Journal: Cells

Article Title: Highly Efficient Conversion of Motor Neuron-Like NSC-34 Cells into Functional Motor Neurons by Prostaglandin E 2

doi: 10.3390/cells9071741

Figure Lengend Snippet: Expression of motor neuron-specific markers in NSC-34 cells. Undifferentiated NSC-34 cells were treated with a vehicle (EtOH), 30 μM PGE 2 for 2 days, or 10 μM RA for 7 days. Photograph shows a representative result of a Western blot of low-molecular-weight microtubule-associated protein-2 (MAP2c) ( A ), synaptophysin ( B ), HB9 ( C ) and Islet-1 ( D ) with β-actin as an internal marker. Graphs show the relative densities of bands on the blots estimated quantitatively using Scion imaging software. Each value represents the mean ± SD ( n = 3). * p < 0.05, ** p < 0.01.

Article Snippet: The membranes were treated with anti-MAP2 monoclonal antibody (#8707, 1:1000; Cell Signaling Technology, Danvers, MA, USA), anti-Syanaptophysin antibody (#5461, 1:1000; Cell Signaling Technology, Danvers, MA, USA), anti-HB9 antibody (sc-515769, 1:500; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Islet-1 antibody (ab109517, 1:1000, abcam, Cambrige, MA, USA), anti-ChAT antibody (AB144P, 1:500; Millipore, Burlington, MA, USA), and anti-βactin antibody (A5441, 1:2000, Sigma-Aldrich, St. Louis, MO, USA) overnight at 4 °C.

Techniques: Expressing, Western Blot, Molecular Weight, Marker, Imaging, Software

Journal: Journal of Nanobiotechnology

Article Title: Conducting molybdenum sulfide/graphene oxide/polyvinyl alcohol nanocomposite hydrogel for repairing spinal cord injury

doi: 10.1186/s12951-022-01396-8

Figure Lengend Snippet: Primers sequence used for real-time PCR

Article Snippet: The samples were then incubated overnight with primary antibodies: rabbit anti-HB9 antibody (ORB157435, 1:1000, Biorbyt), rabbit anti-Islet-1 antibody (GTX 102807, 1:1000, Genetex), rabbit anti-ChAT antibody (PA5, 1:10000, Thermo Fisher), rabbit anti-GFAP antibody (GTX108711, 1:1000, Genetex), and rabbit anti-Tuj1 (GTX130245, 1:1000, Genetex) at 4 °C.

Techniques: Sequencing

Journal: Journal of Nanobiotechnology

Article Title: Conducting molybdenum sulfide/graphene oxide/polyvinyl alcohol nanocomposite hydrogel for repairing spinal cord injury

doi: 10.1186/s12951-022-01396-8

Figure Lengend Snippet: The growth and differentiation of NE-4C and SH-SY5Y cells after cocultured with PVA, GO/PVA, MoS 2 /PVA, and MoS 2 /GO/PVA hydrogels in vitro. Confocal microscopic images of ( a ) NE-4C cells and b SH-SY5Y cells. Tuj1-positive cells (green) and GFAP-positive cells (red) in different groups (Control, GO/PVA, MoS 2 /PVA, and MoS 2 /GO/PVA hydrogels) at 3 and 7 days. All nuclei were stained with DAPI (blue). Scale bar = 50 μm. c Fluorescence intensity values corresponding to confocal photos of each group. d Quantitative of relative gene expression levels of Tuj1, GFAP, ChAT, Islet-1, and HB9 in NE-4C cells in different groups (Control, GO/PVA, MoS 2 /PVA, and MoS 2 /GO/PVA hydrogels) at 3 and 7 days. Error bars indicate the SD from four different experiments; *p < 0.05, **p < 0.01, all groups were compared with the control group

Article Snippet: The samples were then incubated overnight with primary antibodies: rabbit anti-HB9 antibody (ORB157435, 1:1000, Biorbyt), rabbit anti-Islet-1 antibody (GTX 102807, 1:1000, Genetex), rabbit anti-ChAT antibody (PA5, 1:10000, Thermo Fisher), rabbit anti-GFAP antibody (GTX108711, 1:1000, Genetex), and rabbit anti-Tuj1 (GTX130245, 1:1000, Genetex) at 4 °C.

