Journal: BMC Genomics

Article Title: Lipid metabolism-related genes are involved in the occurrence of asthma and regulate the immune microenvironment

doi: 10.1186/s12864-023-09795-3

Figure Lengend Snippet: GSEA enrichment analysis. A - C GSEA enrichment plots of hub genes ( ASAH1 , ACER3 and SGPP1 ). GSEA: gene set enrichment analysis

Article Snippet: Samples were incubated with anti-rabbit ASAH1 antibody (1:80, Proteintech, Chicago, United States), anti-rabbit ACER3 (1:200, Proteintech, Chicago, United States), and anti-rabbit -SGPP1 antibody (1:200, Affinity, Ancaster, Canada).

Techniques:

Journal: BMC Genomics

Article Title: Lipid metabolism-related genes are involved in the occurrence of asthma and regulate the immune microenvironment

doi: 10.1186/s12864-023-09795-3

Figure Lengend Snippet: Correlation analysis between hub genes and 22 immune cells. A - C The lollipop diagram showing the relationship of hub genes ( ASAH1 , ACER3 and SGPP1 ) and immune cells

Article Snippet: Samples were incubated with anti-rabbit ASAH1 antibody (1:80, Proteintech, Chicago, United States), anti-rabbit ACER3 (1:200, Proteintech, Chicago, United States), and anti-rabbit -SGPP1 antibody (1:200, Affinity, Ancaster, Canada).

Techniques:

Journal: BMC Genomics

Article Title: Lipid metabolism-related genes are involved in the occurrence of asthma and regulate the immune microenvironment

doi: 10.1186/s12864-023-09795-3

Figure Lengend Snippet: Correlation of ASAH1 with differentially-expressed immune cells. A , B Association between ASAH1 and CD8 + T cell and activated NK cells

Article Snippet: Samples were incubated with anti-rabbit ASAH1 antibody (1:80, Proteintech, Chicago, United States), anti-rabbit ACER3 (1:200, Proteintech, Chicago, United States), and anti-rabbit -SGPP1 antibody (1:200, Affinity, Ancaster, Canada).

Techniques:

Journal: BMC Genomics

Article Title: Lipid metabolism-related genes are involved in the occurrence of asthma and regulate the immune microenvironment

doi: 10.1186/s12864-023-09795-3

Figure Lengend Snippet: Correlation of ASAH1 with differentially-expressed immune cells. A , B Association between ASAH1 and monocytes and M0 macrophages

Article Snippet: Samples were incubated with anti-rabbit ASAH1 antibody (1:80, Proteintech, Chicago, United States), anti-rabbit ACER3 (1:200, Proteintech, Chicago, United States), and anti-rabbit -SGPP1 antibody (1:200, Affinity, Ancaster, Canada).

Techniques:

Journal: BMC Genomics

Article Title: Lipid metabolism-related genes are involved in the occurrence of asthma and regulate the immune microenvironment

doi: 10.1186/s12864-023-09795-3

Figure Lengend Snippet: The result of validation by immunofluorescent staining and RT-qPCR in mouse models ( A – D ) immunofluorescent staining images of lung tissues and quantification of fluorescence intensity, Nucleus (blue), ASAH1/ACER3/SGPP1 (red), scale bar, 500 um, ( n = 4 per group) ( E – G ) The expression of ASAH1 , ACER3 , and SGPP1 verified by RT- qPCR in the OVA model ( n = 4 per per)

Article Snippet: Samples were incubated with anti-rabbit ASAH1 antibody (1:80, Proteintech, Chicago, United States), anti-rabbit ACER3 (1:200, Proteintech, Chicago, United States), and anti-rabbit -SGPP1 antibody (1:200, Affinity, Ancaster, Canada).

Techniques: Biomarker Discovery, Staining, Quantitative RT-PCR, Fluorescence, Expressing

Journal: iScience

Article Title: Serum Deprivation of Mesenchymal Stem Cells Improves Exosome Activity and Alters Lipid and Protein Composition.

doi: 10.1016/j.isci.2019.05.029

Figure Lengend Snippet: Figure 4. Dilysocardiolipin Enrichment in Stressed Exosomes Contributes to Improved Trafficking to Neurons Exosomes purified from umbilical cord-derived mesenchymal stem cells under control conditions or stress conditions (serum deprivation) underwent MS/MSALL lipidomics analysis. N = 2–5 biological replicates were analyzed per group. (A) Heatmap of lipid classes in stressed conditions versus control conditions. Orange represents enrichment in ExosomesStressed versus ExosomesControl, MicrovesiclesStressed versus MicrovesiclesControl, and CellsStressed versus CellsControl, whereas blue represents enrichment in control conditions versus stress conditions. (B) Scheme of cardiolipin. Length and saturation of fatty acid tails depicted is representative only and varies between natural cardiolipin species. (C) Scheme of monolysocardiolipin. Differences to cardiolipin are shown in red. Length and saturation of fatty acid tails depicted are representative only and vary between natural monolysocardiolipin species. (D) Scheme of dilysocardiolipin. Differences to cardiolipin are shown in red. Length and saturation of fatty acid tails depicted are representative only and vary between natural dilysocardiolipin species. (E)Enrichmentofcardiolipinsubclassesfrom(B–D)inExosomesStressedversusExosomesControl(darkorange),MicrovesiclesStressed

Article Snippet: Cardiolipin containing liposomes: Cariolipin (#840012, Avanti Polar Lipids, Alabaster, AL), monolysocardiolipin (#850081, Avanti Polar Lipids, Alabaster, AL) and dilysocardiolipin 21 (#850082, Avanti Polar Lipids, Alabaster, AL) were diluted in chloroform at a concentration of 10 mg/m.

Techniques: Derivative Assay, Control