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Image Search Results
Journal: iScience
Article Title: miR-376a-3p and miR-376b-3p overexpression in Hutchinson-Gilford progeria fibroblasts inhibits cell proliferation and induces premature senescence
doi: 10.1016/j.isci.2022.103757
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Transfection, Staining, Enzyme-linked Immunosorbent Assay, Cytotoxicity Assay, Viability Assay, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Expressing, Negative Control, Sequencing, Plasmid Preparation, Software
Journal: Molecular Therapy. Nucleic Acids
Article Title: Base editing rescues acid α-glucosidase function in infantile-onset Pompe disease patient-derived cells
doi: 10.1016/j.omtn.2024.102220
Figure Lengend Snippet: Edited human dermal fibroblasts have restored GAA enzyme activity and reduced LAMP2 (A) GAA enzyme activity at 4, 14, and 28 days post-nucleofection in IOPD-1, -2, and -3 dermal fibroblast populations compared to mock cells. Comparisons were analyzed using two-tailed unpaired Student's t tests; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. Yellow shading represents the normal range of GAA activity in cultured unaffected HDFs (45–180 units/mg protein). (B) Western blots of IOPD-1, -2, and -3 (−/−), ABE-treated IOPD-1, -2, and -3 (edited), and unaffected (+/+) HDFs using antibodies to detect GAA, LAMP2, and GAPDH. Quantification of western blots for LAMP2 and GAA normalized to GAPDH and expressed as relative density (fold normal). Comparisons were analyzed using one-way ANOVA with Tukey post-hoc test; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, and ns, not significant. (C) Immunofluorescence photomicrographs showing LAMP2 signal (green) in IOPD-1, -2, and -3 (−/−), ABE-treated IOPD-1, -2, and -3 (edited), and unaffected (+/+) HDFs. Scale bar represents 50 μm. All error bars show mean ± standard deviation.
Article Snippet: IOPD-2 primary
Techniques: Activity Assay, Two Tailed Test, Cell Culture, Western Blot, Immunofluorescence, Standard Deviation