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Image Search Results
Journal: International Journal of Biological Sciences
Article Title: 11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo Signaling
doi: 10.7150/ijbs.82933
Figure Lengend Snippet: 11β-HSD1 expression and osteoclast number increased in ovariectomized (OVX) mice. A. Representative μCT images of 8w-OVX mice femur and the control. B. Quantitative μCT analysis of trabecular bone volume to tissue volume (BV/TV) and trabecular separation (Tb. Sp) of femora. n = 5 per group. C. Representative H&E staining images of 8w-OVX mice femur and the control. D. Representative IF images of 11β-HSD1 (green) and TRAP (red) staining in 8w-OVX mice femur and the control (Left). Quantification of 11β-HSD1 and TRAP staining (Right). (*means P value<0.05, **means P value<0.01)
Article Snippet:
Techniques: Expressing, Control, Staining
Journal: International Journal of Biological Sciences
Article Title: 11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo Signaling
doi: 10.7150/ijbs.82933
Figure Lengend Snippet: High expression of 11β-HSD1 induced osteoporosis in vivo. A. Representative immumohistochemical images of 11β-HSD1 staining in the control, 8w-KO and 8w-KI mice femur (Left). Quantification of 11β-HSD1 staining (Right). B. Representative μCT images of mice femurs from the control, 8w-KO and 8w-KI groups. C. Quantitative μCT analysis of trabecular bone volume to tissue volume (BV/TV) and trabecular separation (Tb. Sp). n = 5 per group. D. Representative H&E staining images of mice femurs from the control, 8w-KO and 8w-KI groups. E. Representative TRAP staining of mice femurs from the control, 8w-KO and 8w-KI groups. (*means P value<0.05, **means P value<0.01)
Article Snippet:
Techniques: Expressing, In Vivo, Staining, Control
Journal: International Journal of Biological Sciences
Article Title: 11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo Signaling
doi: 10.7150/ijbs.82933
Figure Lengend Snippet: 11β-HSD1 promotes osteoclastogenesis in vitro . A. BMMs were isolated from the wild type, KO and KI groups. After induction with osteoclastic differentiation medium for 7 days, TRAP staining was performed to evaluate osteoclast formation. B. Western blot (WB) evaluation of 11β-HSD1 and CTSK expression change in BMMs from the wild type, KO and KI groups. C. RT-qPCR analysis of 11β-HSD1, ACP5, Cath-K and Nfatc-1. D. BVT-2733 with different concentrations were added into osteoclast incubation medium. Trap staining was performed to evaluate the osteoclast formation. (*means P value<0.05, **means P value<0.01)
Article Snippet:
Techniques: In Vitro, Isolation, Staining, Western Blot, Expressing, Quantitative RT-PCR, Incubation
Journal: International Journal of Biological Sciences
Article Title: 11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo Signaling
doi: 10.7150/ijbs.82933
Figure Lengend Snippet: Conditional knock out 11β-HSD1 in osteoclast rescued osteoporosis in vivo . A. Representative μCT images of the femurs from 8w-cKO or wild type mice in OVX conditions. B. Quantitative μCT analysis of BV/TV and Tb. Sp. n = 5 per group. C. Representative H&E staining images of the femurs. D. Representative IF images showing 11β-HSD1 (green) and TRAP (red) staining in the femurs from cKO or wild type mice in OVX conditions (Top). Quantification of 11β-HSD1 and TRAP staining (Bottom). (*means P value<0.05, **means P value<0.01)
Article Snippet:
Techniques: Knock-Out, In Vivo, Staining
Journal: International Journal of Biological Sciences
Article Title: 11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo Signaling
doi: 10.7150/ijbs.82933
Figure Lengend Snippet: 11β-HSD1 suppressed H type vessel formation. A. Representative IF images of Endomucin (Emcn, red), CD31 (green) and DAPI (blue). B. Quantitative analysis of the area of H type vessel (CD31 hi Emcn hi , yellow) near femoral growth plate in control, KO and KI groups. C. Representative images of tube formation assay with the supernatant of osteoclast induction medium from the control, KO and KI groups. D. Representative IF images of TRAP (red), PDGF-BB (green) and DAPI (blue). E. Quantitative analysis of the area of TRAP with PDGF-BB. F. PDGF-BB Elisa analysis of the supernatant in osteoclast induction from the control, KO and KO group.
Article Snippet:
Techniques: Control, Tube Formation Assay, Enzyme-linked Immunosorbent Assay
Journal: International Journal of Biological Sciences
Article Title: 11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo Signaling
doi: 10.7150/ijbs.82933
Figure Lengend Snippet: 11β-HSD1 promotes osteoclastogenesis through Hippo signaling pathway. A. Transcriptome analysis of the BMMs isolated from the control and KI femurs. B. Representative IF images of TRAP (red), YAP (green) and DAPI (blue). C. Quantitative analysis of the area of TRAP colocalized with YAP (yellow) near femoral growth plate. D. Representative immumohistochemical images showing YAP in the femors from control, KO and KI groups. E. Quantification analysis of YAP staining. F. WB evaluation of 11β-HSD1 and YAP expression change in BMMs under verteporfin conditions. G. Verteporfin was added into osteoclast incubation medium. TRAP staining was performed to evaluate the osteoclast formation (Left) and the quantitative analysis (Right).
Article Snippet:
Techniques: Isolation, Control, Staining, Expressing, Incubation
Journal: International Journal of Biological Sciences
Article Title: 11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo Signaling
doi: 10.7150/ijbs.82933
Figure Lengend Snippet: Sequences of primers employed for RT-PCR
Article Snippet:
Techniques: Sequencing
Journal: International Journal of Biological Sciences
Article Title: 11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo Signaling
doi: 10.7150/ijbs.82933
Figure Lengend Snippet: Sequences of primers employed for PCR
Article Snippet:
Techniques: Sequencing
Journal: International Journal of Biological Sciences
Article Title: 11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo Signaling
doi: 10.7150/ijbs.82933
Figure Lengend Snippet: Antibodies used in this study
Article Snippet:
Techniques: