Journal: PLoS ONE

Article Title: Proteomic Profiling of Human Keratinocytes Undergoing UVB-Induced Alternative Differentiation Reveals TRIpartite Motif Protein 29 as a Survival Factor

doi: 10.1371/journal.pone.0010462

Figure Lengend Snippet: A: Immunofluorescent staining of TRIM29 (green) of histological sections of reconstructed epidermis and from human skin tissue were visualized by semi-quantitative confocal microscopy. Nuclei were stained with TO-PRO-3 (blue). The results are representative of three independent experiments. B: TRIM29 mRNA abundance is more important in keratinocytes than in other cell types. Total RNA was extracted from subconfluent N-hTERT and primary keratinocytes, from dermal fibroblasts AG04431 and from hepatocarcinoma cells (HepG2). Real-time RT-PCR was performed for human TRIM29. TRIM29 mRNA abundance in fibroblasts was considered as the reference. The results (means ± SD from triplicates) (N = 3) are presented on a logarithmic scale (** p<0.01 and *** p<0.001 vs fibroblasts).

Article Snippet: Human dermal fibroblasts AG04431 (Coriell Institute for medical Research, Camden NJ, USA) were grown classically in BME (Invitrogen, Carlsbad, CA, USA) containing 10% (v:v) fetal bovine serum (Invitrogen, Carlsbad, CA, USA) and 2 mM L-glutamine (Invitrogen, Carlsbad, CA, USA), under 5% CO 2 .

Techniques: Staining, Confocal Microscopy, Quantitative RT-PCR