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Image Search Results
Journal: Respiratory Research
Article Title: Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung
doi: 10.1186/s12931-021-01679-y
Figure Lengend Snippet: Scheme of the molecular interactions of TBX2 in repression of target genes in the pulmonary mesenchyme. Grey discs represent nucleosomes. DNA is displayed as black line. Dotted line indicates unknown length of DNA fragment and possible long distance. Circles for proteins do not represent the real size. TBX2 binds in concert with HMGB2 and PBX1 to specific DNA sites in enhancer regions. Binding to PBX1 is probably indirect via unknown proteins, binding to HMGB2 is direct and may aid in chromatin bending and bringing enhancer regions to the proximity of transcription start sites. TBX2/DNMT1 binding may induce DNA methylation to repress gene expression. TBX2 and CBX3 directly bind and both associate with the NuRD complex for chromatin remodeling. TBX2/MYBBP1 interaction may strengthen the association with the NuRD complex to repress target genes. TBX2 directly interacts with components of the NuRD complex, namely HDACs for chromatin deacetylation and the chromatin remodeling complex CHD4. TBX2 interaction with CBX3, HDACs and CHD4 collectively induces chromatin compaction. RBBP4/7 binds to chromatin to consolidate binding of the NuRD-complex to target gene DNA
Article Snippet: We used the following expression vectors for transfections in HEK293 cells: pcDNA3.huTBX2.HA encoding N-terminally HA-tagged full-length human TBX2; pCS2.Pbx1b encoding full-length mouse PBX1B , (gift from Heike Pöpperl, Institute for Biophysical Chemistry, Hannover Medical School, Germany); pd2EGFP-N1 (EGFP-expression vector) [ ]; pCMV6-Entry.Cbx3-Myc-DDK encoding full-length mouse CBX3 (#MR224357, OriGene, Rockville, Maryland, USA); pCMV-SPORT6.Hdac1 encoding full-length mouse HDAC1 (#4217199, Sourcebioscience, Nottingham, UK); pCMV6-Entry.Hdac2-Myc-DDK encoding full-length mouse HDAC2 (#MR226709,
Techniques: Binding Assay, DNA Methylation Assay, Expressing