Journal: The American Journal of Pathology

Article Title: MDM2

doi: 10.2353/ajpath.2006.051351

Figure Lengend Snippet: A: Representative RT-PCR analysis of total RNA from cultured human VSMCs (HASMC). RNA was amplified in the presence of oligonucleotide primers specific for MR (top panel) and 11β-HSD type 2 (bottom panel). Extracts from human kidney were used as a positive control (P). B: Representative immunoblotting studies demonstrating MR and 11β-HSD type 2 proteins in HASMC. Extracts from human kidney were used as a positive control (P). C and D: Immunocytochemical analysis demonstrated that immunopositive cells for MR appear brown as a result of diaminobenzidine colorimetric reaction in HASMC without ligand stimulation (C) or treated with 10 nmol/L aldosterone (D) (Original magnification, ×400).

Article Snippet: Cell Culture and Characterization A cultured human VSMC cell line, ie, HASMC (derived from human abdominal aorta) was commercially obtained from Kurabo Corp. (Osaka, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Cell Culture, Amplification, Positive Control, Western Blot

Journal: BMC Complementary and Alternative Medicine

Article Title: Protective activity ethanol extract of the fruits of Illicium verum against atherogenesis in apolipoprotein E knockout mice

doi: 10.1186/s12906-015-0750-0

Figure Lengend Snippet: Illicium v. inhibits inflammatory gene expression in TNF-α-stimulated human aortic smooth muscle cells. a Human aortic smooth muscle cells (HASMC) were treated with or without Illicium v. (10–100 μg/mL) for 24 h. After incubation, cell viability was determined using the MTT assay. b HASMCs were transiently transfected with pGL4.32-NF-κB, a renilla luciferase control reporter vector, and then treated with or without Illicium v. (10–100 μg/mL), followed by incubation with 10 ng/mL TNF-α for 24 h. NF-κB transcription activity was determined using a luciferase reporter assay. c Protein samples from HASMCs, treated with or without Illicium v. (10–100 μg/mL) and 10 ng/mL TNF-α for 24 h, examined by western blot analysis for NF-κB protein expression. d Protein expression of adhesion molecules (ICAM, VCAM, and E-selectin). All data represent mean values ± SEM. ** P < 0.01, *** P < 0.001 versus control; # P < 0.05, ## P < 0.01, ### P < 0.001 versus HFD

Article Snippet: Cryopreserved HASMC were purchased from ScienCell Research Laboratory (San Diego, CA, USA).

Techniques: Gene Expression, Incubation, MTT Assay, Transfection, Luciferase, Control, Plasmid Preparation, Activity Assay, Reporter Assay, Western Blot, Expressing