Techniques: In Vitro, Control, Staining, Fluorescence, Gene Expression

Journal: Journal of Nanobiotechnology

Article Title: Conducting molybdenum sulfide/graphene oxide/polyvinyl alcohol nanocomposite hydrogel for repairing spinal cord injury

doi: 10.1186/s12951-022-01396-8

Figure Lengend Snippet: Differentiation direction of spinal cord tissue in each group. a Representational IHC images of Tuj1, GFAP, ChAT, Islet-1, and HB9 in Sham, SCI, GO/PVA, MoS 2 /PVA, and MoS 2 /GO/PVA groups. The spinal cord tissue of the original defect site was stained with IHC. Due to the difference in the degree of spinal cord repair in each group, there were differences in the area and morphology of immunohistochemistry sections in each group. Scale bars = 50 μm. b The IOD value of the IHC images in each group; *p < 0.05, **p < 0.01. All groups were compared with Sham group. c Western blotting bands of Tuj1, GFAP, ChAT, Islet-1, and HB9 of the NE-4C cells in Sham, SCI, GO/PVA, MoS 2 /PVA, and MoS 2 /GO/PVA groups at 6 weeks postoperatively. d Gray values of western blotting in each group; *p < 0.05. All groups were compared with the Sham group

Article Snippet: The samples were then incubated overnight with primary antibodies: rabbit anti-HB9 antibody (ORB157435, 1:1000, Biorbyt), rabbit anti-Islet-1 antibody (GTX 102807, 1:1000, Genetex), rabbit anti-ChAT antibody (PA5, 1:10000, Thermo Fisher), rabbit anti-GFAP antibody (GTX108711, 1:1000, Genetex), and rabbit anti-Tuj1 (GTX130245, 1:1000, Genetex) at 4 °C.

Techniques: Staining, Immunohistochemistry, Western Blot

Journal: PLoS Genetics

Article Title: A Mutation in the Mouse Ttc26 Gene Leads to Impaired Hedgehog Signaling

doi: 10.1371/journal.pgen.1004689

Figure Lengend Snippet: ( A ) Representative images of hop/+ and hop/hop mice at postnatal day 4. ( B ) Comparison of Alcian Blue-stained fore and hind limbs of hop/+ and hop/hop mice. Extra digits are indicated by arrows. ( C ) Statistical analysis of FoxA2-positive cells in the lumbar neural tubes of hop/+ and hop/hop mice (E10.5). Each symbol represents the average number of FoxA2 + cells per focal plane in a single embryo (for each embryo, 12 focal planes in 4 sections were analyzed, Mann-Whitney test: * P = 0.01). ( D ) Immunostaining of the lumbar neural tube of hop/+ and hop/hop mice (E10.5) with antibodies against the V3 progenitor marker Nkx2.2 (white contrast), the floor plate marker FoxA2 (red), and the motor neuron protein HB9 (white contrast). The hop/hop genotype is associated with reduced FoxA2 expression and ventralization of the Nkx2.2- and HB9-expressing cells. Scale bar: 50 µm. ( E ) Representative ABR waveforms for 3–4 week-old hop/+ and hop/hop mice. Broadband click stimuli were applied at the indicated sound pressure levels (in dB). ( F ) Statistical analysis of ABR thresholds measured in 3–4 week-old hop/+ and hop/hop mice. Broadband click stimuli between 30 and 90 dB sound pressure level (SPL) were used. Each symbol represents the value for a single mouse (Mann-Whitney test: *** P <0.0001).

Article Snippet: Transverse cryosections through the lumbar region were re-fixed with pre-chilled 4% PFA for 10 min at RT, permeabilized with 0.1% Triton X-100 for 10 min, blocked with 5% normal goat serum (Sigma) and incubated with antibodies against FoxA2 (Abcam; 1∶250 dilution), HB9 (Developmental Studies Hybridoma Bank, University of IA; 1∶30 dilution), Nkx2.2 (Developmental Studies Hybridoma Bank, University of IA; 1∶10), acetylated α-tubulin (1∶1000, Sigma), or Arl13b (1∶500, Protein Tech).

Techniques: Comparison, Staining, MANN-WHITNEY, Immunostaining, Marker, Expressing