after 1 h treatment with CM-10. Fourteen genes with suggested involvement in cAMP-dependent protein kinases, transcriptional regulators in response to cAMP, circadian rhythm, stress and depression are shown in and were applied for network analysis by STRING. Red: circadian rhythm, Green: regulation of transcription of Notch receptor target, Yellow: PKA activation in glucagon signalling.

Article Snippet: The fixed DOR-HEK293 and HEK293 cells were then incubated with a rabbit anti-DOR antibody (GeneTex, Irvine, CA, USA) after dilution with 1% casein in PBS (1/1000) and stained with a secondary antibody (Cy3-conjugated anti-rabbit IgG, Rockland Inc., PA, USA).

Techniques: Activation Assay

Journal: Molecules

Article Title: Sacha Inchi ( Plukenetia volubilis L.) Oil Press-Cake Powder: Chemical Characterization and In Vitro Bioactivity for Sustainable Applications

doi: 10.3390/molecules31010117

Figure Lengend Snippet: Effect of SICP Extract on Cell Viability in Different Cell Lines. Cell viability was assessed using the MTT assay following 72 h of incubation with SICP extract (SIPC, dilution 1:80) in different human cell lines: ( A ) HEK (human embryonic kidney, non-tumoral control), ( B ) Panc-1 (pancreatic ductal adenocarcinoma), ( C ) HepG2 (hepatocellular carcinoma), and ( D ) PC-3 (prostate adenocarcinoma). NT indicates untreated control cells; S1 indicates cells treated with the solvent mixture alone (acetone/water/acetic acid, 80/19/1 v / v / v ), and SIPC indicates cells treated with SICP extracts. Results are expressed as percentage of cell viability relative to untreated controls (NT, 100% viability). Each bar represents the mean ± standard error of the mean (SEM), and the number inside each bar indicates the number of independent biological replicates performed. Statistical significance was evaluated by one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05 vs. NT control).

Article Snippet: Panc-1 (human pancreatic ductal adenocarcinoma), HepG2 (human hepatocellular carcinoma), Calu-3 (human lung adenocarcinoma), PC-3 (human prostate adenocarcinoma), and HEK (human embryonic kidney) cell lines were maintained in appropriate growth media following ATCC recommendations.

Techniques: MTT Assay, Incubation, Control, Solvent