Journal: bioRxiv

Article Title: Oncostatin M orchestrates collective epithelial migration via HIF1A activation

doi: 10.1101/2025.09.26.678830

Figure Lengend Snippet: (A) MCF10A cells were treated with EGF, OSM, or IFNG for 48 hours, then stained for DAPI (blue) and β-Catenin (red). (B) Cell velocity vectors represent speed and direction of cell motility, derived from cell tracking data during 24-48 hours after ligand treatment. Arrow size is proportional to velocity. (C-F) Quantification of cell phenotype from live-cell imaging. Shaded region represents 95% confidence interval from 3 biological replicates (n=3). Boxplots in (E) depict interquartile range of nearest neighbor distance calculated for each cell. (G-J) Line plots show protein expression levels normalized to the T0 control for each treatment condition. Error bars represent the full range of measurements (n=3).

Article Snippet: Afterward, cells were treated with either 10 ng/ml EGF (R&D Systems #236-EG), 10 ng/ml OSM (R&D Systems #8475-OM), or 20 ng/ml IFNG (R&D Systems #258-IF) + 10 ng/mL EGF.

Techniques: Staining, Derivative Assay, Cell Tracking Assay, Live Cell Imaging, Expressing, Control

Journal: bioRxiv

Article Title: Oncostatin M orchestrates collective epithelial migration via HIF1A activation

doi: 10.1101/2025.09.26.678830

Figure Lengend Snippet: (A) Workflow for the comparative network analysis to identify molecular subnetworks and nodes perturbed by OSM. Integrated molecular data collected from OSM and IFNG treated cells was analyzed using CausalPath. (B) Combined molecular network of OSM and IFNG activated nodes. The rewiring score between conditions is indicated by node color, and the top scoring nodes nominated for further investigation are indicated by increased size. (C) The top scoring nodes most rewired by OSM treatment, corresponding to the highlighted nodes in (B). (D) The majority (8/14) of top rewired nodes are centered around the STAT3 subnetwork.

Article Snippet: Afterward, cells were treated with either 10 ng/ml EGF (R&D Systems #236-EG), 10 ng/ml OSM (R&D Systems #8475-OM), or 20 ng/ml IFNG (R&D Systems #258-IF) + 10 ng/mL EGF.

Techniques:

Journal: Life

Article Title: Casomorphine-10 (CM-10) Peptide Orchestrates Circadian and Neurodevelopmental Gene Clusters via δ-Opioid Receptor Signaling: Insights from Transcriptome Analysis with δ-Opioid Receptor-Expressing HEK293 Cells

doi: 10.3390/life15101636

Figure Lengend Snippet: δ-opioid receptor (DOR)-expressing HEK293 cells and HEK293 cells stained with anti-DOR antibody and followed with FITC-conjugated anti-mouse IgG ( A ). Volcano plot of identified differentially expressed genes (DEGs) in DOR-HEK293 cells after 1 h treatment with CM-10 ( B ). Red boxes show changed gene expression over 1.4-time higher and less than 0.71 with p -value predicted by EdgeR of less than 0.05.

Article Snippet: The fixed DOR-HEK293 and HEK293 cells were then incubated with a rabbit anti-DOR antibody (GeneTex, Irvine, CA, USA) after dilution with 1% casein in PBS (1/1000) and stained with a secondary antibody (Cy3-conjugated anti-rabbit IgG, Rockland Inc., PA, USA).

Techniques: Expressing, Staining, Gene Expression

Journal: Life

Article Title: Casomorphine-10 (CM-10) Peptide Orchestrates Circadian and Neurodevelopmental Gene Clusters via δ-Opioid Receptor Signaling: Insights from Transcriptome Analysis with δ-Opioid Receptor-Expressing HEK293 Cells

doi: 10.3390/life15101636

Figure Lengend Snippet: Predicted DOR agonistic signaling networks in DOR-HEK293 cells after 1 h treatment with CM-10. Fourteen genes with suggested involvement in cAMP-dependent protein kinases, transcriptional regulators in response to cAMP, circadian rhythm, stress and depression are shown in and were applied for network analysis by STRING. Red: circadian rhythm, Green: regulation of transcription of Notch receptor target, Yellow: PKA activation in glucagon signalling.

