Journal: PLoS Genetics

Article Title: Smad2 and Smad3 Regulate Chondrocyte Proliferation and Differentiation in the Growth Plate

doi: 10.1371/journal.pgen.1006352

Figure Lengend Snippet: (A) Immunohistochemical staining of Hdac4 in E18.5 proximal tibias. Bottom panel shows magnifications of the boxed regions in top panel, representing the lower columnar and prehypertrophic zones. (B) ChIP analysis of Hdac4 binding to SBE 1–3 in ATDC5 cells. (C and D) Immunohistochemical staining of SnoN and Ski in E18.5 proximal tibias. Asterisks in (C and D) indicated SnoN or Ski present in columnar zone cells. (E) ChIP analysis of SnoN and Ski binding to SBE 1–3 in ATDC5 cells. Cells in (B and E) were matured to prehypertrophy and subsequently treated with TGFβ1 (5 ng/ml) or non-treated (Control) for 24 hrs. Asterisk in (B and E), p = or < 0.05. All experiments were performed in triplicate and repeated twice. Smad2 CKO = Smad2 fx/fx ;Col2a1Cre .

Article Snippet: Antibodies used were: phospho-Smad2 (Cell Signaling, Beverly, MA, USA, #3108), PCNA (Cell Signaling, #13110), Ihh (Abcam, ab52919), Patched1 (Novus Biologicals, NB200-118), Collagen X (Abcam, ab140230), SnoN (Santa Cruz, sc-9141), Ski (Santa Cruz, sc-9140) and Hdac4 (Cell Signaling, cs-2072).

Techniques: Immunohistochemical staining, Staining, Binding Assay, Control

Journal: Cell Death & Disease

Article Title: Deacetylation of TALDO1 by HDAC6 promotes glycolysis and nasopharyngeal carcinoma progression through a moonlighting function

doi: 10.1038/s41419-025-08057-2

Figure Lengend Snippet: A – C After indicated plasmids were transfected into 293T cells for 48 h, A Interaction between HDAC4/5/6 and TALDO1 was detected by IP and western blot. B , C TALDO1 acetylation was detected by IP and western blot. D After transfected Flag-TALDO1 in NPC cells, interaction between exogenous Flag-TALDO1 and endogenous HDAC6 was analyzed by IP and western blot. E Indicated plasmids were transfected into HK1 cells, and the colocalization of TALDO1 and HDAC6 was investigated by confocal microscopy. Scale bar, 5 μm. F Indicated plasmids were transfected into 293T cells, TALDO1 acetylation was detected by IP and western blot. G After established HK1, SUNE1 and HONE1 knockdown HDAC6 stable cell lines through transfected shHDAC recombinant lentivirus, western blot analysis of TALDO1 expression in HDAC6 stable knockdown NPC cells. H After co-transfected Flag-TALDO1 and Myc-p300 plasmids in 293T cells, and interaction between TALDO1 and p300 was detected by IP and western blot. I The endogenous interaction between TALDO1 and p300 was detected by IP and western blot. J After co-transfected Flag-TALDO1 and Myc-p300 plasmids in HK1 cells, the colocalization of TALDO1 and p300 was investigated by confocal microscopy. Scale bar, 5 μm. K After co-transfected Flag-TALDO1 and Myc-p300 plasmids in 293T cells, TALDO1 acetylation was detected by IP and western blot. L After co-transfected Flag-TALDO1 and Myc-p300 WT or DY (a catalytic inactivation mutant of p300) plasmids in 293T cells, TALDO1 acetylation was analyzed by IP and western blot. M Western blot analysis of TALDO1 in p300 overexpression NPC cells. N After co-transfected Flag-TALDO1 and Myc-p300 followed by transfection with His-HDAC6 expression plasmid for 48 h, TALDO1 acetylation was detected by IP and western blot. O The expression and correlation of TADLO1 and HDAC6 or p300 were analyzed by IHC staining of clinical NPC tissues. Sample size ( n = 35), Pearson correlation coefficient (r) and p -value (two-tailed) are indicated.

Article Snippet: Primary antibodies used were TALDO1 (12376-1-AP), Flag-tag (80010-1-RR), HDAC4 (17449-1-AP), HDAC5 (16166-1-AP), HDAC6 (12834-1-AP), His-tag (66005-1-Ig), HA-tag (66006-2-Ig), Myc-tag (16286-1-AP), p300 (20695-1-AP), BRCA1 (22362-1-AP), and c-Myc (10828-1-AP) from Proteintech (Chicago, IL); SMURF1 (sc-100616) and SYVN1 (sc-293484) from Santa Cruz Biotechnology (Santa Cruz, CA); Actin (AC026) from Abclonal (Wuhan, China).

Techniques: Transfection, Western Blot, Confocal Microscopy, Knockdown, Stable Transfection, Recombinant, Expressing, Mutagenesis, Over Expression, Plasmid Preparation, Immunohistochemistry, Two Tailed Test