Journal: Thoracic Cancer

Article Title: circ‐ TTC17 Promotes Esophagus Squamous Cell Carcinoma Cell Growth, Metastasis, and Inhibits Autophagy‐Mediated Radiosensitivity Through miR ‐145‐5p/ SIRT1 Axis

doi: 10.1111/1759-7714.15494

Figure Lengend Snippet: The expression of circ‐TTC17 and miR‐145‐5p in ESCC. (A–B) QRT‐PCR results indicated that circ‐TTC17 was highly expressed in ESCC tissues (T) and cell lines compared with that in adjacent normal tissues (N) and HEEC cells, respectively. * p < 0.05.

Article Snippet: ESCC cell lines (TE1, KYSE150, KYSE180, and KYSE30) and human esophageal epithelial cells (HEEC) were purchased from Procell (Wuhan, China) and grown in RPMI‐1640 medium containing 10% FBS and 1% penicillin/streptomycin. circ‐TTC17 siRNA, lentiviral shRNA, miR‐145‐5p mimic, anti‐miR‐145‐5p, and negative controls were transfected into KYSE180 and KYSE30 cells by Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: Thoracic Cancer

Article Title: circ‐ TTC17 Promotes Esophagus Squamous Cell Carcinoma Cell Growth, Metastasis, and Inhibits Autophagy‐Mediated Radiosensitivity Through miR ‐145‐5p/ SIRT1 Axis

doi: 10.1111/1759-7714.15494

Figure Lengend Snippet: miR‐145‐5p targeted SIRT1 in ESCC. (A) The binding sequences between miR‐145‐5p and SIRT1 3′UTR were presented. The interaction was verified using dual‐luciferase reporter assay (B–C) and RIP assay (D–E). (F) QRT‐PCR was used to detect SIRT1 mRNA expression in ESCC tissues (T) and adjacent normal tissues (N). (G) The correlation between SIRT1 and miR‐145‐5p expression was assessed by Pearson correlation coefficient analysis. (H) The protein expression of SIRT1 in T and N was determined by WB analysis. The mRNA and protein levels of SIRT1 in ESCC cell lines and HEEC cells were measured using qRT‐PCR (I) and WB analysis (J), respectively. (K) The transfection efficiency of miR‐145‐5p mimic and inhibitor was evaluated using qRT‐PCR. (L) SIRT1 protein expression in KYSE180 and KYSE30 cells was determined by WB analysis. * p < 0.05.

Article Snippet: ESCC cell lines (TE1, KYSE150, KYSE180, and KYSE30) and human esophageal epithelial cells (HEEC) were purchased from Procell (Wuhan, China) and grown in RPMI‐1640 medium containing 10% FBS and 1% penicillin/streptomycin. circ‐TTC17 siRNA, lentiviral shRNA, miR‐145‐5p mimic, anti‐miR‐145‐5p, and negative controls were transfected into KYSE180 and KYSE30 cells by Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).

Techniques: Binding Assay, Luciferase, Reporter Assay, Quantitative RT-PCR, Expressing, Transfection