Article Snippet: The fixed DOR-HEK293 and HEK293 cells were then incubated with a rabbit anti-DOR antibody (GeneTex, Irvine, CA, USA) after dilution with 1% casein in PBS (1/1000) and stained with a secondary antibody (Cy3-conjugated anti-rabbit IgG, Rockland Inc., PA, USA).

Techniques: Activation Assay

Journal: Molecular Therapy Advances

Article Title: Manufacture of adeno-associated virus vectors by a novel human-derived cell line HAT and comprehensive evaluation of the vectors

doi: 10.1016/j.omta.2026.201700

Figure Lengend Snippet: Design space analysis for optimization of rAAV transcription conditions (A–D) Design space (DS) plots showing the probabilities of the rAAV titer and EF ratio of HAT-cell-produced AAV2 (A), AAV5 (B), and AAV9 (C) and HEK293-cell-produced AAV9 (D) in associated parameter ranges. Left, center, and right images show when the ratios of pGOI to pHelper, pGOI to pRepCap, and PEI-pro to DNA were kept at the DS-optimized values, respectively. The red and blue spaces and dots indicate the ranges corresponding to high predicted values of titer and EF ratio, respectively. The selected optimal setpoints are marked with black circles.

Article Snippet: For the resDNASEQ Quantitative HEK293 DNA Kit, the standard curve was generated using Quantitative HEK293 Genomic DNA (1592106, American Type Culture Collection).

Techniques: Produced

Journal: Molecular Therapy Advances

Article Title: Manufacture of adeno-associated virus vectors by a novel human-derived cell line HAT and comprehensive evaluation of the vectors

doi: 10.1016/j.omta.2026.201700

Figure Lengend Snippet: Comparison of rAAV productivity between the HAT and HEK293 cells (A and B) Bar graphs showing the results for HAT cell products in the design-of-experiment (DoE)-optimized conditions and HEK293 cell products in the typical transfection conditions as the genome titer (A) and the EF ratios (B). The genome titers were determined from clarified cell lysates, while the EF ratios were determined from small-scale affinity-purified material. Bars indicating HAT- and HEK293-produced AAV2 are green and light green, respectively; bars indicating HAT- and HEK293-produced AAV5 are red and light red, respectively; bars indicating HAT- and HEK293-produced AAV9 are cyan and orange, respectively. In the flask batches, error bars are ±SD of the mean of triplicate batches per condition. ∗∗ p < 0.01. The dots show values for each batch. (C and D) Bar graphs showing the results for HAT and HEK293 cell products in the DoE-optimized conditions as the genome titer (C) and the EF ratio (D). Error bars are ±SD of the mean of triplicate batches per condition. ∗∗ p < 0.01. ND, not detectable due to insufficient capsid titer for MP analysis. (E) Profiles of virus genome titer and capsid titer from HAT cells (genome titer, cyan line and cyan circle plot; capsid titer, blue line and blue circle plot) and HEK293 cells (genome titer, orange line and orange circle plot; capsid titer, deep-red line and deep-red circle plot) over 72 h after transfection in each DoE-optimized condition. Error bars are ±SD of the mean of triplicate batches. ∗ p < 0.05 and ∗∗ p < 0.01 vs. HEK293 cells. (F) Profiles of cell-specific virus genome titer and cell-specific capsid titer of HAT cells and HEK293 cells over 72 h after transfection in each DoE-optimized condition. Lines and plots use the same color-coding as in (E). Error bars are ±SD of the mean of triplicate batches. ∗ p < 0.05 vs. HEK293 cells.

Article Snippet: For the resDNASEQ Quantitative HEK293 DNA Kit, the standard curve was generated using Quantitative HEK293 Genomic DNA (1592106, American Type Culture Collection).

Techniques: Comparison, Transfection, Affinity Purification, Produced, Virus

Journal: Molecular Therapy Advances

Article Title: Manufacture of adeno-associated virus vectors by a novel human-derived cell line HAT and comprehensive evaluation of the vectors

doi: 10.1016/j.omta.2026.201700

Figure Lengend Snippet: Comparison of QAs between the HAT- and HEK293-cell-derived products (A) Analytical methods used for rAAV characterization in this study. (B) Representative cryo-electron micrographs of the purified AAV2, AAV5, and AAV9 produced using HAT and HEK293 cells. Scale bars, 50 nm. (C–E) Bar graphs showing the analytical results for the purified products derived from HAT and HEK293 cells: EF ratio (C), viral protein (VP) purity (D), and (VP1 + VP2)/60 VP values (E). Bars use the same color-coding as in .

