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AutoMate Scientific Inc
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ADInstruments
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Proteintech
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OriGene
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Addgene inc
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Revvity
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TSE systems
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OriGene
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Proteintech
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Eppendorf AG
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DSMZ
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Eppendorf AG
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Image Search Results
Journal: bioRxiv
Article Title: Gli2 Facilitates Tumor Immune Evasion and Immunotherapeutic Resistance by Coordinating Wnt Ligand and Prostaglandin Signaling
doi: 10.1101/2024.03.31.587500
Figure Lengend Snippet: ( A ) Depiction of canonical via Smoothened (SMO) and non-canonical activation of Gli2. ( B ) Spearman correlation of Gli2 expression with WNT5A, FOXL1, and FOXC2 in human melanoma based on TCGA. ( C ) Expression of FoxL1 and FoxC2 in tumor tissues from an autochthonous melanoma model following anti-PD-1 antibody escape based on RNAseq (n = 3 tumors/group). ( Left ) Expression of FOXL1 and FOXC2 in human melanoma tissues based on RNAseq ( Right ) ( D ) Western blot of the active form of Gli2 after anti-PD-1 escape in the murine BRAF V600E PTEN −/− melanoma model. Representative of 3 independent experiments. ( E ) Transcriptional Nanostring analysis of melanoma tissues derived from stage IV melanoma patients undergoing anti-PD-1 therapy. R, responder. LR, late relapse. NR, nonresponder. ab, antibody. Data presented as mean ± SEM. Two group comparisons analyzed based on unpaired student’s t test. * p <0.05, *** p <0.001, **** p <0.0001.
Article Snippet: The following antibodies were used: IDO1 (mIDO-48, Santa Cruz Biotechnology, sc-53978), Gli2 (Abcam, ab7195, ab277800), Zeb1 (Cell Signaling, D80D3), Fn1 (Thermo Fisher, MA5-11981), Wnt5a (Santa Cruz, sc-365370), Cox2 (Cell Signaling, D5H5 clone, 12282), FoxL1 (invitrogen, PA5-40518),
Techniques: Activation Assay, Expressing, Western Blot, Derivative Assay
Journal: bioRxiv
Article Title: Gli2 Facilitates Tumor Immune Evasion and Immunotherapeutic Resistance by Coordinating Wnt Ligand and Prostaglandin Signaling
doi: 10.1101/2024.03.31.587500
Figure Lengend Snippet: ( A ) Western blot analysis of Wnt5a in a BRAF V600E PTEN −/− Gli2 CA melanoma cell line. ( B ) Western blot analysis of Wnt5a in a wild-type BRAF V600E PTEN −/− melanoma cell line treated with the direct Gli2 inhibitor, Gant61. ( C ) Gene Ontogeny (GO) Pathway Analysis of the BRAF V600E PTEN −/− Gli2 CA melanoma cell line compared to a control BRAF V600E PTEN −/− cell line (NTC). Number of significantly upregulated genes adjacent to GO term. ( D ) Western blot analysis of FoxC2 and FoxL1 transcription factors in the BRAF V600E PTEN −/− Gli2 CA melanoma cell line. ( E ) Tumor growth measurements of BRAF V600E PTEN −/− NTC and Gli2 CA melanoma cell lines in C57Bl/6 mice. Flow cytometric analysis of tumor infiltrating ( F ) CD3 + CD8 + T cells, ( G ) PMN-MDSCs, and ( H ) ratio of cDC2s to cDC1s. (n = 6 tumors/group) ( I ) Whole tumor transcriptional analysis of BRAF V600E PTEN −/− NTC and BRAF V600E PTEN −/− Gli2 CA melanomas. NTC, non-target control. CA, constitutively active. Data presented as mean ± SEM. All data representative of 2-3 independent experiments. Data analyzed by E,F,G,H: unpaired student’s t test. * p <0.05 *** p <0.001.
Article Snippet: The following antibodies were used: IDO1 (mIDO-48, Santa Cruz Biotechnology, sc-53978), Gli2 (Abcam, ab7195, ab277800), Zeb1 (Cell Signaling, D80D3), Fn1 (Thermo Fisher, MA5-11981), Wnt5a (Santa Cruz, sc-365370), Cox2 (Cell Signaling, D5H5 clone, 12282), FoxL1 (invitrogen, PA5-40518),
Techniques: Western Blot, Control
Journal: bioRxiv
Article Title: Oxygen production via NO dismutation in different ammonia oxidizers
doi: 10.1101/2023.06.07.544047
Figure Lengend Snippet: Oxygen accumulation (black symbols) coupled to NO (grey symbols) in oxygen-depleted incubations of different AOB isolates. A, B) Nitrosomonas europaea C, D) Nitrosospira multiformis . Oxygen and NO profile show two panels of reproducible replicates (at least 3n per incubation). Pronounced increases in the oxygen concentration of A and B are due to oxygen intrusion associated to oxygenated water injection in the incubation (arrows). Oxygen concentrations were corrected for the interference of NO with the optodes.
Article Snippet: The strain N. piranensis DC3 (JCM 32271, DSM 106147, NCIMB 15115) and N. viennensis EN76 (JCM 19564, DSM 26422) were obtained from the Japan Collection of Microorganisms (JCM), the strains N. adriaticus NF5 (JCM 32270, NCIMB 15114) and Nitrosopumilus sp. CCS1 were obtained from the culture collection of Alyson Santoro at the University of California, Santa Barbara, while the strains N. europaea (DSM 28437) and
Techniques: Incubation, Concentration Assay, Injection