Journal: Molecular Vision

Article Title: Enhanced survival in vitro of human corneal endothelial cells using mouse embryonic stem cell conditioned medium

doi:

Figure Lengend Snippet: Cell number in three different mediums. The HCECs were seeded at the density of 9×10 4 cells/well on 12-well plates. The ESC-CM and ESC medium were added to passage 1 cells. Data are expressed as the mean±SEM (n=3). The values with the same letter (a, b, c, d) in each column indicate these values are not significantly different (p>0.05). The same indication is used in all subsequent figures.

Article Snippet: The cell-cycle entrance of HCECs treated with 25%ESC-CM was significantly higher than that of CEM group both in passage 2 (p=0.001) and passage 4 (p=0.000).

Techniques:

Journal: Molecular Vision

Article Title: Enhanced survival in vitro of human corneal endothelial cells using mouse embryonic stem cell conditioned medium

doi:

Figure Lengend Snippet: The morphology and cell size of HCECs cultured in the CEM group and the 25%ESC-CM group. The 25%ESC-CM group maintained the morphology and cell size of HCECs until passage (P6).

Article Snippet: The cell-cycle entrance of HCECs treated with 25%ESC-CM was significantly higher than that of CEM group both in passage 2 (p=0.001) and passage 4 (p=0.000).

Techniques: Cell Culture

Journal: Molecular Vision

Article Title: Enhanced survival in vitro of human corneal endothelial cells using mouse embryonic stem cell conditioned medium

doi:

Figure Lengend Snippet: Increased frequency of proliferating HCECs in conditioned medium from mouse embryonic stem cells. A : Passage 4 HCECs were cultured for 48 h and probed for Ki67 expression. B : Ki67-positive cells were analyzed by flow cytometry. Flow cytometry analysis revealed significantly increased Ki67-positive HCECs in the 25%ESC-CM group (p=0.000). Data are expressed as the mean ± SEM (n=3).

Article Snippet: The cell-cycle entrance of HCECs treated with 25%ESC-CM was significantly higher than that of CEM group both in passage 2 (p=0.001) and passage 4 (p=0.000).

Techniques: Cell Culture, Expressing, Flow Cytometry

Journal: Molecular Vision

Article Title: Enhanced survival in vitro of human corneal endothelial cells using mouse embryonic stem cell conditioned medium

doi:

Figure Lengend Snippet: Conditioned medium from mouse embryonic stem cells stimulated colony formation of HCECs. The colony formation was significantly higher in the 25%ESC-CM group than in the CEM group (p=0.000). Data are expressed as the mean ± SEM (n=5).

Article Snippet: The cell-cycle entrance of HCECs treated with 25%ESC-CM was significantly higher than that of CEM group both in passage 2 (p=0.001) and passage 4 (p=0.000).

Techniques:

Journal: Molecular Vision

Article Title: Enhanced survival in vitro of human corneal endothelial cells using mouse embryonic stem cell conditioned medium

doi:

Figure Lengend Snippet: Conditioned medium from mouse embryonic stem cells promoted cell-cycle entrance of HCECs. The percentage of S phase and G 2 phase cells in 25%ESC-CM group was significantly increased at passage 2 (p = 0.001) and passage 4 (p=0.000). Data are expressed as the mean ± SEM (n=3).

Article Snippet: The cell-cycle entrance of HCECs treated with 25%ESC-CM was significantly higher than that of CEM group both in passage 2 (p=0.001) and passage 4 (p=0.000).

Techniques:

Journal: Molecular Vision

Article Title: Enhanced survival in vitro of human corneal endothelial cells using mouse embryonic stem cell conditioned medium

doi:

Figure Lengend Snippet: Conditioned medium from mouse embryonic stem cells inhibited the apoptosis of HCECs. The apoptosis/necrosis rate of passage 4 HCECs in 25%ESC-CM group was significantly lower than in CEM group (p=0.001). Data are expressed as the mean ± SEM (n=3).

Article Snippet: The cell-cycle entrance of HCECs treated with 25%ESC-CM was significantly higher than that of CEM group both in passage 2 (p=0.001) and passage 4 (p=0.000).