Article Snippet: For the resDNASEQ Quantitative HEK293 DNA Kit, the standard curve was generated using Quantitative HEK293 Genomic DNA (1592106, American Type Culture Collection).

Techniques: Comparison, Derivative Assay, Purification, Produced

Journal: Molecular Therapy Advances

Article Title: Manufacture of adeno-associated virus vectors by a novel human-derived cell line HAT and comprehensive evaluation of the vectors

doi: 10.1016/j.omta.2026.201700

Figure Lengend Snippet: Comparison of transduction efficiency across producing cell lines and rAAV purification methods (A–C) Transduction efficiency comparing the HAT- and HEK293-derived products of AAV2 (A), AAV5 (B), and AAV9 (C). (D) Transduction efficiency comparing DGUC- and AEX-purified HAT-cell-produced AAV9. Lines use the same color-coding as in . Error bars are ±SD of the mean of triplicate experiments.

Article Snippet: For the resDNASEQ Quantitative HEK293 DNA Kit, the standard curve was generated using Quantitative HEK293 Genomic DNA (1592106, American Type Culture Collection).

Techniques: Comparison, Transduction, Purification, Derivative Assay, Produced

Journal: Molecular Therapy Advances

Article Title: Manufacture of adeno-associated virus vectors by a novel human-derived cell line HAT and comprehensive evaluation of the vectors

doi: 10.1016/j.omta.2026.201700

Figure Lengend Snippet: Comparison of post-translational modifications between HAT- and HEK293-cell-derived products (A and B) Bar graphs showing the analytical results for the purified products derived from HAT and HEK293 cells: N56/57 deamination (A) and N93/94 deamidation (B). Error bars are ±SD of the mean of triplicate experiments. (C–E) Lectin microarray analysis comparing HAT- and HEK293-cell-derived products of AAV2 (C), AAV5 (D), and AAV9 (E). The net intensity value for each spot was calculated by subtracting the background value from the signal intensity values of three spots. Bars use the same color-coding as in .

Article Snippet: For the resDNASEQ Quantitative HEK293 DNA Kit, the standard curve was generated using Quantitative HEK293 Genomic DNA (1592106, American Type Culture Collection).

Techniques: Comparison, Derivative Assay, Purification, Microarray

Journal: Molecular Therapy Advances

Article Title: Manufacture of adeno-associated virus vectors by a novel human-derived cell line HAT and comprehensive evaluation of the vectors

doi: 10.1016/j.omta.2026.201700

Figure Lengend Snippet: Comparison of murine gene transfer potency between the HAT- and HEK-cell-derived products (A and B) Comparison of in vivo gene transfer efficiency of AAV5 (A) and AAV9 (B) derived from HAT and HEK293 cells in mouse liver. Enhanced green fluorescent protein (EGFP) expression in the liver was evaluated using quantitative data for the EGFP-positive cells in immunostaining. (C and D) Comparison of mRNA expression in various mouse organs by the HAT products and HEK293 products of AAV5 (C) and AAV9 (D). A relative quantity of mRNA expression from HEK-cell-produced AAV in liver was set to 1. (E and F) Comparison of genomic DNA in each organ by the HAT products and HEK293 products of AAV5 (E) and AAV9 (F). (G) Comparison of EGFP fluorescence intensity after bilateral injection of the HAT- or HEK-cell-produced rAAV9 into the mouse motor cortex. The average intensity from HEK-cell-produced AAV was set to 1. Each closed circle represents the value from one hemisphere ( n = 4 hemispheres from two mice). n.s., not significant by unpaired t test. Error bars indicate SD of experimental replicates. (H) Proportions of EGFP-positive cell types. The percentages of neurons (NeuN-positive), microglia (Iba1-positive), astrocytes (GFAP-positive), and oligodendrocytes (Olig2-positive only) among EGFP-expressing cells are shown. Each closed circle represents the value from one hemisphere ( n = 4 hemispheres from two mice). n.s., not significant by unpaired t test. Error bars indicate SD of experimental replicates. Bars use the same color-coding as in .

Article Snippet: For the resDNASEQ Quantitative HEK293 DNA Kit, the standard curve was generated using Quantitative HEK293 Genomic DNA (1592106, American Type Culture Collection).

Techniques: Comparison, Derivative Assay, In Vivo, Expressing, Immunostaining, Produced, Fluorescence, Injection