Journal: Stem cells (Dayton, Ohio)

Article Title: Mesenchymal Wnt/β-Catenin Signaling Controls Epithelial Stem Cell Homeostasis in Teeth by Inhibiting the Antiapoptotic Effect of Fgf10.

doi: 10.1002/stem.1972

Figure Lengend Snippet: Figure 1. BIO (WNT/b-catenin activator) causes thinning of the LaCL of mouse incisor ex vivo. (A, B): Schematic illustration of the mouse incisor: sagittal cross section of mandible (A) and higher magnification of the labial cervical loop (B). (C, D): The proximal ends of the P2 Sox2-GFP mouse incisor were cultured as explants ex vivo. BIO was diluted in DMSO and used in 15 mM concentration, and the same amount of DMSO was supplemented in the controls. The LaCLs of the explants cultured with BIO were significantly thinner than in controls after 1 day culture (p < 0.01) and the thinning was more obvious after 2 days (p < 0.01). Scale bar 5 50 mm. Abbrevia- tions: Am, ameloblasts; IEE, inner enamel epithelium; LaCL, labial cervical loop; LiCL, lingual cervical loop; OEE, outer enamel epithelium; SR, stellate reticulum.

Article Snippet: Nonspecific staining was blocked by incubation in 10% normal donkey serum and 0.5% Triton X-100 in PBS O/N at 4 C. Explants were incubated with the following primary antibodies at 4 C for 2 days: Sox2 1:400 (sc-17320, Santa Cruz, Dallas, TX, USA, http://www.scbt.com/), Caspase 3 1:400 (9661, Cell Signaling Technology, Danvers, MA, USA, http:// www.cellsignal.com/), b-catenin 1:1,000 (610154, BD Transduction Laboratories, San Jose, CA, USA, http://www. bdbiosciences.com), and GFP (ab13970, Abcam, Cambridge, England, United Kingdom, abcam.com).

Techniques: Ex Vivo, Cell Culture, Concentration Assay