Journal: bioRxiv

Article Title: Identification of lactate as a potential inhibitory factor within epithelial cell supernatant and its effects on type 2 T cell populations

doi: 10.1101/2020.03.26.008292

Figure Lengend Snippet: IL-13 release driven by IL-2 is inhibited in the presence of HBEC cells and epithelial cell CM inhibits release of IL-5 from IL-2 stimulated PBMC. A) Human Bronchial Epithelial cells (HBEC) were cultured in 24 well plates, then PBMC (10 6 ) with IL-2 (200 U/mL) were added in transwells on top. Media controls were 24 well plate wells containing media alone. After 5 days supernatants were collected and IL-13 measured. Values are means +/- SD from three separate wells and three different PBMC donors. B) PBMC were cultured with titrated doses of epithelial cell CM and after five days culture media collected and IL-5 concentration determined. Values are means +/- SD. Data are from one representative experiment repeated at least twice.

Article Snippet: HBEC were obtained from human bronchoscopy samples and cultured in complete HBEC media (Lonza) and put into 24 well plates and used when 90% confluent.

Techniques: Cell Culture, Concentration Assay

Journal: bioRxiv

Article Title: HDAC6 inhibitor ACY-1083 shows lung epithelial protective features in COPD

doi: 10.1101/2022.03.21.485098

Figure Lengend Snippet: Mucociliary transport rates were determined by tracking fluorescent microspheres placed on top of cell layer of unchallenged or TNF-challenged HBEC ALI cultures with or without ACY-1083 treatment. (A) Images of tracked beads in the ImageJ software with the Fiji plugin Tracking. Lines show movement of beads. (B) Microsphere speeds determined from three COPD donors and three fields per well. Box plot with dots representing three COPD donors and whiskers showing min and max. Data was normalized to the DMSO-control without TNF for each donor (set to 100%). Statistical analysis was performed using one-way ANOVA with Dunnett&s multiple comparisons test.

Article Snippet: Primary COPD human bronchial epithelial cells (HBEC) were purchased from Lonza (Basel, Switzerland).

Techniques: Software

Journal: The AAPS Journal

Article Title: Pulmonary Delivery of Aerosolized Chloroquine and Hydroxychloroquine to Treat COVID-19: In Vitro Experimentation to Human Dosing Predictions

doi: 10.1208/s12248-021-00666-x

Figure Lengend Snippet: Schematic representation of the in vitro aerosol generation and exposure system. The aerosol generated passes through a the dilution chamber without any dilution into b the exposure chamber with c trumpet-like outlets to the cell culture inserts. Each cell culture insert contains three-dimensional organotypic human bronchial airway cultures at the air-liquid interface on a porous membrane and culture medium at the bottom

Article Snippet: 3D organotypic HBEC grown at air-liquid interface (ALI) were prepared from primary human bronchial epithelial cells (Lonza, Basel, Switzerland) as previously described by Bovard et al. [ ].

Techniques: In Vitro, Generated, Cell Culture

Journal: The AAPS Journal

Article Title: Pulmonary Delivery of Aerosolized Chloroquine and Hydroxychloroquine to Treat COVID-19: In Vitro Experimentation to Human Dosing Predictions

doi: 10.1208/s12248-021-00666-x

Figure Lengend Snippet: Effects of exposure on a ciliary beating frequency (CBF), b active ciliary beating area, c cellular ATP levels, and d transepithelial electrical resistance (TEER) in three-dimensional organotypic human bronchial airway cells. Data shown before (black bars and dots) and after 24-h (blue bars and dots) exposure to various concentrations of chloroquine and hydroxychloroquine. Data are presented as mean (bars) of three technical replicates (dots) and 95% confidence intervals (error bars). RLU, relative luminescence units

Article Snippet: 3D organotypic HBEC grown at air-liquid interface (ALI) were prepared from primary human bronchial epithelial cells (Lonza, Basel, Switzerland) as previously described by Bovard et al. [ ].

Techniques: