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JCRB Cell Bank
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Image Search Results
Journal: mAbs
Article Title: Comparison of newly developed anti-bone morphogenetic protein 4 llama-derived antibodies with commercially available BMP4 inhibitors
doi: 10.1080/19420862.2016.1158380
Figure Lengend Snippet: VHH specificity compared to different inhibitors. C2C12 cells were activated with 50 ng/ml of hBMP2 (A), 10 ng/ml of hBMP4 (B), 200 ng/ml of hBMP5 (C), 50 ng/ml of hBMP6 (D), 200 ng/ml of hBMP7 (E), 25 ng/ml of hBMP9 (F), 25 ng/ml of hBMP10 (G) and 50 ng/ml of hBMP12 (H) for 16h. At the same time the inhibitors were added. VHH, mNoggin, hNoggin and Gremlin were added at a concentration of 500 ng/ml; LDN-193189 (LDN) at 0.5 μM and DMH1 at 0.2 μM.
Article Snippet: To analyze VHH specificity, C2C12 cells were stimulated with 10 ng/ml of hBMP4 (Cat. No. 314-BP-020; R&D Systems), 50 ng/ml of hBMP2 (Cat. No. 355-BM-010; R&D Systems), 200 ng/ml of hBMP5 (Cat. No. 615-BMC-020; R&D Systems), 50 ng/ml of hBMP6 (Cat. No. 506-BP-020; R&D Systems), 200 ng/ml of hBMP7 (Cat. No. 354-BP-010; R&D Systems), 25 ng/ml of hBMP9 (Cat. No. 3209-BP-010; R&D Systems), 25 ng/ml of hBMP10 (Cat. No. 2926-BP-025; R&D Systems), and 50 ng/ml of
Techniques: Concentration Assay