Techniques:

Journal: Nanomaterials

Article Title: Engineering of Corneal Tissue through an Aligned PVA/Collagen Composite Nanofibrous Electrospun Scaffold

doi: 10.3390/nano8020124

Figure Lengend Snippet: The proliferation results of the HKs and HCECs: ( a ) proliferation of HKs on the 7% aligned collagen and the 9% PVA-COL (aligned and non-aligned) electrospun scaffolds within seven days; ( b ) proliferation histogram of the HKs in the different electrospun scaffolds cultured for seven days; ( c ) proliferation of HCECs on the 7% aligned collagen and the 9% PVA-COL (aligned and non-aligned) electrospun scaffolds within seven days; and ( d ) proliferation histogram of the HCECs in the different electrospun scaffolds cultured for seven days. The control group was the cells that were seeded on the culture plate, the data represent the means ± SD (* p < 0.05).

Article Snippet: HCECs [ , , , , ] were purchased from the RIKEN BioResource Center (Tsukuba, Japan), and HKs [ , ] were obtained from the He Eye Hospital (Shenyang, China).

Techniques: Cell Culture, Control

Journal: Molecular Medicine

Article Title: Oxidized LDL promotes EMS-induced angiogenesis by increasing VEGF-A expression and secretion by endometrial cells

doi: 10.1186/s10020-022-00582-6

Figure Lengend Snippet: EMS induced angiogenesis in endometrial tissue. A The microvessel density in normal endometrium, eutopic endometrium and ectopic endometrium. The microvasculature was stained for CD34 by immunohistochemistry, while all the tissues were also stained by H&E and iron. The number in each group was 8. B Tube formation of hUVECs induced by hESCs and hEECs in a coculture system with chambers. C Histogram representing the microvessel density in normal endometrium, eutopic endometrium and ectopic endometrium. The number in each group was 8. D Histogram representing the tube number in each group. The number in each group was 3. * represents p < 0.05

Article Snippet: Primary hEECs, hESCs and HUVECs were purchased from Procell Biotech (Wuhan, China).

Techniques: Staining, Immunohistochemistry

Journal: Molecular Medicine

Article Title: Oxidized LDL promotes EMS-induced angiogenesis by increasing VEGF-A expression and secretion by endometrial cells

doi: 10.1186/s10020-022-00582-6

Figure Lengend Snippet: OxLDL promoted EMS-induced angiogenesis. A The plasma oxLDL level was elevated in EMS patients. The number in each patient group was 8. B , C Tube formation induced by hESCs and hEECs treated with oxLDL and PBS. The histogram represents the tube number in each group. * represents p < 0.05

Article Snippet: Primary hEECs, hESCs and HUVECs were purchased from Procell Biotech (Wuhan, China).

Techniques: Clinical Proteomics

Journal: Molecular Medicine

Article Title: Oxidized LDL promotes EMS-induced angiogenesis by increasing VEGF-A expression and secretion by endometrial cells

doi: 10.1186/s10020-022-00582-6

Figure Lengend Snippet: OxLDL increased the expression and secretion of VEGF-A in endometrial cells. A VEGF-A expression in eutopic endometrium and ectopic endometrium from the animal models treated with oxLDL (dissolved in PBS solution) or the same volume of PBS for 48 h. VEGF-A was stained by immunohistochemistry, while all the tissues were also stained by H&E and iron. B , C Western blot analysis of VEGF-A expression in the hESCs and hEECs treated with oxLDL and PBS. The histogram represents the greyscale of each lane from the Western blot. The number in each group was 3. * compared with the 0 µg/ml oxLDL group, p < 0.05. D The VEGF-A secretion of the hESCs and hEECs treated with oxLDL and PBS for 48 h was tested by ELISAs. The number in each group was 3. * compared with the 0 µg/ml oxLDL group, p < 0.05

Article Snippet: Primary hEECs, hESCs and HUVECs were purchased from Procell Biotech (Wuhan, China).

Techniques: Expressing, Staining, Immunohistochemistry, Western Blot