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Developmental Studies Hybridoma Bank
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Chem Impex International
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R&D Systems
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Elabscience Biotechnology
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OriGene
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Cyagen Biosciences
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R&D Systems
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Thermo Fisher
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Aviva Systems
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Genecopoeia
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Thermo Fisher
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Image Search Results
Journal: Journal of visualized experiments : JoVE
Article Title: Design and Microinjection of Morpholino Antisense Oligonucleotides and mRNA into Zebrafish Embryos to Elucidate Specific Gene Function in Heart Development
doi: 10.3791/63324
Figure Lengend Snippet: Embryonic survival for different Hand2 MO concentrations was converted to a percentage, averaged, and plotted. The percent survival of MO-injected embryos was calculated at 24, 48, and 72 hpf. All data points represent mean ± SEM (100 embryos were used in each group; experiment was performed in triplicate). The analysis was done by two-way-ANOVA with Dunnett test. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
Article Snippet:
Techniques: Injection
Journal: Journal of visualized experiments : JoVE
Article Title: Design and Microinjection of Morpholino Antisense Oligonucleotides and mRNA into Zebrafish Embryos to Elucidate Specific Gene Function in Heart Development
doi: 10.3791/63324
Figure Lengend Snippet: Hearts and tails of 72 hpf embryos were examined under a light microscope (100x magnification). (A,B) Control scrambled MO shows normal heart development without pericardial edema. (C,D) 0.4 mM HAND2 MO-injected embryos as positive control shows tube-like heart structure.
Article Snippet:
Techniques: Light Microscopy, Control, Injection, Positive Control
Journal: Journal of visualized experiments : JoVE
Article Title: Design and Microinjection of Morpholino Antisense Oligonucleotides and mRNA into Zebrafish Embryos to Elucidate Specific Gene Function in Heart Development
doi: 10.3791/63324
Figure Lengend Snippet: (A) Dorsal aorta blood flow analysis, (B) Posterior Cardinal Vein (PCV) blood flow analysis. 1–4 cell stage zebrafish embryos were injected as groups with scrambled MO, Hand2 MO, Hand2 MO + Hand2 mRNA rescue, and scrambled MO + Hand2 mRNA rescue. Un-injected embryos were used as control. All data are presented as mean ± SEM (6 embryos were used in each group; experiment was performed in triplicate). The analysis was done by one-way ANOVA with Sidak post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.
Article Snippet:
Techniques: Injection, Control
Journal: Journal of visualized experiments : JoVE
Article Title: Design and Microinjection of Morpholino Antisense Oligonucleotides and mRNA into Zebrafish Embryos to Elucidate Specific Gene Function in Heart Development
doi: 10.3791/63324
Figure Lengend Snippet: Materials
Article Snippet:
Techniques: Imaging, Electrochemiluminescence, Plasmid Preparation, Extraction, Spectrophotometry, Software, Microscopy, Acid Assay, Bicinchoninic Acid Protein Assay, Protease Inhibitor, Radio Immunoprecipitation, Control, Mutagenesis, Stripping Membranes